Fluorescence Immunoassay (FIA)
Fluorescent immunoassay (FIA), known for its high sensitivity and specificity, has found wide applications in key research areas such as disease detection, protein localization, cell imaging, and high-throughput screening. The choice of fluorescent dyes and probes, along with their labeling efficiency, directly determines the reliability and resolution of fluorescent immunoassays. BOC Sciences, with its robust molecular design capabilities, synthesis platforms, and quality control systems, offers a range of high-performance fluorescent dyes, probe kits, and customized services to global research institutions and corporate clients. These offerings support every step of fluorescent immunoassay research and application. Our product line includes organic small molecule dyes, Cyanine dyes, Alexa Fluor dyes, and multicolor labeling systems, which are widely applicable in flow cytometry, immunofluorescence (IF), immunohistochemistry (IHC), and fluorescence in situ hybridization (FISH).
What is a Fluorescent Immunoassay?
Fluorescent immunoassay (FIA) is a detection technology based on the specific antigen-antibody binding reaction, where antibodies or antigens are labeled with fluorescent dyes to enable the quantitative or qualitative detection of target molecules. The principle involves introducing a fluorescent label into the immunoreaction system. When the fluorescent-labeled antibody or antigen binds to the target molecule, the fluorophore emits a fluorescence signal upon excitation with specific wavelengths of light, and the signal intensity is read and analyzed using fluorescence detection equipment. The fluorescence intensity is typically directly correlated with the target molecule's concentration, allowing for high-sensitivity detection. Compared to traditional enzyme-linked immunosorbent assay (ELISA), fluorescent immunoassay offers higher sensitivity and a broader detection dynamic range, making it particularly suitable for detecting low-abundance biomarkers. Additionally, FIA is compatible with platforms like flow cytometry, multicolor imaging, and microarray chips, providing high throughput and automation potential. Due to its strong specificity, ease of use, and intuitive results, fluorescent immunoassay is widely used in disease diagnosis, biomarker screening, drug development, environmental monitoring, and food safety, becoming one of the most important tools in modern bioanalysis and life sciences research.
Advantages of BOC Sciences in Fluorescent Immunoassays Solutions
Comprehensive Dye Platform
Our platform covers a wide range of fluorescent dyes, from ultraviolet to near-infrared wavelengths, adaptable to various detection systems and experimental conditions.Strong Independent Synthesis Capabilities
With mature synthesis routes and structural modification platforms, we offer custom dye structures, active group introduction, and spectral modulation.Professional Labeling Service Team
We provide efficient, stable, and high-sensitivity antibody labeling services, supporting multicolor immunoassays.Rigorous Quality Control System
All dye products undergo multiple quality control checks, including purity, photostability, and fluorescence intensity, ensuring experimental reproducibility and data reliability.
Fluorescent Dye Solutions to Meet Diverse Immunoassays Needs
To meet the diverse needs of researchers in fluorescent immunoassays, BOC Sciences provides a range of high-performance dye solutions. These dyes are available in various wavelength ranges, with strong photostability and high labeling efficiency, suitable for multicolor analysis, precise quantification, and molecular detection in complex systems. Our fluorescent dye products are widely used in antibody labeling, protein analysis, cell imaging, immunohistochemistry, and flow cytometry, particularly suited for various fluorescent immunoassay experimental setups. Core product lines include:
Organic Fluorescent Dyes
- FITC
- Rhodamine B
- Texas Red-X
- NBD-Cl (4-Chloro-7-nitrobenzofurazan)
- Dansyl chloride
Alexa Fluor Series Dyes
- Alexa Fluor 488
- Alexa Fluor 546
- Alexa Fluor 594
- Alexa Fluor 647
- Alexa Fluor 750
Cyanine Series Dyes
Comprehensive Customization Services for Precise Immunoassay Detection
With the increasing trend toward high-throughput, multiplex, and personalized fluorescent immunoassays, standardized products often fail to fully meet the high demands for sensitivity, specificity, and compatibility. Leveraging our extensive fluorescent dye R&D experience and strong technical support team, BOC Sciences offers a comprehensive set of customization services. We are dedicated to providing more targeted solutions for research institutions, biotech companies, and drug development firms. From fluorescent antibody labeling to multicolor detection optimization, exclusive probe design to high-throughput screening reagent development, we help clients optimize signals and reduce background noise, effectively improving detection accuracy and experimental efficiency, thereby advancing precise immunoassay detection and disease research.
