RNA Staining
As a top biochemistry supplier, BOC Sciences presents numerous fluorescent dyes that are specialized for RNA staining applications. RNA staining technology represents a fundamental technique in molecular biology research that researchers commonly use for RNA extraction, quantification, purification, and structural analysis. Our cutting-edge fluorescent dyes enable researchers to visualize RNA molecules effectively while producing accurate and efficient experimental results. Our fluorescent dyes provide tailored solutions for specific client requirements while maintaining adaptability for multiple experimental purposes in complex research contexts.
What is RNA Staining?
RNA staining involves applying specific dyes or markers to RNA molecules to visualize them through fluorescence microscopy or other imaging technologies. RNA staining presents more difficulties than DNA staining because RNA molecules demonstrate less stability and have a greater tendency to undergo degradation. Currently, RNA staining serves multiple purposes, such as analyzing gene expression patterns, studying RNA localization, performing RNA sequencing, and carrying out polymerase chain reaction (PCR) methods. Thus, RNA staining gives researchers superior sensitivity and resolution for RNA studies and enables detailed examination of biological processes at the RNA level in cells.
Fig. 1. Fluorescence in situ hybridization for RNA visualization (BOC Sciences Authorized).
Proven Track Record in Fluorescent Reagent Success
High Specificity and Affinity
The fluorescent dyes provided by BOC Sciences exhibit excellent RNA binding capabilities, enabling efficient staining at low concentrations while minimizing non-specific background signals, thus improving detection sensitivity.Outstanding Optical Performance
Fluorescent dyes for RNA have different spectral properties, and BOC Sciences offers dyes across UV, visible light, and near-infrared regions to meet various experimental needs. These dyes also have high photostability and high quantum yield, maintaining strong fluorescence signal over time.Compatibility with Multiple Application Platforms
The fluorescent dyes from BOC Sciences are suitable for various technical platforms, including fluorescence microscopy, flow cytometry, real-time fluorescence detection, nucleic acid hybridization, and biosensors. They can also be customized to suit specific RNA research needs.Biocompatibility and Low Cytotoxicity
To support live cell imaging and in vivo RNA tracking, BOC Sciences has optimized the chemical structure of the dyes to improve biocompatibility and reduce cytotoxicity, ensuring the reliability of experiments.
Explore Our Popular RNA Stain Lists
BOC Sciences offers advanced skills for creating tailor-made fluorescent dyes that enable us to design and produce specific dyes according to their clients' special requirements. Our fluorescent dye selection features SYBR Green, Ethidium Bromide, and Acridine Orange, which exhibit high sensitivity and photostability with minimal background noise. In addition, we have launched new fluorescent dyes like near-infrared fluorescent dyes and multi-color fluorescent dyes to serve the various research requirements across different fields. The extensive experience of our R&D team combined with our advanced technology allows us to deliver tailored, high-quality solutions promptly according to customer requirements.
| Dye Name | Excitation/Emission Wavelength | Applications |
| SYTO 9 | 485 nm / 498 nm | Used for RNA labeling, suitable for real-time monitoring and quantitative analysis. |
| Syto RNASelect | 500 nm / 530 nm | High-sensitivity RNA labeling, suitable for fluorescence microscopy and flow cytometry. |
| Acridine Orange (AO) | 492 nm / 525 nm | Used for RNA and DNA labeling, suitable for real-time detection and cell staining. |
| Thiazole Orange (TO) | 520 nm / 550 nm | Suitable for real-time RNA monitoring and nucleic acid detection. |
| QuantiFluor™ RNA Dye | 494 nm / 520 nm | Used for RNA quantification, suitable for real-time PCR and other quantitative assays. |
| RNA-Scope® Probe | 550 nm / 570 nm | Used for in situ hybridization to detect specific RNA molecules. |
| Ethidium Bromide | 302 nm (UV) / 590 nm | Used for DNA and RNA gel electrophoresis, common in gel staining and visualization under UV light. |
| FAM | 494 nm / 520 nm | Used for RNA probe labeling, suitable for real-time PCR and fluorescence microscopy. |
| Alexa Fluor 488 | 495 nm / 519 nm | Used for RNA labeling, suitable for flow cytometry and fluorescence microscopy analysis. |
