Gel Electrophoresis
As a top provider in the life sciences and biotechnology markets, BOC Sciences delivers premium dyes and electrophoresis reagents, which serve research institutions as well as pharmaceutical companies and diagnostic labs. Our product line includes complete gel electrophoresis dye systems that enable electrophoretic staining of DNA, RNA, and proteins to deliver accurate and replicable experimental results. We also provide conventional dyes together with customizable solutions to fulfill various research needs. Through advanced production methods alongside strong quality control measures and superior technical assistance, we deliver dependable electrophoresis staining options to researchers worldwide, enabling efficient molecular biology investigations.
What is Gel Electrophoresis?
Gel electrophoresis is an experimental technique used to separate biomolecules under the influence of an electric field, widely used for the analysis of DNA, RNA, and proteins. By loading the sample into agarose or polyacrylamide gel and applying an electric field, molecules migrate based on characteristics such as size and charge, achieving separation. Gel electrophoresis staining refers to the use of specific dyes to visualize nucleic acids or proteins after or during the electrophoresis process, allowing analysis of the band position, concentration, and integrity. This technique is widely applied in molecular biology, genetics, proteomics, medical diagnostics, and drug development. Common staining methods include fluorescent dyes (e.g., SYBR Green, SYPRO Ruby), colorimetric dyes (e.g., Coomassie Brilliant Blue), and radioactive labeling to meet different experimental needs.
Technical Advantages of BOC Sciences in Gel Electrophoresis Dyes
Diverse Product Line
We offer a variety of dyes for DNA, RNA, and protein, covering a broad range of gel electrophoresis applications.High Purity Products
Our dyes undergo strict purification processes to ensure no contamination, enhancing signal detection accuracy.Wide Applicability
Compatible with various electrophoresis systems, such as agarose gels and polyacrylamide gels.Low Background Interference
Optimized dye molecular structures reduce background noise, improving band visualization.Excellent Stability
Our dyes exhibit good stability against light, temperature, and chemical environments, making them suitable for long-term storage.Innovative R&D Capabilities
BOC Sciences has an experienced R&D team capable of developing new dyes tailored to customer needs, enhancing experimental efficiency.Environmental and Safety Focus
We are committed to developing low-toxicity, eco-friendly dye products, reducing potential hazards to researchers and the environment.Global Supply Chain
With a robust supply chain system, we can quickly respond to customer needs and ensure timely product delivery.
Explore the Popular Gel Loading Dye List
BOC Sciences offers a variety of high-performance gel electrophoresis dyes, including DNA/RNA dyes, protein dyes, and pre-stained markers, to meet the needs of various research and industrial applications. Whether for nucleic acid detection, protein separation, or quantitative analysis, BOC Sciences' products feature high sensitivity, stability, and compatibility, making them suitable for use in both agarose gel electrophoresis (AGE) and polyacrylamide gel electrophoresis (PAGE). Below are the main categories of gel electrophoresis dyes offered by BOC Sciences and their characteristics.
| Dye Name | Application | Color | Characteristics |
| Coomassie Brilliant Blue | Primarily used for protein staining after SDS-PAGE. | Deep Blue | Binds with protein amino acid residues to form stable complexes; high sensitivity for protein quantification. |
| Silver Staining | Used for high-sensitivity staining of proteins or nucleic acids post-electrophoresis, suitable for trace sample detection. | Silver or Grayish | Extremely high sensitivity, suitable for low-concentration sample detection, but the staining process is complex and requires multiple chemical reactions. |
| Xylene Cyanol FF | Primarily used for DNA electrophoresis, tracking larger DNA fragments. | Green | Migrates slowly, suitable for tracking 4-10kb DNA fragments; commonly used with bromophenol blue to track both small and large DNA molecules. |
| SYBR Green | Used for DNA electrophoresis, especially PCR product analysis, visualizing DNA samples through fluorescence. | Green Fluorescence | High sensitivity, able to detect extremely low concentrations of DNA, low toxicity, suitable for safe laboratory operations. |
| Bromophenol Blue | Primarily used for protein and nucleic acid electrophoresis, helps track sample migration. | Deep Blue | Migrates to 3000-5000 Da position, commonly used for marking sample migration progress, helps monitor electrophoresis progress without affecting sample separation. |
| Indig o Carmine | Used for nucleic acid electrophoresis, suitable for tracking medium to large DNA molecules. | Deep Blue | Migrates slowly, suitable for electrophoresis analysis of larger DNA molecules. |
| Rhodamine | Used for fluorescent gel electrophoresis, mainly for RNA or protein electrophoresis analysis. | Red (Fluorescent) | Strong fluorescence properties, suitable for fluorescence detection. |
| Sulfo-Orange G | Used for protein electrophoresis, particularly for post-staining marked proteins. | Yellow | Used for post-protein analysis, suitable for protein separation size and distribution analysis. |
| Methylene Blue | Used for DNA electrophoresis, particularly for post-staining post-electrophoresis. | Blue | Migrates slowly, suitable for larger DNA molecule analysis. |
| Nitro Blue Tetrazolium | Used for enzyme activity detection post-protein electrophoresis. | Colorless (turns purple after reaction) | Mainly used for post-enzymatic activity detection, color change occurs after substrate interaction to detect enzyme activity. |
| Ethidium Bromide | Used for DNA gel electrophoresis. | Colorless or Pale Yellow (appears red fluorescence in gel) | Highly mutagenic, use with caution. |
| Acridine Orange | Used as a DNA dye, also for RNA or protein electrophoresis sample loading dye. | Green or Red | This dye binds to nucleic acids and emits fluorescence, requiring fluorescent detection equipment. |
| Bromocresol Blue | Commonly used for DNA gel electrophoresis, especially as a DNA sample tracking dye. | Blue | Migrates slowly, suitable for tracking high molecular weight DNA. |
Professional Gel Electrophoresis Dye Customization Services
In biological science and analytical chemistry research, standardized gel electrophoresis dyes may not meet all experimental needs. Different laboratories, research fields, and even specific experimental designs may require optimization for specific samples, detection methods, or experimental conditions. Therefore, BOC Sciences offers professional customized gel electrophoresis dye services to meet customers' individual needs and ensure optimal experimental results.
