Tissue Engineering
BOC Sciences focuses on providing high-performance fluorescent dyes and probe solutions for the field of tissue engineering, establishing a one-stop service system from molecular design, custom synthesis, and biological labeling to optical validation. Relying on a strong platform for organic synthesis and labeling technologies, we are able to flexibly meet personalized demands involving different wavelengths, functional groups, and targeting objects, supporting researchers in the precise visualization of cellular functions, tissue construction, and material behavior. Our service process is standardized and efficient, with rapid response and multi-stage delivery from milligram-level trials to hundred-gram-scale production. In addition, we are equipped with multiple advanced analytical instruments to ensure excellent purity, photostability, and labeling efficiency for each product batch.
BOC Sciences – Your Trusted Partner in Fluorescent Solutions
High Purity and Batch-to-Batch Consistency
BOC Sciences strictly controls the purity of fluorescent dyes (typically ≥ 95%), ensuring stable signals, low background, and clear imaging between batches.Broad Wavelength Coverage and Near-Infrared Options
Our products span UV to near-infrared wavelengths (350–850 nm), suitable for a wide range of microscopy systems and deep-tissue visualization needs.Custom Synthesis and Covalent Conjugation Services
We offer custom services for fluorescent labeling of proteins, peptides, antibodies, and polymers for specialized applications, and support the simultaneous use of multicolor dyes.High-Throughput Screening and In Vivo Imaging Support
Our fluorescent probes are applicable to tissue microarrays, 3D spheroids, and animal models, compatible with confocal, multiphoton, and IVIS imaging systems.
Core Fluorescent Solutions Supporting Tissue Engineering
In the complex and precise research systems of tissue engineering, selecting suitable fluorescent dyes and probes is crucial to ensure experimental success and data reliability. Based on a robust chemical synthesis platform and molecular labeling technologies, BOC Sciences has developed a multi-dimensional product system covering cell labeling, tissue-specific imaging, environment-responsive probes, and scaffold material visualization, fully addressing the diverse needs from basic research to clinical translation.
Cell Labeling Dyes
BOC Sciences provides a variety of fluorescent dyes for labeling cell membranes, cytoplasm, and nuclei:
- Membrane labeling dyes: such as DiI, DiO, PKH26, used for long-term tracking of cell migration and fusion.
- Nuclear dyes: such as DAPI and Hoechst series, commonly used for nuclear visualization in confocal imaging.
- Viability dyes: such as Calcein-AM (live cell marker), PI or EthD-1 (dead cell markers).
Tissue-Specific Probes and Antibody Fluorescent Labeling
BOC Sciences offers high-purity fluorescent-labeled antibodies and targeted probes for the detection of different cell differentiation pathways and tissue types:
- Bone tissue engineering: antibodies labeled with Osteocalcin, ALP, Runx2.
- Cardiac tissue engineering: probes for cTnT, α-actinin.
- Neural tissue engineering: dyes labeling GFAP, β-III Tubulin, Nestin.
- Angiogenesis monitoring: markers including CD31, VEGF, vWF.
Environment-Responsive Fluorescent Probes
Microenvironment parameters (e.g., pH, ROS, Ca²⁺, O₂) significantly affect tissue construction quality. BOC Sciences provides highly sensitive responsive probes, including:
- pH probes: BCECF-AM, LysoSensor.
- Calcium ion probes: Fluo-4 AM, Fura-2.
- Reactive oxygen species (ROS) probes: DCFH-DA, MitoSOX.
- Oxygen probes: customizable fluorescence-quenching-based O₂ detection probes.
Scaffold Material Labeling Dyes
To assess the behavior of biodegradable materials in tissue construction, BOC Sciences offers fluorescent labeling solutions for polymers and collagen scaffolds, including:
- Fluorescently labeled scaffold dyes: applicable to biomaterials such as gelatin, sodium alginate, and polylactic acid.
- Material degradation monitoring probes: fluorescence probes incorporating FRET mechanisms to track material degradation in real time.
- Biocompatibility testing dyes: used in conjunction with cell viability assays to evaluate scaffold cytotoxicity.
Fluorescence Customization Services Supporting Rapid Translation
In high-precision research and application areas like tissue engineering, different experimental systems often require unique specifications for fluorescent dye wavelength ranges, functional group types, binding mechanisms, and biocompatibility. BOC Sciences deeply understands the diverse demands in molecular labeling, imaging efficiency, and system compatibility, and has established a dedicated custom service platform to provide flexible and efficient one-stop fluorescent dye/probe solutions, facilitating the efficient translation of research outcomes to clinical or industrial applications.
