Technical Articles

Technical Articles

Lipid Staining: Definition, Principles, Methods, Dyes, and Uses

Lipid Staining: Definition, Principles, Methods, Dyes, and Uses

2024-12-21

Lipid staining is a key technique in biological research used to identify and analyze the distribution of lipids within cells or tissues. Lipids play an important role in cell membrane structure, energy storage, and signal transduction. However, their unique hydrophobicity makes them difficult to visualize using conventional staining methods. Lipid staining utilizes lipophilic dyes that specifically label lipids, helping researchers observe lipid morphology, distribution, and metabolic changes. This technique is widely used in the study of metabolic diseases such as obesity, fatty liver, and atherosclerosis, as well as in cancer research, neurobiology, and plant biology.


Flow Cytometry: Definition, Principles, Protocols, Dyes, and Uses

Flow Cytometry: Definition, Principles, Protocols, Dyes, and Uses

2024-12-15

Flow cytometry (FCM) is a technique for rapidly analyzing cell characteristics in a liquid flow. It enables multi-parameter quantitative analysis of single cells by combining them with specific antibodies, measuring attributes such as cell surface markers, intracellular protein expression, cell cycle, and DNA content. Flow cytometry offers advantages such as high throughput, high sensitivity, and high specificity, making it widely applicable in fields like immunology, hematology, and oncology.


Nucleic Acid Staining: Definition, Principles, Dyes, Procedures, and Uses

Nucleic Acid Staining: Definition, Principles, Dyes, Procedures, and Uses

2024-12-03

Nucleic acid dyes are compounds that can bind to nucleic acids and are commonly used for the quantification and detection of nucleic acids. These dyes are generally aromatic compounds containing benzene rings or pyridine rings. These ring structures absorb ultraviolet or blue light and emit fluorescence. Nucleic acid molecules are composed of nucleotide units, which consist of a five-carbon sugar, a nitrogenous base, and a phosphate group. DNA and RNA differ, but both contain four types of bases. Nucleic acid dyes typically stain by interacting with one or more of these bases.


DNA Staining: Definition, Procedures, Benefits, Dyes and Uses

DNA Staining: Definition, Procedures, Benefits, Dyes and Uses

2024-10-26

DNA staining is a very important molecular biology technique that is used in genetics, cell biology, and molecular diagnostics. Its fundamental premise is to use dyes which attach to molecules of DNA to capture DNA, and so reveal DNA under the microscope or with fluorescent signals. Using staining, scientists can then see how the DNA has organised in the cell nucleus, perform genetic testing, or monitor the abundance and purity of DNA present in samples. As molecular biology technologies become better suited for different experimental and use cases, DNA staining procedures get tweaked.


Cell Staining: Definition, Principles, Protocols, Dyes, and Uses

Cell Staining: Definition, Principles, Protocols, Dyes, and Uses

2024-10-20

The growth, morphology and even biological features of the cells should be monitored once they are cultured. Because cells are so tiny and specialized, the appearance and shape of them, let alone the molecular nature and role of many cell components, is difficult to see without the right techniques. There are now many ways to study cells – from optical microscopes to electron microscopes, general cytochemical to immunochemical. The cell staining is a very common approach to cellular biology in which certain dyes are applied to imprint and observe cells and the inner layers of the cells. It's an experimental method of recording the shape, size and function of cells that is useful for studies of cell biology, pathology and development.


Ion Imaging: Definition, Principles, Benefits, Dyes, and Uses

Ion Imaging: Definition, Principles, Benefits, Dyes, and Uses

2024-10-16

Inorganic ions within organisms are essential substances that play vital roles in life processes. Increasing research indicates that disruptions in ion balance are closely associated with health issues, with most disease development processes accompanied by inorganic ion disorders. This creates a microenvironment with ion discrepancies at disease sites. Thus, evaluating ion levels in vivo and monitoring their dynamic changes is particularly important for the early, precise diagnosis and treatment of diseases, as well as for exploring disease mechanisms.


Fluorescent Labeling: Definition, Principles, Types and Applications

Fluorescent Labeling: Definition, Principles, Types and Applications

2024-10-07

Fluorescence labeling technology originated in the 1940s, when fluorescently labeled antibodies were used to detect some corresponding antigens. With the development of molecular biology, modern medicine, and the application of various advanced fluorescence detection technologies and instruments, fluorescence labeling as a new, non-radioactive labeling technology has received great attention and has achieved extremely rapid development. It has been widely used in the detection of substances inside and outside cells, nucleic acid detection, and early diagnosis of diseases, and has played an important role in the fields of biological research and medical research.

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