Cell Staining

BOC Sciences operates as a top global supplier in chemical and biotechnology fields while providing numerous superior fluorescent dyes specifically designed for diverse cell staining applications. Our products deliver precise and dependable fluorescent labeling for nuclear staining of live, dead, or fixed cells, as well as specific staining of cellular organelles, cell membranes, and metabolic processes. Our fluorescent dyes find extensive application in experimental techniques including flow cytometry and confocal microscopy, along with live-cell imaging and cell viability assays, which bolster cell biology research.

What is Cell Staining?

The cell staining technique applies chemical or fluorescent dyes to mark cellular structures and specific molecules, allowing researchers to see cells and tissues more clearly under a microscope. Researchers in biology, medicine, and drug development use this technique extensively to study cell morphology and structure as well as cellular function. There are multiple cell staining techniques, which encompass nuclear staining, membrane staining, organelle staining, and specific molecular labeling. The use of cell staining extends to basic scientific research, including cell cycle analysis and apoptosis detection, as well as clinical applications like pathological diagnosis and cancer screening. Staining cells enables scientists to better comprehend cellular physiological and pathological processes, which leads to progress in disease treatment and drug development.

Transform Your Experiments with Superior Fluorescent Dye Technology

Advanced Synthetic Technology
We synthesize a wide range of fluorescent dyes, including small-molecule probes, NIR dyes, and protein labeling dyes.
Diverse Modification Capabilities
Our dyes offer flexible conjugation options with carboxyl, amine, thiol, and biotin modifications.
Innovative Fluorescent Probe Development
We develop novel probes for ROS detection, pH sensing, calcium ion indication, and environmental responsiveness.
High Sensitivity and Selectivity
Our dyes enable precise labeling with strong sensitivity, even at low concentrations.
Multiple Fluorescent Emission Wavelengths
We provide dyes with various emission wavelengths for multi-channel analysis.
Wide Application Compatibility
Our dyes are suitable for immunofluorescence, flow cytometry, and fluorescence microscopy.
Excellent Photostability and Light Resistance
Our dyes maintain brightness and signal stability, minimizing background interference.
High-Quality Assurance
We ensure strict quality control for high-purity, consistent fluorescent dyes.

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Fluorescent Dye Solutions to Support Various Cell Staining Techniques

BOC Sciences provides comprehensive cell staining support services, covering nuclear staining for live, dead, and fixed cells. We offer high-quality dyes for live-cell membranes and organelles, ensuring precise labeling of cellular structures. Additionally, our staining reagents are suitable for fixed cells and tissue sections, meeting the needs of immunofluorescence and histological analysis. For apoptosis and dead cell detection, we provide specialized staining solutions to support cell viability studies. We also supply flow cytometry cell cycle analysis reagents, facilitating research on cell proliferation and cycle progression. Moreover, our staining reagents are compatible with spheroids and 3D cultures, ensuring effective visualization in complex culture environments. BOC Sciences is committed to delivering efficient and highly sensitive cell staining solutions to support scientific research and drug development.

Organelle Staining Mitochondrial dyes Lysosomal dyes Endoplasmic reticulum dyes Golgi apparatus dyes Cytoskeleton dyes Cell membrane dyes
Live Cell Tracking Live-cell labeling Cell proliferation analysis Apoptosis detection Cell viability assessment
Ion and Redox State Detection Probes Calcium ion probes Sodium ion probes NO & ROS probes pH indicators

Exploring the Most Popular List of Cell Staining Dyes

The range of cell staining dyes offered by BOC Sciences is widely used in organelle labeling, live cell tracking, ion detection, and the detection of nitric oxide (NO) and reactive oxygen species (ROS). Organelle dyes specifically label different types of organelles, such as mitochondria, nuclei, and the endoplasmic reticulum, enabling researchers to observe changes in cellular structure in real-time. Live cell tracking dyes allow researchers to dynamically monitor cell migration and proliferation without compromising cell viability. Additionally, probes designed for detecting intracellular ion concentrations, as well as NO and ROS levels, provide powerful tools for studying cell metabolism, oxidative stress, and cell signaling. These dyes and probes offer extensive application potential in cell biology, drug screening, and disease mechanism research.

