5'-(5-Fluorescein) phosphoramidite | CAS 147566-42-5

Name: 5'-(5-Fluorescein) phosphoramidite
Brand: BOC Sciences
Rating: 5 (1 reviews)

Catalog Number	F04-0028
Category	Fluorescein FAM
Molecular Formula	C46H58N3O10P
Molecular Weight	843.94

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Product Introduction

5'-(5-Fluorescein) phosphoramidite is a fluorophore-bearing reagent utilized in the synthesis of fluorescently labeled oligonucleotides. This compound features a fluorescein chromophore known for its distinct excitation and emission properties, making it suitable for incorporation into DNA and RNA sequences for fluorescence-based detection and imaging applications. The phosphoramidite functionality enables efficient conjugation to nucleic acids, facilitating its use in fluorescence resonance energy transfer (FRET) studies and other bioanalytical assays.

Chemical Information
Product Specification

Chemical Information

Synonyms	5-FAM phosphoramidite; 5-Fluorescein Phosphoramidite; 5'(5)-FAM; 5'-5-Fluorescein phosphoramidite; FAM Phosphoramidite, 5-Isomer; Propanoic acid, 2,2-dimethyl-, 5-[10-[bis(1-methylethyl)amino]-13-cyano-1-oxo-9,11-dioxa-2-aza-10-phosphatridec-1-yl]-3-oxospiro[isobenzofuran-1(3H),9'-[9H]xanthene]-3',6'-diyl ester; Fluorescein Phosphoramidite
Purity	>95%
IUPAC Name	[5-[6-[2-cyanoethoxy-[di(propan-2-yl)amino]phosphanyl]oxyhexylcarbamoyl]-6'-(2,2-dimethylpropanoyloxy)-3-oxospiro[2-benzofuran-1,9'-xanthene]-3'-yl] 2,2-dimethylpropanoate
SMILES	CC(C)N(C(C)C)P(OCCCCCCNC(=O)C1=CC2=C(C=C1)C3(C4=C(C=C(C=C4)OC(=O)C(C)(C)C)OC5=C3C=CC(=C5)OC(=O)C(C)(C)C)OC2=O)OCCC#N
InChI	InChI=1S/C46H58N3O10P/c1-29(2)49(30(3)4)60(55-25-15-22-47)54-24-14-12-11-13-23-48-40(50)31-16-19-35-34(26-31)41(51)59-46(35)36-20-17-32(56-42(52)44(5,6)7)27-38(36)58-39-28-33(18-21-37(39)46)57-43(53)45(8,9)10/h16-21,26-30H,11-15,23-25H2,1-10H3,(H,48,50)
InChIKey	XUQUNBOKLNVMMK-UHFFFAOYSA-N
Solubility	Soluble in Acetonitrile, DCM
Appearance	White to off-white solid
Boiling Point	844.7±65.0 °C at 760 mmHg