Technical Articles
DNA Staining: Definition, Procedures, Benefits, Dyes and Uses
2024-10-26
DNA staining is a very important molecular biology technique that is used in genetics, cell biology, and molecular diagnostics. Its fundamental premise is to use dyes which attach to molecules of DNA to capture DNA, and so reveal DNA under the microscope or with fluorescent signals. Using staining, scientists can then see how the DNA has organised in the cell nucleus, perform genetic testing, or monitor the abundance and purity of DNA present in samples. As molecular biology technologies become better suited for different experimental and use cases, DNA staining procedures get tweaked.
Cell Staining: Definition, Principles, Protocols, Dyes, and Uses
2024-10-20
The growth, morphology and even biological features of the cells should be monitored once they are cultured. Because cells are so tiny and specialized, the appearance and shape of them, let alone the molecular nature and role of many cell components, is difficult to see without the right techniques. There are now many ways to study cells – from optical microscopes to electron microscopes, general cytochemical to immunochemical. The cell staining is a very common approach to cellular biology in which certain dyes are applied to imprint and observe cells and the inner layers of the cells. It's an experimental method of recording the shape, size and function of cells that is useful for studies of cell biology, pathology and development.
Ion Imaging: Definition, Principles, Benefits, Dyes, and Uses
2024-10-16
Inorganic ions within organisms are essential substances that play vital roles in life processes. Increasing research indicates that disruptions in ion balance are closely associated with health issues, with most disease development processes accompanied by inorganic ion disorders. This creates a microenvironment with ion discrepancies at disease sites. Thus, evaluating ion levels in vivo and monitoring their dynamic changes is particularly important for the early, precise diagnosis and treatment of diseases, as well as for exploring disease mechanisms.
Fluorescent Labeling: Definition, Principles, Types and Applications
2024-10-07
Fluorescence labeling technology originated in the 1940s, when fluorescently labeled antibodies were used to detect some corresponding antigens. With the development of molecular biology, modern medicine, and the application of various advanced fluorescence detection technologies and instruments, fluorescence labeling as a new, non-radioactive labeling technology has received great attention and has achieved extremely rapid development. It has been widely used in the detection of substances inside and outside cells, nucleic acid detection, and early diagnosis of diseases, and has played an important role in the fields of biological research and medical research.
