Fluorescence Imaging
BOC Sciences specializes in the custom synthesis and production of fluorescent dyes and probes, catering to the diverse and precise application needs of fluorescence imaging technology. We provide end-to-end services from molecular design and synthesis to large-scale production, ensuring high sensitivity, excellent photostability, and specificity of the products. Relying on our advanced organic synthesis platform and experienced R&D team, we can customize fluorescent dyes and probes with tailored wavelengths, spectral properties, and functional modifications according to customer requirements, including pH-sensitive, ion-responsive, and biomolecule-targeting functionalities.
Fluorescence Imaging Technology
Fluorescence imaging is a technique that uses the phenomenon of fluorescence emitted by fluorescent substances when excited by light of specific wavelengths to observe and record biological samples. This technology is widely applied in fields such as cell biology, molecular biology, drug screening, and clinical diagnostics. The core of fluorescence imaging lies in fluorescent dyes and probes, which can specifically label target molecules or structures, providing clear images under a microscope. The selection of fluorescent dyes and probes is crucial to the imaging results. Ideal fluorescent dyes should possess high brightness, good photostability, suitable excitation and emission wavelengths, and low toxicity. Additionally, probes with specific functions, such as pH-sensitive probes, ion probes, and reactive oxygen species probes, need to be developed according to different research requirements.
Why Choose Our Fluorescent Reagent Development Services?
Custom Spectral
CharacteristicsBased on customer needs, we adjust the excitation and emission wavelengths of fluorescent dyes, covering a full spectral range from ultraviolet to near-infrared to accommodate multi-color fluorescence imaging and multiplex labeling experiments.
Functional Modification Customization
We provide various functional modifications for specific application scenarios, such as hydrophilization, hydrophobization, bioconjugation, and targeted ligand modifications, to improve the biological compatibility, cellular permeability, and targeting ability of dyes.
Optimization of Physicochemical Properties
According to experimental requirements, we optimize the photostability, quantum yield, solubility, and photobleaching resistance of dyes to ensure efficient and stable performance in complex biological environments.
Support for Special Application Scenarios
For specialized imaging needs (e.g., super-resolution imaging, two-photon imaging, in vivo imaging), we offer custom development of high-brightness and high-stability fluorescent dyes.
High-End Fluorescent Dyes Covering Your Imaging Needs in Multiple Dimensions
BOC Sciences provides customized fluorescent dye services, designing and synthesizing fluorescent dyes with specific spectral characteristics, solubility, and stability based on customer needs. Through personalized solutions and precise technical services, BOC Sciences supports scientific research and industrial innovation, driving breakthrough advancements in fluorescence imaging technology.
Organic Fluorescent Dyes
- Rhodamine
- Coumarin
- Alexa Fluor
- BODIPY
- Cyanine
- Carbazole Dyes
Fluorescent Protein Dyes
- Green Fluorescent Protein (GFP)
- Red Fluorescent Protein (RFP)
- Blue Fluorescent Protein (BFP)
- Yellow Fluorescent Protein (YFP)
- Near-Infrared Fluorescent Protein (NIR FP)
- pH-Sensitive Fluorescent Protein
Near-Infrared Fluorescent Dyes
Fluorescent Probe Technology Innovation: From Molecular Design to Application Breakthroughs
BOC Sciences also offers custom fluorescent probe services, designing and synthesizing probes with specific targeting, responsiveness, and spectral properties according to the customer's research goals. We have an experienced R&D team capable of designing and synthesizing fluorescent probes with specific spectral characteristics (absorption and emission wavelengths), high sensitivity, and selectivity, based on customers' specific application needs, such as organelle labeling, protein detection, and active molecule tracing.
