Quasar 670 CE Phosphoramidite | CAS 1032678-33-3

Quasar 670 CE Phosphoramidite is a fluorescent, click-compatible phosphoramidite building block designed for incorporation into oligonucleotides and other nucleic-acid-derived constructs. As a reagent platform for Cu-free click chemistry workflows, it enables post-synthetic functionalization or conjugation strategies that are widely used in chemical biology and imaging tool development. The Quasar 670 fluorophore provides strong far-red emission suitable for multiplexing and low-background detection in labeling experiments, while the phosphoramidite format supports straightforward integration into DNA or RNA sequences for downstream conjugation.

1. DNA Probe Labeling

Quasar 670 CE Phosphoramidite is commonly used to generate fluorescent DNA probes for hybridization-based assays and nucleic-acid tracking experiments in research laboratories. By building the fluorophore into the oligonucleotide scaffold via the phosphoramidite handle, investigators can create sequence-defined probes that maintain consistent labeling stoichiometry across batches. The click-reactive functionality further supports modular workflows where the labeled probe is later coupled to capture surfaces, affinity handles, or secondary reporter components, enabling robust assay development for genomics workflows, nucleic-acid imaging, and probe optimization studies.

2. Bioorthogonal Conjugation Platforms

Quasar 670 CE Phosphoramidite fits into bioorthogonal conjugation strategies where fluorescent nucleic-acid constructs are linked to biomolecular targets, polymers, or engineered surfaces using click chemistry-compatible handles. Researchers in chemical biology and biomaterials science use this reagent to prepare clickable oligonucleotide reagents that can be assembled into larger functional systems without relying on harsh post-labeling conditions that may compromise biomolecule integrity. The far-red Quasar 670 signal is particularly useful when constructing multi-component labeling schemes, such as fluorescent tagging of macromolecular assemblies, affinity reagents, and imaging-ready conjugates.

3. Molecular Imaging Reagents

Quasar 670 CE Phosphoramidite is used to prepare imaging reagents that rely on sequence-defined fluorescent probes and subsequent click-based attachment to imaging scaffolds. In molecular imaging and advanced microscopy workflows, investigators often require stable, bright labeling with emission in a spectral window that reduces interference from cellular autofluorescence; Quasar 670 supports this need while remaining compatible with oligonucleotide synthesis pipelines. The phosphoramidite format enables reproducible incorporation into probes or targeting oligos, and the click-compatible functionality supports conjugation to imaging platforms such as fluorescent reporters on materials, imaging probes with multivalent architectures, or assay formats that benefit from modular assembly.

4. Diagnostic Reagent Development

Quasar 670 CE Phosphoramidite is frequently selected for development of research diagnostic reagents that use fluorescent oligonucleotides as detection elements. Teams building nucleic-acid detection assays, biosensing formats, and laboratory diagnostic prototypes leverage the phosphoramidite chemistry to produce consistent, sequence-specific fluorescent reagents that can be integrated into assay workflows. Click chemistry compatibility supports downstream coupling to assay surfaces, immobilization chemistries, and reporter architectures, allowing developers to tailor assay design while maintaining a defined fluorescent readout for sensitive signal generation in benchtop testing environments.