| Alexa Fluor 594 | 590 nm / 617 nm | Used for RNA labeling, commonly used in multiplex labeling experiments and fluorescence imaging. |
| SYBR Gold | 495 nm / 535 nm | High-sensitivity RNA dye, suitable for RNA electrophoresis and real-time PCR analysis. |
| SYBR Green I | 497 nm / 520 nm | Widely used for RNA and DNA detection, commonly used in real-time PCR analysis. |
| SYBR Green II | 500 nm / 520 nm | Used for RNA quantification, commonly used in real-time PCR. |
| SYBR Safe | 302 nm (UV) / 530 nm | Used for RNA and DNA gel electrophoresis, with lower toxicity and strong fluorescence signal. |
Personalized RNA Application Development and Technical Support
BOC Sciences offers comprehensive customized RNA fluorescent probe development services aimed at meeting the personalized needs of clients in research and experiments. Our services are not limited to standardized RNA fluorescent dyes, but also include customized development for various specific requirements, ensuring high precision and broad application of the probes. Below are the details of our customized services:
RNA-Specific Probe Design and Synthesis
We provide tailored RNA-specific probe design and synthesis services based on the client's research needs. Through precise design, we ensure the probe can specifically recognize and bind to the target RNA molecules, minimizing non-specific binding, and enhancing the sensitivity and accuracy of experiments.
Fluorescent Labeling Modifications
In the development of RNA fluorescent probes, labeling modifications are crucial for enhancing the probe's performance. We offer various fluorescent labeling modifications, including PEGylation (polyethylene glycolylation) and amidation, to improve the probe's stability, solubility, and cell compatibility.
Multi-Platform Compatibility
Whether it is flow cytometry, real-time quantitative PCR (qPCR), fluorescence microscopy, or other platforms, we can provide customized dyes to ensure the optimal performance of the fluorescent signal on the target detection platform.
Fluorescent Dyes Supporting RNA Gel Electrophoresis
RNA gel electrophoresis is a commonly used RNA separation and detection technique, where fluorescent dyes play a vital role. The fluorescent dyes provided by BOC Sciences can specifically bind to RNA molecules and emit strong fluorescence under UV or blue light excitation, facilitating the observation and analysis of RNA bands. Our dyes feature high sensitivity and low toxicity, making them suitable for various types of RNA gel electrophoresis, including agarose gel electrophoresis and polyacrylamide gel electrophoresis. By using our fluorescent dyes, researchers can accurately detect the molecular weight, purity, and integrity of RNA, providing reliable data support for subsequent research. Explore Our Support Services
Rigorous Quality Control for Optimal Fluorescent Stain Performance
At BOC Sciences, we prioritize quality control in the production and application of fluorescent dyes. To ensure that every batch of fluorescent dye meets the highest quality standards, we implement a series of strict quality control measures and use advanced instrumentation. These measures not only guarantee the dye’s performance and stability but also ensure its reliability in RNA staining and related experiments.
- Strict Raw Material Screening: All incoming raw materials undergo rigorous inspection to ensure compliance with international quality standards.
- Fluorescent Dye Batch Testing: Each batch of fluorescent dye is subjected to stringent batch testing, including assessments of fluorescence intensity, wavelength, solubility, and stability.
- Fluorescence Performance Evaluation: A fluorescence spectrometer is used to comprehensively test the excitation and emission wavelengths of the dye, ensuring strong and stable fluorescence signals under different excitation light sources.
- Optimal Storage Conditions: During storage and transportation, dyes are kept in low-temperature and dry conditions to maintain long-term stability.
- Stability and Shelf-Life Testing: Long-term stability tests are conducted to periodically monitor fluorescence intensity decay, ensuring consistent performance over extended storage and use.
- Batch Traceability and Full Process Monitoring: Each batch of dye is accompanied by a detailed quality report, documenting every stage of production and test data.
- Final Product Inspection: Before shipment, the final product undergoes a series of inspections, including solubility, concentration, fluorescence intensity, and batch consistency testing.