Dye Name	Application Area	Staining Principle/Features
DAPI	Nuclear Staining	Binds to DNA and emits blue fluorescence, widely used for nuclear staining in fixed cells and tissue sections.
Hoechst 33342	Nuclear Staining	Binds to DNA and emits blue fluorescence, commonly used for nuclear staining in live cells and for flow cytometry.
Propidium Iodide (PI)	Dead Cell Staining	Only penetrates dead cells, binds to DNA, and emits red fluorescence, commonly used for cell viability analysis.
FITC	Protein Labeling, Immunofluorescence	Binds to antibodies or other molecules, emits green fluorescence, commonly used in flow cytometry and immunohistochemistry.
Alexa Fluor	Immunostaining, Cell Labeling	Provides a variety of fluorescence colors, widely used in various fluorescence microscopy experiments, suitable for confocal and flow cytometry.
Rhodamine	Immunofluorescence, Membrane Labeling	Emits red fluorescence, commonly used for cell membrane and organelle labeling, suitable for confocal microscopy and flow cytometry.
Calcein-AM	Cell Viability Assay, Cytotoxicity Testing	Enters live cells and is converted to a fluorescent form, emitting green fluorescence, commonly used for cell viability and proliferation assays.
MitoTracker	Mitochondrial Labeling	Specifically labels mitochondria in live cells, emitting different colors of fluorescence. Commonly used in mitochondrial function studies and drug toxicity screening.
BODIPY	Lipid Labeling	Binds to lipids, emitting different colors of fluorescence, commonly used for cell membrane and lipid analysis, and lipid dynamics studies.
Alexa Fluor 647	Immunofluorescence, Cell Labeling	Suitable for flow cytometry and confocal microscopy, emitting red fluorescence, commonly used for protein labeling and cell surface markers.
Giemsa	Cell Staining (Especially Blood Cells)	Used for observing cell morphology, especially blood cell staining, commonly used in blood cell counting and leukemia research.
Coomassie Brilliant Blue	Protein Staining	Used for protein gel staining, especially in SDS-PAGE experiments. Suitable for detecting protein expression and analysis.
Sudan Black B	Lipid Staining	Specifically stains lipids in cells and tissues, commonly used in histology for lipid accumulation and metabolism studies.
Toluidine Blue	Tissue Section Staining	Commonly used for staining mucopolysaccharides and nucleic acids in tissue sections, emits blue fluorescence, suitable for fixed tissue sections.
MTT	Cell Proliferation, Cytotoxicity Testing	Used to assess cell proliferation and activity, converting into a purple precipitate, commonly used in cell proliferation and cytotoxicity assays.
Acridine Orange	Cell Nucleus and RNA Staining	Binds to both DNA and RNA, can distinguish live and dead cells. Commonly used for RNA localization and live cell staining.
Annexin V-FITC	Early Apoptotic Cell Staining	Used to detect early apoptotic cells by binding to externalized phosphatidylserine with FITC-labeled Annexin V.
Bromophenol Blue	Electrophoresis Gel Staining	Used for sample loading detection in electrophoresis, indicates pH changes, commonly used in protein and DNA electrophoresis.
Safranin O	Cell Wall Staining	Used for staining plant and bacterial cell walls, especially in plant biology and microbiology research.
Lugol's Iodine	Nuclear Staining, Membrane Staining	Mainly used for nuclear and cell membrane staining, especially for cell staining, plant cells, and microorganisms.

Advanced Quality Control Techniques Ensure Accuracy of Results

In terms of quality control, BOC Sciences employs multiple advanced technologies such as high-performance liquid chromatography (HPLC), mass spectrometry (MS), and ultraviolet-visible spectroscopy (UV-Vis) to comprehensively test the purity, concentration, molecular structure, and optical properties of the dyes. Additionally, we conduct photostability tests on the dyes to ensure they maintain efficient signal output during long-term experiments. To ensure batch-to-batch consistency and stability, we monitor every step of the production process in real time and optimize production workflows through data analysis.

UV-Visible Absorption Spectroscopy: Determines the absorption peaks and absorbance of the dye using UV-Visible spectroscopy to evaluate its optical properties.
Fluorescence Spectroscopy: Measures the fluorescence emission wavelength and intensity of the dye to assess its effectiveness in fluorescence staining applications.
Particle Size Distribution Measurement: Uses Dynamic Light Scattering (DLS) to measure the particle size distribution of the dye, ensuring uniformity and stability.
Chemical Structure Identification: Analyzes the molecular structure of the dye using Nuclear Magnetic Resonance (NMR) or Mass Spectrometry (MS) to confirm its purity and structural consistency.
Solubility Analysis: Evaluates the solubility of the dye in various solvents to determine its optimal usage conditions and stability.
Stability Testing: Tests the thermal stability, light stability, and other properties of the dye to ensure it remains effective during cell staining procedures.
Cytotoxicity Assessment: Assesses the toxicity of the dye in cell staining applications using methods like MTT to ensure biocompatibility.
Adsorption and Binding Capacity Testing: Measures the dye's ability to bind to cell surfaces or other biomolecules, ensuring specificity and efficiency in staining.

Advanced Analytical Platform

High-Performance Liquid Chromatography (HPLC)
Mass Spectrometry (MS)
Ultraviolet-Visible Spectrophotometry (UV-Vis)
Gas Chromatography (GC)
Fluorescence Spectral Analysis
Nuclear Magnetic Resonance (NMR)
Mass Spectrometry (MS)
Dynamic Light Scattering (DLS)

Which Cell Staining Application Do You Need Support For?

