Fluorescence In Situ Hybridization (FISH)
In the fields of modern life sciences and precision medicine, fluorescence in situ hybridization (FISH) technology is widely applied in gene detection, chromosome analysis, tumor diagnosis, and personalized treatment due to its high sensitivity, high specificity, and visualization advantages. To meet the increasing demands of scientific research and applications for enhanced probe sensitivity, stability, and multiplex labeling, BOC Sciences leverages its robust capabilities in fluorescent dye synthesis, nucleic acid modification technologies, and labeling platforms to provide high-quality, customized fluorescent probes and dye solutions for FISH applications. Our product portfolio includes directly and indirectly labeled dyes, custom probes, antibody-fluorophore conjugates, nucleic acid stains, and various auxiliary reagents, supporting global customers in achieving more accurate, stable, and high-throughput FISH detection goals.
What is Fluorescence In Situ Hybridization?
Fluorescence in situ hybridization (FISH) is a highly sensitive molecular biology technique used to localize specific nucleic acid sequences within cells or tissue samples. Its basic principle involves hybridizing a fluorescently labeled probe complementary to the denatured DNA or RNA target sequence in the sample, followed by fluorescence microscopy observation of the location and distribution of specific sequences. FISH enables visualization and analysis of genetic information such as genome structural variations, chromosomal rearrangements, gene amplification, and deletions through the intensity and localization of fluorescent signals. Compared with traditional chromosome analysis methods, FISH offers advantages of high sensitivity, strong specificity, precise localization, and the ability to perform multiplex detection. It can also detect gene status during interphase of mitosis, significantly expanding its application scenarios. FISH is widely used in genetic disease diagnosis, cancer subtyping, gene expression research, microbial classification, and reproductive medicine, making it an indispensable tool in basic research and clinical molecular diagnostics.
Advantages of BOC Sciences in Fluorescent Dye Solutions
Instrument Compatibility
BOC Sciences offers fluorescent dyes compatible with a variety of fluorescence analysis instruments, ensuring matched excitation sources, filters, and sensitivity, thereby enhancing the reliability of experimental results.Optimized Absorption and Emission Spectra
Our fluorescent dyes feature precise absorption and emission spectra, perfectly aligned with different detection methods to ensure optimal signal output in FISH experiments.Wide Stokes Shift
We provide fluorophores with large Stokes shifts to avoid signal overlap among different fluorescent labels, especially in multiplex applications, ensuring clear results.Stable pH Sensitivity
Our fluorescent dyes exhibit excellent stability under various pH conditions, ensuring consistent fluorescence signals in complex FISH experiments regardless of environmental changes.
Fluorescent Reagent Solutions for FISH by BOC Sciences
In FISH experiments, the choice of fluorescent dyes and auxiliary reagents directly affects the sensitivity, specificity, and clarity of hybridization signals. BOC Sciences is committed to providing high-performance, customizable FISH fluorescent reagent solutions for research institutions and diagnostic laboratories, covering the entire workflow from probe-labeling dyes to auxiliary detection reagents. We understand the stringent requirements for reagent purity, fluorescence stability, and signal-to-noise ratio in FISH experiments, and we continuously optimize our product systems to meet the diverse experimental needs from single gene detection to multiplex hybridization, from cell samples to tissue sections. BOC Sciences offers a wide range of reagents and flexible labeling options to meet customer needs for sensitivity, stability, and quantitative analysis across various applications, supporting researchers in obtaining high-resolution and highly reproducible hybridization signals.
Fluorescent Dyes for Directly Labeled Probes
We offer a variety of high-brightness and photostable fluorescent dyes (such as Cy3, FITC, Alexa Fluor series) suitable for directly labeling oligonucleotide probes. These support 5’-end, 3’-end, or internal modifications to optimize hybridization efficiency and spatial conformation.
Antibody-Fluorophore Conjugates for Indirect Labeling Systems
For probes labeled with biotin, digoxigenin, and others, we provide a range of matched fluorescent antibody conjugates, including monoclonal/polyclonal antibody options conjugated with various dyes, enhancing signal amplification.
