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6-AminoFluorescein | 51649-83-3
| Catalog Number | F04-0023 |
| Category | Fluorescein FAM |
| Molecular Formula | C20H13NO5 |
| Molecular Weight | 347.33 |
| Catalog Number | Size | Price | Quantity |
|---|---|---|---|
| F04-0023 | -- | $-- |
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Product Introduction
6-AminoFluorescein is an indispensable fluorescent dye in the realm of biomedical research. Widely acknowledged for its paramount role in the genesis of diagnostic assays and biomolecule labeling, particularly nucleic acids and proteins, it unravels a new dimension in the detection and visualization of target molecules. Its acute sensitivity and harmonious compatibility render it a powerful tool in disease-related investigations pertaining to cancer diagnostics, pathogen identification, and drug discovery endeavors.
Chemical Information |
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|---|---|
| Synonyms | 6-Aminofluorescein (isomer II);6-AMINOFLUORESCEIN; 6-AMINO-3',6'-DIHYDROXYSPIRO[ISOBENZOFURAN-1(3H),9'-(9H)XANTHEN]-3-ONE; 6-amino-3',6'-dihydroxyspiro[isobenzofuran-1(3h),9'-[9h]xanthene]-3-one; AMINOFLUORESCEIN ISOMER II; 5-AMINOFLUORESCEIN (ISOMER II); FLUORESCEINAMINE, ISOMER 2 |
| Purity | >95.0%(T) |
| IUPAC Name | 5-amino-3',6'-dihydroxyspiro[2-benzofuran-3,9'-xanthene]-1-one |
| Canonical SMILES | C1=CC2=C(C=C1N)C3(C4=C(C=C(C=C4)O)OC5=C3C=CC(=C5)O)OC2=O |
| InChI | InChI=1S/C20H13NO5/c21-10-1-4-13-16(7-10)20(26-19(13)24)14-5-2-11(22)8-17(14)25-18-9-12(23)3-6-15(18)20/h1-9,22-23H,21H2 |
| InChI Key | YOAWSYSKQHLFPM-UHFFFAOYSA-N |
| Density | 1.64 g/cm3 |
| Appearance | Yellow Solid |
| Boiling Point | 706.7°C at 760 mmHg |
| Melting Point | 285°C (dec.) |
| MDL Number | MFCD00005051 |
- Product Specification
- Application
| Condition To Avoid | Light Sensitive |
| Storage | 2-8°C |
| Signal | Warning |
| GHS Hazard Statements | H315 (100%): Causes skin irritation [Warning Skin corrosion/irritation] H319 (100%): Causes serious eye irritation [Warning Serious eye damage/eye irritation] H335 (100%): May cause respiratory irritation [Warning Specific target organ toxicity, single exposure; Respiratory tract irritation] |
| Precautionary Statement Codes | P261, P264, P264+P265, P271, P280, P302+P352, P304+P340, P305+P351+P338, P319, P321, P332+P317, P337+P317, P362+P364, P403+P233, P405, and P501 (The corresponding statement to each P-code can be found at the GHS Classification page.) |
6-AminoFluorescein, a versatile fluorescent dye, with myriad applications in scientific and medical domains. Here are four key applications of 6-AminoFluorescein:
Fluorescence Microscopy: Widely employed in microscopy, 6-AminoFluorescein serves as a fluorescent probe for visualizing and tracking cellular components. By tagging antibodies or other biomolecules, it facilitates the detection and localization of specific proteins or structures within cells, aiding in the exploration of cell biology, molecular interactions, and spatial organization in living tissues.
Flow Cytometry: A vital tool in the study of cell populations, 6-AminoFluorescein is utilized in flow cytometry as a fluorescent marker for labeling specific cell surface proteins or intracellular molecules. This enables researchers to quantify and analyze various parameters of individual cells, such as cell size, granularity, and protein expression levels, crucial for immunophenotyping, cell sorting, and comprehension of cellular diversity.
DNA Sequencing: In automated DNA sequencing techniques, 6-AminoFluorescein plays a pivotal role as a fluorescent label. By binding it to nucleotides or DNA fragments, researchers can detect sequence information via fluorescence signals, fundamental for genomics research, clinical diagnostics, and the advancement of personalized medicine.
Live Cell Imaging: Researchers harness 6-AminoFluorescein for live-cell imaging to monitor dynamic processes in real-time. By integrating it into live cells, they can observe phenomena like protein interactions, cellular trafficking, and changes in cell morphology. This practice enhances our understanding of cellular functions and supports the development of innovative therapeutic approaches.
Computed Properties | |
|---|---|
| XLogP3 | 2.7 |
| Hydrogen Bond Donor Count | 3 |
| Hydrogen Bond Acceptor Count | 6 |
| Rotatable Bond Count | 0 |
| Exact Mass | 347.07937252 g/mol |
| Monoisotopic Mass | 347.07937252 g/mol |
| Topological Polar Surface Area | 102Ų |
| Heavy Atom Count | 26 |
| Formal Charge | 0 |
| Complexity | 557 |
| Isotope Atom Count | 0 |
| Defined Atom Stereocenter Count | 0 |
| Undefined Atom Stereocenter Count | 0 |
| Defined Bond Stereocenter Count | 0 |
| Undefined Bond Stereocenter Count | 0 |
| Covalently-Bonded Unit Count | 1 |
| Compound Is Canonicalized | Yes |
Literatures
| PMID | Publication Date | Title | Journal |
|---|---|---|---|
| 17683530 | 2007-08-02 | Self assembly of amphiphilic C60 fullerene derivatives into nanoscale supramolecular structures | Journal of nanobiotechnology |
| 1964628 | 1990-12-01 | Potentiation of the antiviral activity of poly r(A-U) by xanthene dyes | Cell biology international reports |
Patents
| Publication Number | Title | Priority Date |
|---|---|---|
| CN-115040535-B | Application of Sulfated Hyaluronic Acid in Preparation of Eye Drops for Prevention of Corneal Fibrosis and Corneal Scar | 2022-08-15 |
| WO-2022207813-A1 | Mitochondrial ph probes and uses thereof | 2021-03-31 |
| CN-113117172-A | Blood simulation medium and preparation method and application thereof | 2021-03-29 |
| CN-114755424-A | Supports, kits and methods for fluorescence polarization immunoassays | 2021-01-08 |
| US-2022221465-A1 | Support for fluorescence polarization immunoassay, fluorescence polarization immunoassay kit and fluorescence polarization immunoassay | 2021-01-08 |
Applications of Fluorescent Probes & Dyes
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