3'-(6-FAM) CPG

3'-(6-FAM) CPG is a solid-phase controlled pore glass (CPG) support functionalized with a 6-FAM fluorophore at the 3' end, enabling direct incorporation of a fluorescent label during oligonucleotide synthesis. Researchers use this reagent to generate fluorescently tagged DNA or RNA strands for nucleic acid analysis workflows where the dye is positioned at a defined terminus for consistent readout in fluorescence-based instrumentation.

1. Fluorescent Oligonucleotide Probes

3'-(6-FAM) CPG supports the construction of terminally labeled oligonucleotide probes used in hybridization and nucleic-acid binding studies. Common workflows include designing fluorescently tagged DNA probes for monitoring target-dependent hybridization, tracking probe incorporation into assay formats, and producing sequence-defined fluorescence standards for method development in molecular biology laboratories.

2. Fluorescence-Based Hybridization Assays

3'-(6-FAM) CPG is frequently used to prepare FAM-labeled oligonucleotides for fluorescence hybridization assays, where signal readout is driven by probe-target association and the resulting fluorescence behavior in solution or on membranes. This format is particularly convenient for researchers developing nucleic acid workflows for endpoint fluorescence measurements, including probe optimization studies and assay reagent screening in molecular diagnostics R&D settings.

3. Sequencing And Fragment Analysis Standards

3'-(6-FAM) CPG enables the generation of 6-FAM-labeled oligonucleotide fragments and calibration materials with a defined 3' fluorophore placement. Molecular biology groups use these labeled strands to support fluorescence-based sizing, fragment tracking, and method validation for nucleic acid analysis pipelines that rely on terminal dye positioning for reproducible signal generation across runs.