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5-Carboxyfluorescein | CAS 76823-03-5
| Catalog Number | F04-0024 |
| Category | Fluorescein FAM |
| Molecular Formula | C21H12O7 |
| Molecular Weight | 376.32 |
* Please be kindly noted products are not for therapeutic use. We do not sell to patients.
Product Introduction
5-Carboxyfluorescein is a derivative of fluorescein that can be used to label biomolecules through interacting with primary amines.
Chemical Information
Product Specification
Application
Computed Properties
Literatures
Patents
| Synonyms | 4-carboxyfluorescein; 5-FAM; 3',6'-Dihydroxy-3-oxo-3H-spiro[isobenzofuran-1,9'-xanthene]-5-carboxylic acid |
| Purity | ≥ 95 % |
| IUPAC Name | 3',6'-dihydroxy-3-oxospiro[2-benzofuran-1,9'-xanthene]-5-carboxylic acid |
| Canonical SMILES | C1=CC2=C(C=C1C(=O)O)C(=O)OC23C4=C(C=C(C=C4)O)OC5=C3C=CC(=C5)O |
| InChI | InChI=1S/C21H12O7/c22-11-2-5-15-17(8-11)27-18-9-12(23)3-6-16(18)21(15)14-4-1-10(19(24)25)7-13(14)20(26)28-21/h1-9,22-23H,(H,24,25) |
| InChIKey | NJYVEMPWNAYQQN-UHFFFAOYSA-N |
| Solubility | Soluble in DMF; Slightly soluble in DMSO, Methanol |
| Density | 1.73±0.1 g/cm3 (Predicted) |
| Appearance | Yellow solid |
| Boiling Point | 725.2±60.0 °C (Predicted) |
| MDL Number | MFCD00036874 |
| LogP | 3.36400 |
| Excitation | 492 nm |
| Emission | 518 nm |
| Condition To Avoid | Light Sensitive |
| Storage | -20 °C |
| Signal | Warning |
| GHSHazardStatements | H315 (20%): Causes skin irritation [Warning Skin corrosion/irritation] H319 (100%): Causes serious eye irritation [Warning Serious eye damage/eye irritation] |
| Precautionary Statement Codes | P264, P264+P265, P280, P302+P352, P305+P351+P338, P321, P332+P317, P337+P317, and P362+P364 (The corresponding statement to each P-code can be found at the GHS Classification page.) |
5-Carboxyfluorescein is a fluorescent dye used extensively in biological and chemical research. Here are some key applications of 5-Carboxyfluorescein:
Fluorescence Microscopy: 5-Carboxyfluorescein is widely used in fluorescence microscopy to label and image biological molecules, cells, and tissues. Its bright green fluorescence allows for the visualization of cellular structures and protein localization with high specificity. This enables researchers to study dynamic processes in live cells without significant phototoxicity.
Flow Cytometry: In flow cytometry, 5-Carboxyfluorescein is used to distinguish different cell populations based on fluorescence intensity. Cells stained with this dye can be quantitatively analyzed for various parameters such as size, granularity, and surface protein expression. This application is critical in immunology, cancer research, and cell sorting techniques.
Molecular Probes: 5-Carboxyfluorescein serves as a molecular probe in assays to study enzyme activity, receptor-ligand interactions, and other biochemical processes. By conjugating the dye to specific molecules, researchers can monitor real-time changes in fluorescence that indicate molecular interactions. This is essential for high-throughput screening in drug discovery and functional genomics studies.
Quantitative PCR (qPCR): 5-Carboxyfluorescein is used as a reporter dye in quantitative PCR to detect and quantify nucleic acids. It fluoresces proportionally to the amount of PCR product, allowing for precise measurement of gene expression levels. This application is pivotal in diagnostic applications, gene expression profiling, and genetic research.
| XLogP3 | 2.9 |
| Hydrogen Bond Donor Count | 3 |
| Hydrogen Bond Acceptor Count | 7 |
| Rotatable Bond Count | 1 |
| Exact Mass | 376.05830272 g/mol |
| Monoisotopic Mass | 376.05830272 g/mol |
| Topological Polar Surface Area | 113Ų |
| Heavy Atom Count | 28 |
| Formal Charge | 0 |
| Complexity | 637 |
| Isotope Atom Count | 0 |
| Defined Atom Stereocenter Count | 0 |
| Undefined Atom Stereocenter Count | 0 |
| Defined Bond Stereocenter Count | 0 |
| Undefined Bond Stereocenter Count | 0 |
| Covalently-Bonded Unit Count | 1 |
| Compound Is Canonicalized | Yes |
| PMID | Publication Date | Title | Journal |
|---|---|---|---|
| 22387888 | 2012-05-30 | Suppression of efflux transporters in the intestines of endotoxin-treated rats | International journal of pharmaceutics |
| 22545861 | 2012-05-16 | In vitro selection of functional lantipeptides | Journal of the American Chemical Society |
| 22276994 | 2012-04-20 | Cyclic peptide inhibitors of HIV-1 capsid-human lysyl-tRNA synthetase interaction | ACS chemical biology |
| 22272228 | 2012-01-01 | A reliable method for the selection of exploitable melanoma archival paraffin embedded tissues for transcript biomarker profiling | PloS one |
| 22295088 | 2012-01-01 | The kinetics of early T and B cell immune recovery after bone marrow transplantation in RAG-2-deficient SCID patients | PloS one |
| Publication Number | Title | Priority Date |
|---|---|---|
| CN-115192728-A | A kind of prodrug micelle and its preparation method and use | 2022-07-20 |
| CN-114805536-A | A kind of human platelet factor 4 protein mutant and its application | 2022-06-24 |
| CN-114805536-B | A kind of human platelet factor 4 protein mutant and its application | 2022-06-24 |
| CN-114807315-A | Method and application of Ψ-hybridization and Cas12a-assisted signal amplification to detect microRNA | 2022-06-21 |
| CN-115028716-A | A kind of monoclonal antibody and its application | 2022-06-10 |
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