Nucleic Acid Dyes and Background Suppressors
For nuclear staining and background contrast, we offer classical nucleic acid dyes such as DAPI and Hoechst to assist in signal localization and image structure recognition. Auxiliary reagents including hybridization buffers, washing solutions, and quenchers are also provided to optimize hybridization and visualization conditions.
Dyes for Multiplex Probes
Our multiplex dye solutions combine fluorescent dyes with different emission wavelengths, including single-color, dual-color, and multicolor FISH probes, enabling simultaneous detection of multiple targets and multichannel image acquisition.
One-Stop FISH Probe Design and Labeling Services by BOC Sciences
BOC Sciences offers various types of FISH probes, including oligonucleotide probes, DNA fragment probes, RNA probes, and LNA-modified probes, to meet diverse experimental requirements. We provide customized design and synthesis services based on customer specifications to ensure probes possess excellent specificity, stability, and high sensitivity, suitable for various gene localization and analysis applications. Whether for single-target or multiplex detection projects, we deliver precise probe design guidance, flexible structure optimization suggestions, and suitable labeling strategies. Through personalized adjustments in probe length, modification positions, and fluorophore types, we can effectively improve hybridization efficiency and image resolution. In addition, our services include probe purification, concentration preparation, and multiplex probe mixing, helping customers save time, reduce reagent waste, and ensure experimental reproducibility—making us a trusted partner in both basic research and clinical applications.
Fluorescent Probe Design
We offer professional sequence analysis and structural design services based on target sequence characteristics and experimental needs, ensuring probes with high specificity and excellent hybridization performance. Through strict selection, the designed probes can effectively avoid non-specific binding, thus ensuring experimental accuracy and reliability.
Probe Synthesis and Labeling Modification
BOC Sciences offers multiple chemical modification options and supports high-purity probe synthesis. We introduce functional tags such as fluorophores, biotin, or digoxigenin according to customer requirements. These modifications enhance probe stability, sensitivity, and detectability to meet different experimental demands.
Probe Length and Structure Optimization
We tailor the length and structure of probes according to the specific needs of experiments. Whether single-stranded, double-stranded, or LNA-modified probes, we customize them based on the characteristics of the target sequence to optimize stability, affinity, and signal strength.
Fluorescence Labeling Strategy Selection
To meet the requirements of fluorescence microscopy imaging, BOC Sciences recommends appropriate dye types and labeling sites based on specific experimental needs. We optimize dye selection to ensure signal intensity and stability, while improving multiplex detection compatibility and signal-to-noise ratio.
Quality Control Services for FISH Probes by BOC Sciences
BOC Sciences understands that every research and clinical test requires high-quality and reliable probes. Therefore, we implement strict quality control procedures throughout the probe synthesis, labeling, and application processes. We are committed to delivering thoroughly tested and validated FISH probes to ensure stable performance, high signal-to-noise ratio, and strong specificity under various complex experimental conditions. Through an efficient quality control system, we not only effectively reduce the risk of experimental failure but also improve the reproducibility and accuracy of experimental results.
- Purity Testing: Using HPLC and mass spectrometry to ensure the chemical purity and molecular weight of the probes meet standard requirements.
- Sequence Accuracy Testing: Employing sequencing technology to verify probe sequences, ensuring complete consistency with the target sequences.
- Fluorescent Labeling Efficiency Evaluation: Assessing the coupling efficiency of fluorescent labeling to ensure uniform fluorescence signals in each probe.
- Stability Testing: Conducting long-term storage tests to evaluate the stability and durability of probes under different environmental conditions.
- Hybridization Efficiency Testing: Performing preliminary hybridization experiments to assess the hybridization performance and signal intensity of the probes under specific experimental conditions.
