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FITC isomer I | 3326-32-7
| Catalog Number | F04-0012 |
| Category | Fluorescein FAM |
| Molecular Formula | C21H11NO5S |
| Molecular Weight | 389.38 |
| Catalog Number | Size | Price | Quantity |
|---|---|---|---|
| F04-0012 | 25 g | $1999 |
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Product Introduction
FITC isomer I is an amine-reactive fluorescent probe for labeling of proteins as well as microsequencing of proteins and peptides.
Chemical Information |
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|---|---|
| Synonyms | Fluorescein 5-Isothiocyanate (isomer I);FITC, Fluorescein isothiocyanate;Fluorescein-5-isothiocyanate; Fluorescein isothiocyanate isomer I |
| Purity | >98% |
| Canonical SMILES | C1=CC2=C(C=C1N=C=S)C(=O)OC23C4=C(C=C(C=C4)O)OC5=C3C=CC(=C5)O |
| InChI | InChI=1S/C21H11NO5S/c23-12-2-5-16-18(8-12)26-19-9-13(24)3-6-17(19)21(16)15-4-1-11(22-10-28)7-14(15)20(25)27-21/h1-9,23-24H |
| InChI Key | MHMNJMPURVTYEJ-UHFFFAOYSA-N |
| Density | 1.54 g/cm3 |
| Appearance | Solid Powder |
| Boiling Point | 708.6°C at 760 mmHg |
| Melting Point | 360°C |
- Product Specification
- Application
| Maximum Absorption Wavelength | 499 nm |
| Excitation | 490-500 nm |
| Emission | 510-525 nm |
| Condition To Avoid | Light Sensitive,Moisture Sensitive,Heat Sensitive |
| Storage | -20 °C |
| Store Under Inert Gas | Store under inert gas |
FITC isomer I, a versatile fluorescent dye, finds widespread use in a multitude of biological and biomedical applications. Here are four key applications of FITC isomer I:
Flow Cytometry: Embracing FITC isomer I, researchers harness its fluorescence in flow cytometry to target and detect specific cell subsets. By combining FITC with antibodies, they can precisely quantify the expression of surface and intracellular markers within heterogeneous cell populations. This detailed analysis unveils the intricate profiles of immune cells, cancer cells, and other cellular variants, shedding light on their unique characteristics.
Fluorescence Microscopy: Delving into fluorescence microscopy, FITC isomer I emerges as a pivotal tool for visualizing cellular components and tracking protein distributions. When linked to antibodies or proteins, FITC illuminates the inner workings of cells, allowing researchers to monitor the movements and arrangements of cellular structures under the lens of a fluorescent microscope. This application offers critical insights into cellular dynamics in both fixed and live cell environments.
Immunoassays: In immunoassays like ELISA and Western blotting, FITC isomer I plays a vital role in the detection of specific antigens or antibodies. By conjugating FITC to detection antibodies, researchers can quantitatively measure the presence of target molecules through fluorescence signals. This technique enhances the sensitivity and accuracy of diagnostic and research assays, facilitating the precise identification of key biomarkers in biological samples.
Cell Sorting: Empowered by FITC isomer I, scientists leverage its fluorescent properties for sorting live cells based on distinct markers using fluorescence-activated cell sorting (FACS). This cutting-edge technique enables the isolation and purification of specific cell populations for subsequent genomic analyses or cell culture experiments. FITC-labeled cells can be sorted with precision based on their fluorescent intensities, paving the way for advanced studies in cell biology and molecular research.
Applications of Fluorescent Probes & Dyes
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