Fluorescent Protein Services
In modern life science research and biotechnology development, fluorescent proteins (FPs) have become essential molecular tools. They are genetically expressed within cells, providing stable and efficient fluorescence signals. FPs are widely used in various fields such as cell imaging, protein labeling, gene expression analysis, in vivo tracking, and high-throughput screening. Leveraging its strong expertise in molecular biology and protein engineering, BOC Sciences offers comprehensive and customized fluorescent protein services for global research and industrial clients, aiming to provide precise and high-performance solutions for life science research, drug development, and diagnostic applications.
Why Choose Fluorescent Proteins?
Genetic Expression with Stable Labeling
Fluorescent proteins can be genetically expressed within cells, enabling long-lasting and inheritable labeling, suitable for long-term tracking and dynamic studies.
No Exogenous Dyes Required, High Biocompatibility
Without the need for external dyes or substrates, FPs are applicable to live-cell and in vivo experiments, reducing interference and enhancing experimental safety and stability.
Multicolor Options for Multi-Channel Imaging
Available in a wide range of chromophores from violet to near-infrared, supporting dual-, triple-labeling and FRET experiments, ideal for complex multifactorial imaging analysis.
Broad Applications with Flexible Functionality
Applicable for protein localization, signal pathway monitoring, reporter gene systems, and biosensor development, offering diverse functions and research utilities.
What Fluorescent Proteins Do We Offer?
BOC Sciences has established a comprehensive and structurally diverse fluorescent protein technology platform, supporting multi-wavelength, multi-purpose, and multi-expression system-based protein screening and custom development. Our fluorescent proteins span from violet to near-infrared spectral ranges. Combined with ongoing optimizations in structural engineering, expression efficiency, and functional coupling, our platform meets the diverse needs of both academic and industrial clients.
Green Fluorescent Protein
GFP is one of the earliest and most widely used commercial fluorescent proteins, originally derived from the jellyfish Aequorea victoria, known for its excellent photostability and high brightness. We offer multiple GFP variants to suit different application needs:
- EGFP (Enhanced Green Fluorescent Protein)
- sfGFP (Superfolder GFP, suitable for fusion expression)
- cpGFP (Circularly Permuted GFP, used in biosensor development)
Cyan Fluorescent Protein
CFP has a shorter excitation wavelength and an emission range of 470–490 nm, often used in FRET experiments and multi-channel imaging. Representative proteins include:
- ECFP (Enhanced Cyan Fluorescent Protein)
- mCerulean (an optimized version of Cerulean with improved brightness and photostability)
- mTurquoise (especially mTurquoise2, known for outstanding brightness and stability)
Red Fluorescent Protein
RFPs exhibit excellent cellular penetration and photostability, suitable for cellular and tissue imaging. Common variants include:
- DsRed (tetrameric)
- mCherry, mRFP1, mKate2, mCardinal (monomeric, bright and stable)
- tdTomato (dimeric, high brightness, ideal for tissue imaging)
Blue Fluorescent Protein
BFPs are engineered via mutations from the GFP family, with excitation wavelengths of 380–410 nm and emissions around 440–470 nm, suitable for high-energy blue light imaging:
- mTagBFP, EBFP (high-energy blue light sources)
- iRFP, mNeptune, mIFP (used for deep tissue/small animal imaging)
Yellow Fluorescent Protein
YFP emits at 525–540 nm and is commonly used for live-cell imaging and FRET experiments. Representative variants include:
- Venus (low pH sensitivity)
- Citrine (good photostability, suitable for long-term imaging)
- YPet (high brightness and quantum efficiency)
- YFP-H148D (improved pH stability and fluorescence efficiency)
Looking for Expert Guidance?
BOC Sciences leverages advanced fluorescence technologies to propel your research forward, enabling next-generation breakthroughs in drug discovery and diagnostics.