Component and Formula Optimization
Adjusting the dye's components and ratios according to customer requirements to optimize staining effects. For example, improving DNA/RNA detection sensitivity, enhancing protein staining affinity, and adjusting the dye formula in buffer systems to ensure maximum experimental effectiveness.
Concentration and Viscosity Optimization
We customize dyes with different concentrations and viscosities based on experimental needs. High-concentration dyes are suitable for reducing sample volume, while low concentrations are ideal for long electrophoresis durations. Viscosity adjustments ensure uniform sample distribution and enhance experimental repeatability.
Spectral Property Adjustment
Providing a variety of fluorescent dye options, supporting excitation and emission at different wavelengths. Custom red, green, blue, and near-infrared dyes meet experimental laser scanning and imaging needs, enhancing signals and supporting multicolor experiments.
Special Labeling and Functional Modifications
We offer special labeling and functional modifications, such as biotinylation, radioactive labeling, and photosensitive dyes, to enhance the functionality of the dyes, suitable for various special experimental needs such as Western Blot, photo-crosslinking experiments, and low-concentration detection.
One-Stop Development Solutions for Custom Gel Staining Kits
With strong expertise in chemistry and biotechnology research and development, BOC Sciences provides one-stop solutions for gel staining kits to research institutions and industrial users. Our well-designed kits cover DNA, RNA, and protein gel electrophoresis staining, including conventional dyes, fluorescent dyes, and high-sensitivity dyes to meet various research needs. Our products are rigorously optimized to ensure a high signal-to-noise ratio, low background interference, and excellent stability, suitable for both agarose and polyacrylamide gels. In addition to standardized kits, we also offer custom formulations, special buffer solutions, and optimized staining protocols to help researchers achieve high-resolution experimental results. Furthermore, our kits come with detailed operation guides and technical support, ensuring stable and repeatable results during experiments, improving experimental efficiency, and accelerating research progress.
Strict Quality Control System Ensures Products Meet Industry Standards
Ensuring the high quality and consistency of products is crucial in the development and production of gel electrophoresis dyes. BOC Sciences adopts a strict quality control system, combined with advanced analytical instruments and testing equipment, to ensure that each batch of dye products meets industry standards and satisfies customer experimental needs. Our quality management covers various stages, including raw material selection, production process optimization, quality testing, packaging, and storage, ensuring the stability, safety, and reliability of the dyes.
- Raw Material Selection: All chemical reagents used in dyes undergo high-purity screening to ensure they are free from impurities or contaminants, preventing interference in experiments.
- Production Process Monitoring: Standardized production processes and GM P quality management are applied to ensure consistency and stability of each batch of products.
- Batch Consistency Testing: We perform rigorous quality comparison tests on different batches of dyes to ensure product stability and performance consistency under varying time and production conditions.
- Stability Testing: Simulating long-term storage conditions (such as different temperatures, humidity, and light conditions) to test the degradation of dyes in various storage environments, ensuring optimal performance during shelf life.
- Dye Spectral Properties: Testing the maximum absorption peak and maximum emission peak to ensure strong and stable fluorescent signal intensity.
- Migration Stability: Ensuring that dyes maintain consistent electrophoretic migration patterns under different buffer systems, electric field strengths, and gel concentrations.
- Background Signal Intensity: Measuring the fluorescent background of dyes when not bound to samples, ensuring low background interference to improve the signal-to-noise ratio.
- Sample Compatibility: Testing the staining effects on various nucleic acid and protein samples in electrophoresis experiments to ensure the dye's applicability to a wide range of research applications.
- Storage Stability: Testing the degradation rate of dyes under different temperature conditions (such as 4 °C, -20 °C) to ensure long-term stability during use.
- Biosafety Regulations: Ensuring that all biologically-related dyes are safe and free from carcinogenic, mutagenic, or reproductive toxic components.