Antibody Fluorescent Labeling Services
We provide one-on-one antibody fluorescent labeling solutions, covering optimization of dye-antibody ratios, label stability verification, and application compatibility assessment. Services support IgG, Fab, nanobodies, and other antibody types.
Fluorescent Probe Development Services
Utilizing our robust molecular design platform, we design and synthesize fluorescent probes targeting specific enzymes, receptors, and proteins, suitable for signal pathway studies and live-cell dynamic imaging.
Multicolor Detection Optimization Consulting
For multicolor flow cytometry or confocal microscopy, BOC Sciences offers recommendations on fluorescent dye combinations and compensation settings, helping clients optimize multi-parameter detection systems and avoid spectral overlap.
High-Throughput Screening Reagent Development
For drug screening and functional genomics research, we provide high-throughput-compatible fluorescent probes and labeled antibody solutions, accelerating the screening process and improving detection sensitivity and reproducibility.
One-Stop Fluorescence Immunoassay Kit Development Services
In addition, BOC Sciences offers comprehensive fluorescent probe kit development services, focusing on providing customers with efficient and sensitive detection tools. We design the optimal combination of fluorescent probes based on target molecules, sample types, and experimental conditions, supporting both single-labeling and multiplex labeling applications to ensure high specificity and sensitivity. Our services include probe screening, optimization, bulk reagent kit production, and quality control, ensuring the stability and consistency of each product. All developed reagent kits undergo rigorous performance verification and meet the high standards required for both research and industrial applications. We also offer customized packaging and user manuals, ensuring ease of use and reliable results. By collaborating with BOC Sciences, you will receive professional fluorescent probe reagent kits that provide strong support for immunoassays and bioanalyses.
Strict Quality Control System Ensures Dye Quality in Every Batch
In the development and production of fluorescent dyes, quality control is a critical step to ensure that every batch meets high standards. BOC Sciences conducts rigorous quality testing on each batch of fluorescent dyes to ensure that the product's stability, sensitivity, purity, and optical properties are at their best. We employ an advanced quality management system and adhere to multiple international certification standards, covering the entire process from raw material procurement to final product delivery. Whether used for high-throughput screening, immunoassays, or cell imaging, we ensure that every batch of dye provides consistent and reliable experimental data for our customers.
- Light Absorption and Emission Spectrum Analysis: Fluorescent dyes' absorption and emission wavelength ranges are measured using a UV-Vis spectrophotometer to ensure the dye absorbs sufficiently at the desired wavelength and exhibits good emission intensity to meet different experimental needs.
- Purity Analysis: High-performance liquid chromatography (HPLC) and gas chromatography (GC) are used to detect the purity of the dye, ensuring no impurities or unstable components are present, thus guaranteeing the accuracy of experimental results.
- Solubility and Stability Testing: Dyes are tested for solubility in different solvents and their stability during long-term storage to ensure uniformity and reliability during use, preventing negative effects due to poor solubility.
- Molecular Weight and Structural Identification: Mass spectrometry (MS) and nuclear magnetic resonance (NMR) are used to identify the molecular weight and structure of the dye to ensure it meets design standards and avoids experimental biases caused by structural abnormalities.
- pH and Ionic Strength Adaptability: Testing is conducted on the stability of dyes under different pH values and ionic strengths to ensure they perform well under various experimental conditions, preventing performance fluctuations due to environmental factors.
- Color/Fluorescence Intensity Measurement: Fluorescence intensity is measured using a fluorescence spectrophotometer to ensure the dye has enough luminescent intensity to improve the signal-to-noise ratio and provide clear detection signals at low concentrations.
- Fluorescence Decay Time: The decay time of fluorescent dyes is measured to ensure an appropriate lifespan, providing stable fluorescence signals during experiments and reducing errors caused by signal decay.