Ion Fluorescent Probes
- Calcium Ion Probes (Fura-2, Fluo-3)
- Chloride Ion Probes
- Sodium Ion Probes
- Magnesium Ion Probes
- pH Probes (BCECF, SNARF)
ROS Fluorescent Probes
- DCFH-DA
- MitoSOX
- Hydroxyphenyl fluorescein (HPF)
- Aminophenyl fluorescein (APF)
- MitoPY1
Enzyme Activity Fluorescent Probes
- Caspase Probes
- MMP Probes
- Protease Probes
- Esterase Probes
- Phosphatase Probes
Tailored Fluorescent Functional Modification Services for Your Research
BOC Sciences has extensive chemical modification experience and advanced synthesis platforms, providing functional group modification, targeting group introduction, light stability optimization, and solubility regulation of fluorescent dyes according to customer needs. Our team excels at customizing high-performance fluorescent probes suitable for biological labeling, imaging analysis, and diagnostic testing, ensuring high sensitivity and selectivity.
Design and Synthesis
Design fluorescent dyes with specific optical properties, such as excitation/emission wavelengths, quantum yield, and light stability. Use advanced synthesis techniques to prepare high-purity, high-quality fluorescent dyes.
Structural Modification and Functionalization
Modify the structure of fluorescent dyes and probes according to customer needs, such as introducing functional groups like biotin, acyl halides, azides, etc., to achieve specific labeling or conjugation reactions.
Targeting Modification
Conjugate targeting molecules (such as antibodies, peptides, or nucleic acids) with fluorescent dyes to enable highly specific detection of particular biomolecules.
Environmental Response Modification
Develop fluorescent dyes and probes sensitive to environmental changes such as pH, redox potential, temperature, etc.
Wavelength Regulation and Optimization
Adjust the absorption and emission wavelengths of fluorescent dyes and probes according to experimental needs, enabling multi-color imaging and high-resolution detection.
Formula Optimization and Packaging Services
Provide different packaging forms, such as powder or solution, and optimize concentration and solvent based on experimental requirements to ensure the stability and effectiveness of dyes and probes in practical applications.
One-Stop Fluorescent Imaging Kit Development Service
To meet various research needs, BOC Sciences has also developed a series of fluorescent imaging kits. These kits contain essential components such as fluorescent dyes, probes, and buffers, allowing users to conduct fluorescent imaging experiments simply by following the instructions. BOC Sciences’ fluorescent imaging kits are easy to use, reliable, and offer good reproducibility, making them widely applicable in fields like cell imaging, tissue imaging, and in vivo imaging.
Strict Quality Control Process to Ensure Your Research Success
BOC Sciences has established a strict quality control system throughout the development and production of fluorescent reagents, covering every stage from raw material selection to final product release, ensuring high performance and batch consistency of reagents.
- Raw Material Traceability: All chemical raw materials are sourced from ISO-certified suppliers and verified for purity (≥98%) using NMR (nuclear magnetic resonance) and MS (mass spectrometry).
- Biomolecule Verification: Antibodies, nucleic acids, and other biological materials undergo SDS-PAGE, HPLC, or ELISA testing to ensure activity and specificity.
- Real-Time Analysis: During fluorescent dye synthesis, online UV-Vis and fluorescence spectroscopy are used to track reaction progress, optimizing yield and impurity control.
- Purity and Stability: Dye purity (>95%) is evaluated using HPLC and LC-MS (liquid chromatography-mass spectrometry), and accelerated aging experiments verify storage stability (≥2 years at -20 °C).
- Fluorescent Performance: Measure quantum yield (Φ), Stokes shift, and photobleaching resistance (e.g., Cy3 maintains >80% fluorescence intensity after 1 hour of laser exposure).
- Biocompatibility: Ensure reagent safety through cytotoxicity tests (MTT method) and hemolysis tests (hemoglobin release<5%).
- Application Scenario Simulation: Verify fluorescent stability and background signal in customer-specified buffers (e.g., PBS, cell culture media).
Advanced Analytical Platform
- UV-Vis Spectrophotometer
- FTIR
- NMR
- Fluorescence Spectroscopy
- HPLC
- GC
- GPC
- TLC
- LC-MS
- GC-MS
- AAS
- ICP-MS
- XRF
- DSC
- TGA
- Melting Point Apparatus
- Polarimeter
- Viscometer
- XRD
- Karl Fischer Titration
What Molecular Imaging Solutions Can We Provide for Your Study?