Advanced Analytical Platform
- UV-Vis Spectrophotometer
- GC
- AAS
- Melting Point Apparatus
- FTIR
- GPC
- ICP-MS
- Polarimeter
- NMR
- TLC
- XRF
- Viscometer
- Fluorescence Spectroscopy
- LC-MS
- DSC
- XRD
- HPLC
- GC-MS
- TGA
- Karl Fischer Titration
Comprehensive Technical Support for Fluorescent Dye Applications
Our fluorescent dyes offer extensive support in RNA staining research applications. In addition to common RNA quantification and localization studies, our dyes are widely used in areas such as RNA splicing, transcriptional regulation, and RNA-protein interaction research. Our products meet various experimental needs, including real-time PCR, in situ hybridization, flow cytometry, and immunofluorescent labeling. Moreover, our fluorescent dyes support high-throughput screening technologies, playing a crucial role in large-scale RNA screening and gene expression analysis. With BOC Sciences’ fluorescent dyes, researchers can conduct RNA-related studies more efficiently and accurately, advancing progress in the life sciences field.
RNA Gel Electrophoresis Analysis
RNA gel electrophoresis is an important tool for studying RNA molecule size, purity, and integrity. The fluorescent dyes from BOC Sciences can effectively label RNA, providing clear fluorescence signals that help researchers accurately separate RNA molecules of different sizes during gel electrophoresis. Compared to traditional dyes, fluorescent dyes offer higher sensitivity and lower background noise, making RNA visualization more precise.
Real-Time Quantitative PCR (qPCR) Analysis
Real-time quantitative PCR (qPCR) is widely used in RNA quantification, especially in gene expression research. BOC Sciences’ fluorescent dyes offer high sensitivity and specificity, enabling precise detection and quantification of specific RNA molecules. With fluorescent dyes compatible with qPCR equipment, efficient and accurate gene expression analysis can be achieved, supporting extensive transcriptomic research.
RNA Capture and Quantification Analysis
RNA capture and quantification are key experimental steps, especially in mRNA research. The fluorescent dyes provided by BOC Sciences can efficiently label RNA molecules and specifically bind to capture probes, enabling RNA isolation and quantification. This process is widely applied in gene expression analysis, genomics, and cancer research.
RNA Modification Studies
Various chemical modifications on RNA molecules (such as methylation and pseudouridylation) significantly impact their function and stability. The fluorescent dyes from BOC Sciences can specifically bind to these modifications, providing reliable tools for RNA modification research. These dyes support efficient labeling of RNA modifications in various cell and animal models, advancing studies in epigenetics and post-transcriptional regulation.
RNA Virus Research
Fluorescent dyes have significant applications in RNA virus research, especially in the localization and analysis of viral genomes. The fluorescent dyes provided by BOC Sciences can effectively label RNA virus genomes, helping researchers track viral replication processes and the distribution of viral RNA in host cells. This is crucial in vaccine development, virus mechanism research, and antiviral drug screening.
FAQs About RNA Staining
What stain is used for RNA?
Common RNA stains include SYBR Green, SYBR Gold, EtBr (Ethidium Bromide), and Methylene Blue. These dyes bind to RNA molecules and emit fluorescence under UV light, making detection easier.
What color does RNA stain?
RNA, when stained with fluorescent dyes like SYBR Green or SYBR Gold, typically emits green fluorescence. SYBR Green emits green fluorescence under UV light, while SYBR Gold emits stronger golden or green fluorescence. Ethidium Bromide (EtBr) is also commonly used and stains RNA to emit red or orange fluorescence. These fluorescent dyes significantly enhance fluorescence intensity, making them widely used in gel electrophoresis or quantitative PCR.
Does Coomassie Blue stain RNA?
Coomassie Blue is a dye commonly used for protein staining. It interacts with amino acid residues in proteins to produce a blue reaction. However, Coomassie Blue is not suitable for RNA staining because the molecular characteristics of RNA differ from those of proteins, and Coomassie Blue cannot form stable complexes with RNA molecules.
Does SYBR Gold stain RNA?
Yes, SYBR Gold is a highly sensitive fluorescent dye that efficiently stains RNA. When SYBR Gold binds to RNA, it emits intense green or golden fluorescence under UV light, making it widely used in RNA detection, particularly in RNA gel electrophoresis and quantitative PCR.
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