The cell staining reagents offered by BOC Sciences are suitable for a variety of cell analysis applications, including cell proliferation, apoptosis, division, and the localization of different molecular markers within cells. With highly sensitive, specific, and compatible staining reagents, researchers can achieve precise cell observation, advancing research in cell biology and related fields. Additionally, BOC Sciences' staining products support personalized customization to meet the specific needs of different industries and research topics, providing strong technical support for scientific research.

Cell Morphology and Structure Analysis Fluorescent dyes are widely used for cell morphology and structure analysis. By labeling specific substructures of cells, such as the nucleus, cell membrane, mitochondria, etc., researchers can observe morphological changes using fluorescence microscopy. The selectivity of the dyes allows for labeling specific cellular regions, providing an ideal tool for detailed studies of cell structure. This application is commonly used in cell proliferation, morphological changes, and drug-induced cellular response studies.
Protein Localization and Quantification Fluorescent dyes can bind to specific antibodies to label target proteins within cells. By using antibody-fluorescent dye conjugates, researchers can precisely observe the cellular localization of proteins under the microscope and study their distribution in different cell types or conditions. Additionally, by combining the intensity of the fluorescent signal, quantitative analysis of proteins can be conducted to reveal their roles in biological processes.
Live Cell Imaging Fluorescent dyes are suitable not only for staining fixed cells but also for live cell labeling, allowing real-time monitoring of intracellular dynamic changes. For example, they can be used to observe biological processes such as cell division, intracellular material transport, and ion flux. Using fluorescence imaging technology, researchers can non-invasively observe the function and changes of live cells, significantly advancing research in cell biology, drug screening, and toxicity evaluation.
Multiplexing and Co-localization Experiments Multiplexing experiments use fluorescent dyes with different wavelengths to label multiple components of cells simultaneously for co-localization analysis of various molecules within the cell. This approach allows researchers to reveal interactions, collaboration, and spatial distribution of different molecules inside the cell. For example, studying the interaction between the cell membrane and the endoplasmic reticulum or the cooperation of two different signaling pathways within the same cell.
Apoptosis Detection Fluorescent dyes can be used to detect hallmark changes during the apoptosis process, such as cell membrane damage, DNA fragmentation, or activation of specific apoptotic proteins. By labeling these apoptotic features, researchers can quantitatively analyze the extent and distribution of apoptosis. This technology is widely applied in cancer, immunology research, and drug development to assess the ability of drugs to induce cell apoptosis.
Flow Cytometry Fluorescent dyes play an important role in flow cytometry, where they can label cell surface molecules, DNA content, or other intracellular components. This enables researchers to quickly and accurately analyze the characteristics of a large number of cells, such as surface markers, cell cycle, or cell division or death status. Combined with flow cytometry, fluorescent dyes enable efficient screening and classification of complex cell populations and are widely used in immunology, cancer research, and drug screening.

FAQs About Cell Staining

What is cell staining and what is it used for? Cell staining refers to the process of staining cells using dyes or fluorescent markers to make their structures, morphology, or specific molecules more visible under a microscope. Staining techniques are widely used in cytology, molecular biology, and pathology to help researchers observe cell morphology, analyze intracellular structures, or detect specific cell markers, proteins, or gene expression.
What is the cell cycle staining method? The cell cycle staining method involves using specific dyes to label cells to analyze their distribution across different cycle stages. Commonly used dyes, such as Hoechst and DAPI, stain DNA, and flow cytometry or fluorescence microscopy can be used to observe the cell cycle process, helping to study cell division, proliferation, and regulation mechanisms.
What dye is used to stain live cells? Dyes used for staining live cells typically have low toxicity and can penetrate cell membranes. Common dyes include propidium iodide (PI), Calcein-AM, and Hoechst 33342. These dyes are used for observing live cell morphology, distinguishing live and dead cells, or visualizing intracellular components, typically under fluorescence microscopy.

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Cell Staining

What is Cell Staining?

Transform Your Experiments with Superior Fluorescent Dye Technology

Fluorescent Dye Solutions to Support Various Cell Staining Techniques

Organelle Staining

Live Cell Tracking

Ion and Redox State Detection Probes

Exploring the Most Popular List of Cell Staining Dyes

Advanced Quality Control Techniques Ensure Accuracy of Results

Advanced Analytical Platform

Which Cell Staining Application Do You Need Support For?

Cell Morphology and Structure Analysis

Protein Localization and Quantification

Live Cell Imaging

Multiplexing and Co-localization Experiments

Apoptosis Detection

Flow Cytometry

FAQs About Cell Staining

What is cell staining and what is it used for?

What is the cell cycle staining method?

What dye is used to stain live cells?