Custom Fluorescent Protein Design and Expression Services
To meet the growing demand for diverse and high-performance fluorescent proteins in research and industrial applications, BOC Sciences provides expert design and expression services. Our offerings include construction of customized expression vectors, precise fusion protein design, and efficient protein production and purification using various expression systems. We ensure optimal support and reliable outcomes for clients in experimental design and application development. Whether for basic research or complex functional protein expression, we deliver flexible, professional, and high-quality solutions to accelerate scientific innovation and industrial advancement.
Custom Expression Vectors and Fusion Protein Design
BOC Sciences provides flexible gene cloning and vector construction services. We support client-specified promoters, tags, antibiotic resistance genes, and plasmid backbones, and can also recommend standard options to ensure expression efficiency and experimental compatibility. Customization includes:
- N- or C-terminal fusion of fluorescent protein with target protein;
- Multi-tag fusion (e.g., His-tag + GFP);
- Tissue-specific expression vector design;
- Inducible expression systems (Tet-On, Tet-Off);
- Special linker design (flexible linker, anti-interference linker);
- Constructs containing signal peptides, nuclear localization signals, or membrane localization signals.
Fluorescent Protein Expression and Purification Services
BOC Sciences provides end-to-end expression and purification services, from small-scale trials to large-scale production, supporting various expression systems. Delivery formats include crude lysates, purified proteins, lyophilized powders, and buffer formulations.
- E. coli expression system (suitable for basic FPs);
- Mammalian cell expression system (for complex FPs or fusion proteins);
- Insect cell system (Baculovirus): for large fluorescent fusion proteins;
- Yeast system: for secreted fluorescent protein production;
- Lentiviral and adenoviral vector construction (for stable transfection/in vivo studies);
- Inducible expression and tissue-specific promoter systems.
Functional Fluorescent Protein Probe Development Services
In addition to serving as labels, fluorescent proteins can be engineered into functional probes that respond to environmental signals. BOC Sciences supports the development of intelligent fluorescent probes in the following categories:
| Probe Type | Function | Application Area |
| pH-sensitive FP (e.g., pHluorin) | Responds to intracellular/extracellular pH changes | Endocytosis and exocytosis studies |
| Calcium indicator proteins (e.g., GCaMP series) | Responds to calcium ion concentration | Neuronal activity imaging, signaling tracking |
| FRET/BRET probes | Analyze molecular distance and energy transfer | Protein interactions, kinase activity analysis |
| Photoactivatable fluorescent proteins (e.g., PA-GFP, Dronpa) | Fluorescence activation/deactivation via light | Optogenetics, cell tracking |
Competitive Service Advantages
- Comprehensive Fluorescent Protein Library: Covers full spectral range from violet to near-infrared, suitable for multiplex labeling, multi-channel imaging, and complex FRET designs.
- Multi-System Expression Capability: Supports E. coli, mammalian, yeast, and insect cell systems, adaptable to diverse protein types and research needs.
- Protein and Vector Customization: Offers fusion construction, tag design, signal regulation, and viral packaging to meet personalized research and industrial development requirements.
- Functional Validation and Application Assessment: Includes functional testing in cell and animal models to verify FP expression, localization, and biological activity.
- Advanced Functional Probe Development: Supports the design and development of FRET/BRET sensors, photoactivatable FPs, and pH or calcium-responsive probes.
- Integrated Workflow: Covers the entire process from gene design to protein validation, managed under a unified platform to save time and reduce experimental costs.
- Rapid Response Mechanism: Dedicated project managers ensure fast communication and smooth project progress.
- High-Quality Delivery: Strict quality control system with support for SDS-PAGE, LC-MS, and fluorescence validation to guarantee protein purity and functional reliability.
Fluorescent Protein Service Workflow
To ensure efficient execution and high-quality delivery of client projects, BOC Sciences has established a scientifically rigorous, standardized, and modular service workflow that covers the full lifecycle from project initiation, program design, experimental execution, to quality control and final delivery.