Advanced Detection Platforms
- High-Performance Liquid Chromatography (HPLC)
- Liquid Chromatography-Mass Spectrometry (LC-MS)
- UV-Vis Spectrophotometer
- Fluorescence Spectrophotometer
- Mass Spectrometer (MS)
- Dynamic Light Scattering (DLS)
- Nuclear Magnetic Resonance (NMR)
- High-Resolution Microscopes
Applied to Multiple Fluorescent Immunoassay Research Types
BOC Sciences' fluorescent dye and probe solutions are widely applied in various fluorescent immunoassay research fields, covering multiple aspects from basic research to clinical applications. Whether for immunohistochemistry, immunohistochemical analysis, or multiplex detection platforms, our products provide precise, sensitive, and reliable support. Our fluorescent labeling technology and customization services help researchers obtain high-quality data at the cellular and molecular levels, advancing disease diagnosis, drug development, and biomarker discovery.
Immunohistochemistry (IHC)
Immunohistochemistry uses the specific binding of antibodies and antigens, combined with fluorescent dyes, to label and analyze tissue sections. BOC Sciences' fluorescent dyes efficiently label target molecules and provide precise cellular and tissue structure information through fluorescence microscopy, helping researchers observe and identify antigen distribution in tissue samples.
Immunofluorescence Microscopy
Immunofluorescence microscopy is used to observe the expression of specific proteins within or between cells by binding fluorescently labeled antibodies to target molecules. Our fluorescent dyes offer high brightness and low background, making cellular structure and function research more accurate, widely applied in cell biology, molecular biology, and other fields.
Flow Cytometry (FACS)
Flow cytometry is a high-throughput cell analysis technology used to detect and analyze multiple cell features. BOC Sciences' fluorescent dyes are widely used in multiplex flow cytometry analysis by labeling different cells or molecules with fluorescent dyes to enable rapid and accurate analysis of cell phenotypes, immune responses, and other biomarkers.
Protein Interaction Research
Fluorescent immunoassays are also widely used in studying protein-protein interactions, using techniques like fluorescence resonance energy transfer (FRET) to detect physical contacts and interactions between two or more proteins in cells. Our fluorescent dyes provide high sensitivity and accuracy, enabling deeper exploration of protein-protein interactions.
Fluorescence In Situ Hybridization (FISH)
FISH technology is widely used in genomic analysis, chromosomal abnormality detection, and gene expression research, being an essential tool for accurate diagnosis and genetic research. BOC Sciences provides fluorescent probes targeting specific nucleic acid sequences, helping researchers accurately localize gene expression, mutations, and chromosomal structural changes.
FAQs About Fluorescence Immunoassay
What is the fluorescence method of immunoassay?
The fluorescence method of immunoassay is a sensitive analytical technique that utilizes fluorescent labels to detect specific biomolecules, typically antibodies or antigens, in a sample. In this method, a fluorescent compound is conjugated to an antibody or antigen, and the sample is exposed to light at a specific wavelength. The compound absorbs the energy and emits light at a longer wavelength, which is detected by specialized instruments. This technique is highly sensitive, allowing for the detection of low-abundance targets, and is widely used in diagnostics, research, and therapeutic monitoring.
What is fluorescence linked immunosorbent assay FLISA?
Fluorescence linked immunosorbent assay (FLISA) is a type of immunoassay that uses a fluorescently labeled antibody to detect the presence and concentration of a specific antigen in a sample. It works similarly to an enzyme-linked immunosorbent assay (ELISA), but instead of an enzyme, a fluorescent molecule is used as the detection label. When the antigen binds to the antibody on a solid phase, the fluorescent signal generated upon excitation is measured, offering high sensitivity and quantification capabilities. FLISA is commonly used in clinical diagnostics and research applications for its precision and low detection limits.
What is the principle of FIA?
The principle of fluorescence immunoassay (FIA) is based on the specific binding of an antigen to its corresponding antibody, where one of the components is labeled with a fluorescent dye. When exposed to light of a specific wavelength, the fluorescent label absorbs the energy and emits light at a longer wavelength. This emitted fluorescence is then measured to determine the presence and quantity of the antigen or antibody. FIA offers high sensitivity due to the strong and measurable fluorescent signal, making it ideal for detecting low concentrations of analytes in complex biological samples.
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