Advanced Detection Platforms
- Fluorescence Microscope
- Laser Scanning Confocal Microscope
- Spectrophotometer
- High-Performance Liquid Chromatography (HPLC)
- Mass Spectrometer
- Polyacrylamide Gel Electrophoresis (PAGE)
- Liquid Chromatography-Mass Spectrometry (LC-MS)
- Fluorescence Spectrometer
- High-Performance Liquid Chromatography with Fluorescence Detection
- Electron Microscope
- Temperature Control System
- Real-time PCR Instrument
Extensive Applications of FISH in Research and Diagnostics
The FISH fluorescent dyes and probe solutions provided by BOC Sciences play a critical role in a wide range of scientific and clinical applications. With advanced probe synthesis and fluorescence labeling technologies, our products are widely used in genomics, genetics, cancer research, microbiology, and other fields. FISH technology enables precise visualization of molecular information in samples through fluorescence signal localization and intensity, helping scientists better understand gene functions, structural changes, and disease mechanisms. Below are typical applications of our solutions across major fields:
Genetic Research
FISH is widely used in chromosome localization and variation analysis. By detecting gene rearrangements, deletions, and amplifications, it helps researchers identify potential causes of hereditary diseases. BOC Sciences' FISH dyes and probes can accurately localize specific gene regions, enhancing the accuracy and reliability of genetic studies.
Cancer Research and Typing
In cancer diagnosis and typing, FISH can detect specific gene amplifications or deletions in cancer cells, aiding in early diagnosis and precise classification. Our fluorescently labeled probe system supports multiplex gene analysis, providing a powerful tool for cancer research and advancing personalized treatment.
Microbial Classification and Detection
FISH is also widely used in microbiology, particularly in the classification, localization, and quantitative analysis of microorganisms. By using specific probes, BOC Sciences' fluorescent dyes help researchers accurately detect bacteria and viruses in environmental or clinical samples, supporting rapid diagnosis of infectious diseases.
Gene Expression Analysis
FISH can be used to locate the expression of specific genes in cells or tissues. RNA probes provided by BOC Sciences effectively identify and locate mRNA molecules, helping researchers understand gene expression levels under different physiological or pathological conditions and advancing transcriptomics research.
Clinical Diagnosis
In clinical diagnostics, FISH technology has been applied to detect hereditary diseases, chromosomal abnormalities, and tumor marker expression. The FISH reagent solutions provided by BOC Sciences support precise labeling and multiplex hybridization applications, assisting physicians in early disease warning, diagnosis, and precision treatment.
FAQs About Fluorescence In Situ Hybridization (FISH)
How does fluorescence in situ hybridization work?
Fluorescence in situ hybridization (FISH) is a molecular cytogenetic technique used to detect the location of specific DNA or RNA sequences in cell or tissue samples. Its principle is to hybridize fluorescently labeled nucleic acid probes with target nucleic acid sequences under in situ conditions. After hybridization, the binding site of the probe and the target sequence emits fluorescence signals observed through a fluorescence microscope, thereby visualizing specific genes or chromosomal regions. FISH is widely used in research and clinical fields due to its simplicity, high sensitivity, and accurate spatial localization.
What can fluorescent in situ hybridization detect?
FISH can detect various genetic information at the nucleic acid level, including chromosomal structural abnormalities (such as deletions, duplications, inversions, and translocations), specific gene amplifications or deletions, gene rearrangements, RNA expression localization, and the presence of viral DNA. It is applicable in analyzing genetic diseases, gene abnormalities in cancer, preimplantation genetic screening, and identification and distribution of microorganisms. With high specificity and sensitivity, FISH has become an important tool in studying nucleic acid variation and molecular mechanisms in cells.
What is fluorescent in situ hybridization used for?
FISH is widely used in both basic research and clinical diagnostics. In medicine, it is commonly used for cancer diagnosis (e.g., HER2 gene amplification detection), genetic disease screening, preimplantation gene analysis, and chromosomal abnormality analysis. In basic scientific research, FISH is used for gene localization, gene expression pattern studies, cell development tracing, and microbial community analysis. Its ability to visualize specific nucleic acid sequences makes it a vital tool for revealing gene functions, expression regulation, and mechanisms of cell differentiation.
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