Requirement Communication
Initial communication with the client is conducted to thoroughly understand the project background and experimental objectives. This includes clarifying the type of fluorescent protein (e.g., GFP, RFP, CFP), fusion method (N-terminal or C-terminal fusion, linker design, etc.), preferred expression system (E. coli, mammalian cells, yeast, insect cells), delivery format (plasmid DNA, expressed protein, lyophilized powder, buffer solution), and any special project requirements.
Program Design
Based on client requirements, the BOC Sciences scientific team will formulate a detailed technical strategy, including gene design for the fluorescent protein, vector construction plan, fusion protein structural model, recommended expression system, and quality control checkpoints. The project timeline, delivery specifications, and budget will be defined and compiled into a written proposal for client confirmation to ensure scientific soundness, practicality, and feasibility.
Gene Synthesis and Construction
Codon optimization and sequence streamlining strategies are applied to fully synthesize the target fluorescent protein and fusion genes. Vector construction is performed using high-fidelity enzyme cloning techniques, including promoter selection, tag configuration, and restriction site design. All constructed plasmids are verified by Sanger sequencing to ensure sequence accuracy and stable downstream expression.
Expression and Purification
The most suitable expression system is selected based on protein characteristics, and expression conditions (inducer concentration, temperature, duration, etc.) are established. Small-scale expression evaluation is followed by large-scale production. Target proteins are purified using affinity chromatography, ion exchange, gel filtration, and other methods. Purified samples undergo comprehensive quality control testing to ensure protein purity, concentration, and fluorescence performance meet client expectations. Customized buffer formulations and stabilizer treatments are available upon request.
Quality Control and Delivery
Each product batch is delivered with a detailed quality control report, including SDS-PAGE analysis, Western blot verification (e.g., tag detection), excitation/emission spectra, fluorescence intensity, and stability tests. Optional additional tests are available, such as LC-MS, endotoxin detection, and activity validation. Samples can be delivered in various formats to facilitate immediate use or long-term storage.
Technical Support
Following delivery, BOC Sciences provides ongoing technical support, including experimental optimization advice, troubleshooting, mutation services, or suggestions for extended applications. If clients encounter issues during actual usage, our technical team will respond quickly to resolve challenges and ensure product functionality in real applications.
Typical Applications of Fluorescent Proteins
As genetically encoded molecular probes, fluorescent proteins offer visualization, non-invasiveness, and high sensitivity, making them indispensable across numerous research and industrial domains. Through continuously optimized fluorescent variants and flexible customization services, BOC Sciences actively supports client applications in basic research, clinical translation, drug development, and diagnostic technologies.
Cell Imaging and Subcellular Localization
BOC Sciences provides multicolor monomeric fluorescent proteins and fusion constructs to precisely label subcellular structures such as nuclei, mitochondria, ER, and lysosomes. These are widely used for live-cell imaging and dynamic observation. We support multi-channel confocal microscopy, high-resolution imaging, and stable cell line construction, helping researchers track protein localization, organelle changes, and cellular behaviors in real-time, providing visual analysis tools for fields such as cell biology and pharmacology.
Gene Expression Regulation and Transcriptional Activity Monitoring
Fluorescent proteins are frequently used as reporter genes for promoter activity assays, transcription factor regulation studies, and gene editing system validation (e.g., CRISPR). BOC Sciences offers customized reporter system design, including dual-fluorescence, inducible expression, and tissue-specific vectors, enabling efficient assessment of exogenous gene expression levels, RNAi efficiency, and transcriptional regulation dynamics, widely used in basic research, synthetic biology, and drug screening platforms.
Drug Screening and High-Throughput Analysis Platforms
In drug discovery and functional screening, fluorescent proteins often serve as signal output modules for rapid evaluation of drug effects, toxicity, and activity pathways. BOC Sciences supports construction of screening models based on fluorescent signal responses, including cytotoxicity detection, protein-protein interaction analysis, signaling pathway responses, and CRISPR library screening. These systems, coupled with high-throughput equipment and quantitative imaging solutions, provide clients with sensitive, stable, and scalable tools for drug screening.