Custom Dye Synthesis
ServicesWe support directional molecular design and synthesis based on specified excitation/emission wavelengths, dye core structures, or application environment needs. By adjusting functional group structures, polarity, conjugation system length, and modification sites, we can customize fluorescence intensity, photostability, water/lipid solubility to suit diverse applications such as multi-channel fluorescence imaging, deep-tissue detection, or near-infrared imaging.
Biomolecule Labeling
ServicesBOC Sciences provides fluorescent labeling services for antibodies, peptides, and proteins, ensuring efficient and stable conjugation of dyes while preserving biological activity and targeting capability. We employ a variety of labeling strategies (e.g., NHS esters, maleimides, click chemistry), and offer labeling purification and degree-of-labeling analysis services, compatible with various immunofluorescence, tracking, and biodistribution studies.
Covalent Modification Services for
Nanoparticles and PolymersFor commonly used nanoparticles, biodegradable polymers, or scaffold materials in tissue engineering, we provide fluorescent labeling modification services to enable real-time monitoring of material distribution, cell-material interactions, and in vivo degradation. We specialize in constructing surface functionalization strategies to achieve stable and uniform covalent bonding between probes and materials.
Fluorophore Pair and FRET
Probe Design ServicesBOC Sciences supports clients in the screening and optimization of fluorescence resonance energy transfer (FRET) pairs, widely used in live-cell imaging, enzyme activity monitoring, and protein-protein interaction analysis. We offer comprehensive services from donor/acceptor dye selection and linker design to probe construction, ensuring energy transfer efficiency and biological system compatibility.
Standardized Service Workflow and Professional Analytical Platform
To meet the dual demands for high performance and consistency in fluorescence tools for tissue engineering, BOC Sciences has established a standardized service workflow and a multidimensional analytical platform. We provide comprehensive support from design and synthesis to delivery, equipped with advanced analytical instruments to ensure each fluorescent dye or probe exhibits excellent optical performance and structural reliability, thereby offering strong support for scientific research and industrial translation.
- Project Consultation & Requirement Confirmation: Clients can submit application details, target molecules, fluorescence specifications (e.g., wavelength, polarity, reactivity), and desired parameters via online form, email, or account manager. Our team responds within 24 hours to initiate technical engagement.
- Molecular Design & Route Evaluation: BOC Sciences' chemists offer structural optimization for dyes/probes based on tissue engineering needs, including wavelength tuning, functional group modifications, and polarity adjustments. Synthesis feasibility and delivery time are also evaluated.
- Custom Synthesis & Labeling Services: After proposal approval, custom synthesis begins, supporting milligram-to-hundred-gram scale. We also offer labeling for antibodies, peptides, nanoparticles, and polymers. Clients can choose from pure dyes, labeled conjugates, or pre-loaded formats.
- Analytical Validation & Data Delivery: Following rigorous quality control, products are delivered with comprehensive analytical reports (MS, NMR, UV-Vis, fluorescence spectra, labeling degree) to ensure accuracy and performance.
- Delivery & Technical Support: We offer global shipping and technical support to assist in initial application testing. Ongoing support is available for scale-up synthesis, optimization, or process transition.
Advanced Analytical Platform
- High Performance Liquid Chromatography (HPLC)
- Liquid Chromatography-Mass Spectrometry (LC-MS)
- Nuclear Magnetic Resonance Spectrometer (NMR)
- Ultraviolet-Visible Spectrophotometer (UV-Vis)
- Fluorometer
- Dynamic Light Scattering (DLS)
- Zeta Potential Analyzer
- Fluorescence Microscope
- Quantum Yield Measurement System
- Fourier Transform Infrared Spectrometer (FTIR)
- Thermogravimetric Analyzer (TGA)
- Fluorescence Labeling Degree Analyzer
Fluorescence Applications in Tissue Engineering
BOC Sciences deeply understands the multilayered and multidimensional fluorescence needs in tissue engineering research and translation. We offer high-performance dyes and customized probe solutions along the entire labeling-imaging-analysis chain, enabling researchers to achieve precise visualization in complex biological systems and accelerating the optimization of tissue construction and the industrialization of regenerative medicine.
