5'-(5-Fluorescein) phosphoramidite

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5'-(5-Fluorescein) phosphoramidite

5'-(5-Fluorescein) phosphoramidite | 147566-42-5

Catalog Number F04-0028
Category Fluorescein FAM
Molecular Formula C46H58N3O10P
Molecular Weight 843.94
Catalog Number Size Price Quantity
F04-0028 -- $--

Product Introduction

5'-(5-Fluorescein) phosphoramidite is a reagent used in the synthesis of fluorescently labeled oligonucleotides. FAM (Fluorescein Amidite) is a fluorescent dye, and the 5-isomer refers to the specific structural form of FAM that is commonly used for labeling. It is a key reagent for researchers needing to create fluorescently labeled DNA or RNA for various diagnostic, research, and analytical applications.

Chemical Information

Synonyms 5-FAM phosphoramidite; 5-Fluorescein Phosphoramidite; 5'(5)-FAM; 5'-5-Fluorescein phosphoramidite; FAM Phosphoramidite, 5-Isomer; Propanoic acid, 2,2-dimethyl-, 5-[10-[bis(1-methylethyl)amino]-13-cyano-1-oxo-9,11-dioxa-2-aza-10-phosphatridec-1-yl]-3-oxospiro[isobenzofuran-1(3H),9'-[9H]xanthene]-3',6'-diyl ester; Fluorescein Phosphoramidite
Purity >95%
IUPAC Name [5-[6-[2-cyanoethoxy-[di(propan-2-yl)amino]phosphanyl]oxyhexylcarbamoyl]-6'-(2,2-dimethylpropanoyloxy)-3-oxospiro[2-benzofuran-1,9'-xanthene]-3'-yl] 2,2-dimethylpropanoate
Canonical SMILES CC(C)N(C(C)C)P(OCCCCCCNC(=O)C1=CC2=C(C=C1)C3(C4=C(C=C(C=C4)OC(=O)C(C)(C)C)OC5=C3C=CC(=C5)OC(=O)C(C)(C)C)OC2=O)OCCC#N
InChI InChI=1S/C46H58N3O10P/c1-29(2)49(30(3)4)60(55-25-15-22-47)54-24-14-12-11-13-23-48-40(50)31-16-19-35-34(26-31)41(51)59-46(35)36-20-17-32(56-42(52)44(5,6)7)27-38(36)58-39-28-33(18-21-37(39)46)57-43(53)45(8,9)10/h16-21,26-30H,11-15,23-25H2,1-10H3,(H,48,50)
InChI Key XUQUNBOKLNVMMK-UHFFFAOYSA-N
Solubility Soluble in Acetonitrile, DCM
Appearance White to off-white solid
Boiling Point 844.7±65.0 °C at 760 mmHg
  • Product Specification
  • Application
Storage Store at -20 °C
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