In Vivo Imaging and Animal Experimentation
For tissue imaging and in vivo tracing needs, BOC Sciences offers a variety of red and near-infrared fluorescent proteins suitable for small animal imaging, tissue distribution monitoring, and cell migration tracking. We construct specific expression vectors or viral systems to label and trace stem cells, immune cells, and tumor cells. Combined with imaging equipment, this enables long-term, deep-tissue, and high signal-to-noise ratio fluorescence observation, supporting translational research and disease mechanism studies.
Protein Interaction and Signaling Pathway Studies
BOC Sciences provides FRET/BRET fluorescent protein pairs and corresponding fusion constructs for real-time visualization of molecular events such as protein-protein interactions, conformational changes, and kinase activity. Through donor-acceptor designs and dynamic imaging, clients can deeply analyze signaling pathways, receptor activation, and transduction mechanisms. We also support the construction of light-controlled fluorescent systems for spatial and temporal precision control of biomolecular functions, offering robust technical support for advanced molecular mechanism analysis.
Biosensing and Smart Materials
Due to their programmability, environmental responsiveness, and visualizability, fluorescent proteins are widely applied in the development of novel biosensors and smart materials. BOC Sciences can custom-develop pH-, Ca²⁺-, and ROS-responsive fluorescent proteins integrated into cellular sensors, nanoparticle probes, or material surfaces for real-time monitoring of microenvironmental changes, cellular metabolic states, or pathological processes. Additionally, we support fusing fluorescent proteins into smart materials such as thermosensitive or hydrogel systems to enable reversible responses to light, heat, or chemical signals, providing functional core modules for bioelectronics, diagnostic platforms, and synthetic biology systems.
FAQs About Fluorescent Proteins
What are fluorescent proteins?
Fluorescent proteins are a class of proteins that emit fluorescence upon excitation with specific wavelengths of light and are widely used in life science research. Originally derived from jellyfish (e.g., GFP), they can be genetically encoded and expressed in living cells or organisms to visualize protein localization, cellular dynamics, and signaling processes. FPs feature intrinsic chromophore formation, do not require external substrates, and can be stably inherited. Through engineering, they now span multiple color channels from blue to near-infrared. Today, they are core tools for cell imaging, molecular labeling, in vivo tracking, and biosensing.
What types of fluorescent proteins do you offer?
We offer a full-spectrum range from violet to near-infrared, including GFP, RFP, CFP, YFP, BFP, far-red fluorescent proteins, and engineered variants, suitable for multiplex imaging, FRET/BRET, in vivo tracking, and more.
Can fluorescent proteins be expressed in fusion with my target protein?
Yes. We provide customized fusion construction services, supporting N- or C-terminal fusions, linker design, signal peptide addition, and more to meet specific localization or functional analysis requirements.
Can you provide expression vectors? Are they customizable?
Yes, we can provide standard expression plasmids (e.g., for mammalian, bacterial, or viral systems) and support customization of promoters, tags, and selection markers to meet research or industrial-grade applications.
What expression systems do you support?
BOC Sciences supports a variety of expression systems including E. coli, mammalian cells, yeast, and insect cells, suitable for different protein types and functional needs, with expression scales ranging from micrograms to grams.
Can you provide purified fluorescent proteins? Are they functionally validated?
Yes, we provide high-purity protein samples, with SDS-PAGE purity analysis by default. Optional services include fluorescence spectroscopy, LC-MS identification, and functional validation to ensure quality control and functional reliability.
Can you develop functional fluorescent probes, such as calcium or pH sensors?
Yes. We have capabilities to design and express intelligent fluorescent proteins such as FRET/BRET probes, calcium sensors (e.g., GCaMP), and pH-sensitive proteins (e.g., pHluorin), customized according to experimental needs.
Can fluorescent proteins be expressed in animal models? Do you provide such services?
Yes. We support the construction of tissue-specific expression vectors suitable for models such as mice and zebrafish and can provide viral packaging services (e.g., lentivirus, AAV) for in vivo imaging and tracing applications.
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