Cy7-NHS ester | 477908-53-5

Cy7-NHS ester, a versatile fluorescent dye, finds applications in labeling proteins, peptides, and other biomolecules for a multitude of uses. Below are four key applications of Cy7-NHS ester:

Fluorescence Imaging: Widely utilized in fluorescence imaging, Cy7-NHS ester emits near-infrared light that penetrates deep into tissues with minimal background interference. Scientists leverage this dye to visualize intricate cellular structures, track labeled biomolecules, and monitor dynamic biological processes in real time. Particularly vital in in vivo imaging, where clear and detailed images are paramount for unraveling disease progression and assessing treatment efficacy.

Flow Cytometry: A favored choice for conjugation in flow cytometry, Cy7-NHS ester’s distinct emission spectrum enables multiparametric analysis, facilitating the simultaneous examination of numerous cell surface markers. This application is indispensable in immunology and related fields where precise identification and characterization of cell populations are essential for advancing research and understanding complex immune responses.

Western Blotting: In protein studies, Cy7-NHS ester plays a crucial role in enhancing the sensitivity of protein detection in Western blotting experiments. Its near-infrared fluorescence minimizes background noise, improving the signal-to-noise ratio for accurate quantification and visualization of protein bands. This capability is particularly valuable when analyzing proteins present in low abundance, enhancing the accuracy and reliability of experimental results.

In Vivo Tracking: Employed for tracking the biodistribution of biomolecules, cells, or nanoparticles within living organisms, Cy7-NHS ester enables researchers to monitor the movement and localization of these entities over time using specialized imaging techniques. This application is pivotal in preclinical research, providing insights into pharmacokinetics, cell migration, and therapeutic targeting, ultimately advancing our understanding of complex biological processes and aiding in the development of targeted therapies.