Cell Imaging
Fluorescent dyes and probes can specifically label certain structures or molecules within the cell, such as the nucleus, mitochondria, and endoplasmic reticulum. Using fluorescence microscopy, researchers can monitor real-time changes in cell morphology, molecular localization, and dynamic processes.
Tissue Imaging
At the tissue level, fluorescent dyes and probes can label specific cell types or tissue structures, such as tumor cells, blood vessels, and nerve fibers. Through fluorescence imaging, researchers can study physiological and pathological processes in tissues, such as tumor growth, angiogenesis, and nerve regeneration.
In Vivo Imaging
Near-infrared fluorescent dyes and probes have important applications in in vivo imaging. By injecting fluorescent probes into animals, researchers can observe molecular processes in live organisms in real-time, such as drug distribution, tumor metastasis, and inflammatory responses. In vivo imaging technologies provide powerful tools for drug development and disease diagnosis.
Molecular Interaction Studies
Fluorescence Resonance Energy Transfer (FRET) technology uses fluorescent dyes and probes to study molecular interactions. By labeling two interacting molecules, researchers can monitor their binding and dissociation processes in real-time, revealing molecular mechanisms.
Molecular Diagnostics
Fluorescent reagents are widely used in molecular diagnostics for nucleic acid testing and protein labeling, helping to accurately detect disease-related biomarkers. By specifically binding to target molecules, fluorescent probes enable high-sensitivity and high-specificity quantitative analysis, such as PCR amplification detection, FISH (Fluorescence In Situ Hybridization), and immunofluorescence analysis.
Drug Screening
In drug screening, fluorescent reagents are used in high-throughput screening (HTS) and high-content imaging (HCI). By labeling target proteins, signaling pathways, or organelles, researchers can monitor the interactions between drugs and targets in real-time. The application of fluorescent reporter genes and fluorescent probes makes the screening process more efficient, sensitive, and capable of quantitatively assessing drug activity, toxicity, and mechanisms, accelerating new drug development.
Environmental Monitoring
Fluorescent reagents are used in environmental monitoring to detect pollutants, heavy metal ions, and organic compounds. By utilizing the high selectivity of fluorescent dyes or probes to recognize and respond to target substances, researchers can monitor changes in the concentration of trace pollutants in the environment in real-time. With high sensitivity and rapid response advantages, these technologies are widely applied in water quality detection, air pollution monitoring, and food safety analysis.
FAQs About Fluorescence Imaging
What is fluorescence imaging?
Fluorescence imaging is a technique that uses the property of fluorescent substances to emit light when exposed to light of specific wavelengths, enabling the observation and analysis of biological samples or materials. Fluorescent substances (such as fluorescent dyes or proteins) absorb short-wavelength light (e.g., ultraviolet or blue light) and then emit light at longer wavelengths (e.g., green or red light). This technology is widely used in fields like biology, medicine, and materials science to study cell structures, molecular interactions, and disease diagnosis. Fluorescence imaging features high sensitivity and resolution, allowing real-time dynamic observation of biological processes in living samples.
How does fluorescence imaging work?
Fluorescence imaging works based on the properties of fluorescent substances. First, the fluorescent material is excited by light of a specific wavelength, absorbing energy and entering an excited state. Subsequently, when the fluorescent substance returns to its ground state, it releases energy in the form of light at a longer wavelength, which is fluorescence. Fluorescence microscopes or other imaging devices capture these fluorescence signals and form images. Key steps in fluorescence imaging include selecting appropriate fluorescent markers, optimizing excitation light sources and filter systems, and using highly sensitive detectors to capture faint fluorescence signals.
What are the benefits of fluorescence imaging?
Fluorescence imaging offers several advantages. Firstly, it has high sensitivity and resolution, capable of detecting very low concentrations of fluorescent markers and clearly displaying microscopic structures. Additionally, fluorescence imaging allows for multicolor labeling, enabling the simultaneous observation of multiple target molecules or structures, providing rich information. Moreover, fluorescence imaging causes minimal damage to living samples, making it suitable for long-term dynamic observations. In the medical field, fluorescence imaging can be used for early disease diagnosis and surgical navigation, enhancing treatment accuracy. Overall, fluorescence imaging is a powerful and versatile tool widely used in both research and clinical practice.
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