Advanced Analytical Platform
- UV-Vis Spectrophotometer
- High-Performance Liquid Chromatography
- Fluorescence Spectrometer
- Mass Spectrometry
- Gel Electrophoresis Imaging System
- Spectrophotometer
- Viscometer
- Thin Layer Chromatography
Applications of Gel Electrophoresis in Modern Biotechnology Research
Gel electrophoresis is a widely used molecular separation technique in life sciences and biotechnology research, applied to the analysis, characterization, and quantification of nucleic acids and proteins. With its high resolution and reliability, gel electrophoresis plays an essential role in various fields, including genomics, proteomics, biomedical research, food science, forensic science, environmental science, and the biotechnology industry. The following are its primary applications in different research areas:
Genomics and DNA Analysis
In genomics research, gel electrophoresis is a core tool for DNA fragment analysis and identification. Additionally, it is used for DNA restriction enzyme mapping, gene typing, mutation detection, and DNA fingerprinting. With advancements in fluorescence dyes and high-sensitivity detection techniques, gel electrophoresis combined with imaging systems can accurately analyze low-abundance DNA, supporting human genome research, genetic disease studies, and DNA sequencing technologies.
RNA Research and Transcriptomics
In RNA analysis, gel electrophoresis is used to assess the integrity and degradation of total RNA, mRNA, or small RNAs (e.g., miRNA). Especially before RNA-seq studies, sample quality control is crucial, and electrophoresis can be used to check if the rRNA 28S and 18S bands are clear to evaluate degradation levels. Additionally, gel electrophoresis is used in Northern blot experiments to separate specific RNA fragments, coupled with probe hybridization techniques for gene expression analysis.
Proteomics and Protein Analysis
In proteomics research, SDS-PAGE (sodium dodecyl sulfate-polyacrylamide gel electrophoresis) is one of the most commonly used protein separation methods, distinguishing different proteins based on molecular weight. This technique is crucial in protein purification, protein expression analysis, Western blotting, and protein complex research. Furthermore, two-dimensional gel electrophoresis (2D-GE), combining isoelectric focusing (IEF) and SDS-PAGE, can separate proteins with different isoelectric points and molecular weights, often used in protein identification, post-translational modification analysis, and disease-related protein screening.
Biomedical and Disease Diagnosis
Gel electrophoresis plays a vital role in medical diagnostics and disease research. For example, in genetic disease diagnosis, electrophoresis can detect DNA fragment size changes caused by mutations, such as in sickle cell anemia and cystic fibrosis. Additionally, protein electrophoresis techniques, like isoelectric focusing (IEF), are used to analyze serum protein patterns, aiding in the diagnosis of autoimmune diseases, plasma protein abnormalities, and multiple myeloma.
Food Science and Food Safety Testing
Gel electrophoresis technology is used in food science for ingredient identification and quality control. For example, in genetically modified food detection, PCR combined with gel electrophoresis can identify specific gene sequences, distinguishing genetically modified from non-genetically modified foods. Additionally, protein electrophoresis is used to analyze protein compositions in dairy products, meats, and cereals to ensure food quality and purity.
Biotechnology and Synthetic Biology
In the biotechnology industry, gel electrophoresis is used for quality control of nucleic acid and protein products. For example, in genetic engineering, electrophoresis is used to confirm the successful construction of cloning vectors, ensuring high success rates in gene editing experiments. In recombinant protein production, SDS-PAGE combined with Western blotting can be used to detect protein expression levels and purity. Additionally, gel electrophoresis is used in synthetic biology research for DNA assembly validation, such as CRISP R vector construction, DNA fragment ligation, and plasmid screening, ensuring correct design of genetic circuits.
FAQs About Gel Electrophoresis
How does gel electrophoresis work?
Gel electrophoresis is a technique that separates charged molecules (such as DNA, RNA, or proteins) using an electric field. Samples are loaded into gel wells, and under the influence of an electric field, molecules migrate through the gel based on their charge, size, and shape. Smaller molecules migrate faster, while larger molecules move more slowly, achieving molecular separation. Finally, the distribution of molecules in the gel is observed through dyes or other detection methods to complete the analysis.
What is gel electrophoresis used for?
Gel electrophoresis is widely used in biological, chemical, and medical research to separate and analyze DNA, RNA, and proteins. It is applied in genomics, genotyping, protein expression studies, nucleic acid identification, and mutation detection. It is also commonly used in molecular cloning, PCR product analysis, and protein purification and analysis.
What is the gel in gel electrophoresis?
The gel in gel electrophoresis is typically made of agarose or polyacrylamide and serves as a solid medium with a network-like structure. Its main role is to provide a medium through which molecules migrate based on their size and shape under the electric field. Agarose gels are used for DNA and RNA separation, while polyacrylamide gels are used for protein separation.
How to make tbe buffer for gel electrophoresis?
TBE buffer typically consists of Tris (Tris(hydroxymethyl)aminomethane), boric acid, and EDTA (ethylenediaminetetraacetic acid). A common formulation is 89 mM Tris, 89 mM boric acid, and 2 mM EDTA. To prepare, dissolve Tris and boric acid in deionized water, add EDTA, adjust the pH to 8.0, and then dilute to the final volume with deionized water. This buffer is widely used for DNA and RNA electrophoresis.
Online Inquiry
