Fluorescein-DBCO

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Fluorescein-DBCO

Fluorescein-DBCO | 2054339-00-1

Catalog Number F04-0008
Category Fluorescein FAM
Molecular Formula C39H27N3O6S
Molecular Weight 665.71
Catalog Number Size Price Quantity
F04-0008 -- $--

Chemical Information

Synonyms DBCO-FITC(5-isomer)
Purity >98.0%
Shelf Life 0-4°C for short term (days to weeks), or -20°C for long term (months).
Canonical SMILES C1C2=CC=CC=C2C#CC3=CC=CC=C3N1C(=O)CCNC(=S)NC4=CC5=C(C=C4)C6(C7=C(C=C(C=C7)O)OC8=C6C=CC(=C8)O)OC5=O
InChI InChI=1S/C39H27N3O6S/c43-27-12-15-31-34(20-27)47-35-21-28(44)13-16-32(35)39(31)30-14-11-26(19-29(30)37(46)48-39)41-38(49)40-18-17-36(45)42-22-25-7-2-1-5-23(25)9-10-24-6-3-4-8-33(24)42/h1-8,11-16,19-21,43-44H,17-18,22H2,(H2,40,41,49)
InChI Key FGEDVMJBPDYXLU-UHFFFAOYSA-N
Solubility 10 mm in DMSO
Appearance Solid
  • Product Specification
  • Application
Excitation 494
Emission 517
Storage Store at -5°C,keep in dry and avoid sunlight.

Fluorescein-DBCO, a compound with fluorescent labeling, plays a pivotal role in various biochemical applications. Explore four key applications of Fluorescein-DBCO with enhanced perplexity and burstiness:

Bioorthogonal Chemistry: Leveraging Fluorescein-DBCO in bioorthogonal chemistry allows for the precise labeling of biomolecules without interference. When coupled with azide-containing molecules, it facilitates the visualization and tracking of these biomolecules within living cells. This application is indispensable for real-time examination of cellular processes, shedding light on intricate biological interactions.

Protein Labeling: By enabling the fluorescent labeling of proteins, Fluorescein-DBCO simplifies the detection and analysis of protein interactions and functions. This capability proves particularly beneficial in fluorescence microscopy and flow cytometry, where labeled proteins can be monitored within cells or scrutinized for binding events and functional assessments. Researchers can delve deeply into protein dynamics and cellular localization, unraveling the complexities of cellular behavior.

DNA and RNA Labeling: Fluorescein-DBCO serves as a valuable tool for labeling nucleic acids, aiding in the investigation of gene expression and nucleic acid-protein interactions. This application allows for the visualization of DNA and RNA within cells, contributing to the understanding of transcription, replication, and translation mechanisms. The fluorescently labeled nucleic acids offer critical insights into genome organization and regulation, enhancing our understanding of genetic processes.

Drug Delivery Research: In the realm of drug delivery studies, Fluorescein-DBCO can be conjugated to therapeutic molecules, enabling the monitoring of their distribution and localization within biological systems. This capability empowers researchers to track the delivery and release of drugs at the cellular level, ensuring targeted delivery to intended sites. Such applications are essential for optimizing drug delivery systems and enhancing therapeutic efficacy, advancing the landscape of drug development and administration.

Computed Properties

XLogP3 5.6
Hydrogen Bond Donor Count 4
Hydrogen Bond Acceptor Count 7
Rotatable Bond Count 4
Exact Mass 665.16205676 g/mol
Monoisotopic Mass 665.16205676 g/mol
Topological Polar Surface Area 152Ų
Heavy Atom Count 49
Formal Charge 0
Complexity 1320
Isotope Atom Count 0
Defined Atom Stereocenter Count 0
Undefined Atom Stereocenter Count 0
Defined Bond Stereocenter Count 0
Undefined Bond Stereocenter Count 0
Covalently-Bonded Unit Count 1
Compound Is Canonicalized Yes
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