Cell Tracing and Proliferation Analysis
Cells, as critical active components in tissue engineering, require real-time dynamic monitoring of their origin, distribution, migration, proliferation, and differentiation. Fluorescent dyes are widely used for cell labeling—for instance, membrane-labeling dyes (such as DiI and PKH26) are employed to trace stem cell localization and behavior in scaffolds, while nuclear dyes (such as Hoechst 33342) are used for cell cycle analysis. In addition, fluorescent probes assess cell viability, mitochondrial function, apoptosis, or necrosis, offering reliable quality control of active cells for tissue construction.
3D Scaffold and Biomaterial Imaging
Scaffold materials serve as the physical framework in tissue engineering. Their structural integrity, cell adhesion capabilities, biodegradation processes, and interactions with host tissue must be visually monitored. Fluorescent labeling of polymer materials, nanoparticles, or hydrogels enables imaging of their distribution in constructed tissues and their metabolic processes in vivo. Common dyes include FITC, Cy5, and Rhodamine. Moreover, intelligent fluorescent probes can respond to changes in pH, enzymatic activity, or oxygen concentration, allowing functional behavior detection of materials in microenvironments.
Microenvironment Monitoring and Signal Pathway Analysis
Microenvironmental factors such as extracellular matrix (ECM) components, nutrient concentrations, oxygen tension, and pH values significantly influence tissue construction outcomes. Fluorescent probes are used to dynamically detect changes in key molecules within the microenvironment—for example, ROS probes monitor oxidative stress levels, calcium ion probes (e.g., Fluo-4) reflect cellular signaling responses, and pH-sensitive dyes trace changes in local acid-base environments. These real-time data are of great value in optimizing tissue culture conditions and understanding cell behavior mechanisms.
Tissue Component Distribution and Functional Imaging
Once tissue construction is completed, researchers must verify whether the tissue structure, cellular configuration, and functional expression meet expectations. Immunofluorescence staining techniques employ labeled antibodies to recognize target proteins such as actin, collagen, and specific differentiation markers, enabling 3D imaging of tissue details. Combined with confocal or multiphoton fluorescence microscopy, high-resolution images of cell-scaffold interactions can be obtained, assessing the success and maturity of tissue construction.
In Situ Visualization and In Vivo Tracking
The ultimate goal of tissue engineering is to apply constructed tissues or organs in vivo for regeneration and repair. Therefore, long-term, non-invasive monitoring of post-transplantation behavior is essential. By labeling transplanted tissues, materials, or cells with near-infrared fluorescent probes (such as ICG and Cy7), in situ in vivo tracking can be achieved via small animal imaging systems. This approach is widely used in animal models for skin repair, bone regeneration, myocardial repair, etc., to evaluate graft survival, integration, and functional recovery.
FAQs
What types of fluorescent dyes or probes do you offer?
We provide small molecule fluorescent dyes, fluorescently labeled antibodies/peptides/proteins, fluorescent nanoparticle probes, and FRET pairs. These cover excitation/emission wavelengths from UV to near-infrared and are suitable for various imaging applications in cells, tissues, and materials.
Can dyes be customized based on my experimental design?
Absolutely. We support the customization of dyes with different wavelengths, hydrophilicity, and functional group structures. One-on-one design and validation are available based on your target molecule and labeling strategy.
What types of molecules can be labeled with fluorescent dyes?
We offer covalent and non-covalent fluorescent labeling for proteins, peptides, antibodies, oligonucleotides, polymers, nanoparticles, and more, meeting the application needs of various biomaterials and scaffold systems.
Do you provide labeling services for dyes?
Yes. We not only supply dyes but also offer professional labeling services, including conjugation, purification, and quality control for dye-antibody, peptide, nanoparticle, or polymer complexes.
Can I choose FRET dye combinations?
Yes. We can recommend the most suitable donor/acceptor combinations based on your experimental system to optimize FRET efficiency and support dynamic signal detection and metabolic process monitoring.
Do you support small-scale trials or sample orders?
Yes. We offer small-scale synthesis samples for solution validation and flexible deliveries ranging from milligrams to hundreds of grams to meet the needs of both research and pilot-scale production.
Are your products quality-controlled and analytically verified?
All products undergo multiple testing procedures including HPLC, LC-MS, NMR, UV-Vis, and fluorescence spectra analysis to ensure purity, structure, and optical performance. Complete analytical reports are provided.
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