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5-Aminofluorescein | 3326-34-9
| Catalog Number | F04-0033 |
| Category | Fluorescein FAM |
| Molecular Formula | C20H13NO5 |
| Molecular Weight | 347.32 |
| Catalog Number | Size | Price | Quantity |
|---|---|---|---|
| F04-0033 | 5 g | $199 |
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Product Introduction
Fluoresceinamine isomer I is a building block for synthesis of fluorogenic form of flourescein.
Chemical Information |
|
|---|---|
| Synonyms | 5-Aminofluorescein (isomer I);5-Aminofluorescein |
| Purity | 98.0% |
| IUPAC Name | 6-amino-3',6'-dihydroxyspiro[2-benzofuran-3,9'-xanthene]-1-one |
| Canonical SMILES | C1=CC2=C(C=C1N)C(=O)OC23C4=C(C=C(C=C4)O)OC5=C3C=CC(=C5)O |
| InChI | InChI=1S/C20H13NO5/c21-10-1-4-14-13(7-10)19(24)26-20(14)15-5-2-11(22)8-17(15)25-18-9-12(23)3-6-16(18)20/h1-9,22-23H,21H2 |
| InChI Key | GZAJOEGTZDUSKS-UHFFFAOYSA-N |
| Appearance | Brown to Dark Brown Solid |
| MDL Number | MFCD00005052 |
- Product Specification
- Application
| Condition To Avoid | Light Sensitive |
| Signal | Warning |
| GHS Hazard Statements | H302 (98.81%): Harmful if swallowed [Warning Acute toxicity, oral] H315 (100%): Causes skin irritation [Warning Skin corrosion/irritation] H319 (100%): Causes serious eye irritation [Warning Serious eye damage/eye irritation] H335 (100%): May cause respiratory irritation [Warning Specific target organ toxicity, single exposure; Respiratory tract irritation] |
| Precautionary Statement Codes | P261, P264, P264+P265, P270, P271, P280, P301+P317, P302+P352, P304+P340, P305+P351+P338, P319, P321, P330, P332+P317, P337+P317, P362+P364, P403+P233, P405, and P501 (The corresponding statement to each P-code can be found at the GHS Classification page.) |
5-Aminofluorescein is a fluorescent dye with various applications in biological research and medical diagnostics. Here are some key applications of 5-Aminofluorescein:
Fluorescence Microscopy: 5-Aminofluorescein is widely used as a fluorescent marker in fluorescence microscopy to visualize cellular components and structures. It provides high sensitivity and specificity, allowing researchers to observe biological processes in real-time. This application is crucial for studying cell biology, tissue architecture, and intracellular dynamics.
Flow Cytometry: In flow cytometry, 5-Aminofluorescein is employed to label and quantify specific cell populations in a heterogeneous sample. Its bright fluorescence enables the detection and analysis of antigen expression, cell viability, and various cellular functions. This technique is essential for immunophenotyping, cancer research, and monitoring immune responses.
Immunofluorescence: 5-Aminofluorescein can be conjugated to antibodies or other biomolecules for use in immunofluorescence assays. This application allows for the precise localization of target proteins, antigens, or cellular structures within tissue sections or cell cultures. It is widely utilized in diagnostics, pathology, and basic research to study protein distribution and cellular signaling pathways.
Molecular Diagnostics: 5-Aminofluorescein is used in molecular diagnostic assays, such as fluorescence in situ hybridization (FISH). It labels probes that hybridize to specific DNA or RNA sequences, enabling the detection of genetic abnormalities or infections. This application is valuable in clinical diagnostics for identifying genetic disorders, infectious diseases, and cancer biomarkers.
Computed Properties | |
|---|---|
| XLogP3 | 2.7 |
| Hydrogen Bond Donor Count | 3 |
| Hydrogen Bond Acceptor Count | 6 |
| Rotatable Bond Count | 0 |
| Exact Mass | 347.07937252 g/mol |
| Monoisotopic Mass | 347.07937252 g/mol |
| Topological Polar Surface Area | 102Ų |
| Heavy Atom Count | 26 |
| Formal Charge | 0 |
| Complexity | 557 |
| Isotope Atom Count | 0 |
| Defined Atom Stereocenter Count | 0 |
| Undefined Atom Stereocenter Count | 0 |
| Defined Bond Stereocenter Count | 0 |
| Undefined Bond Stereocenter Count | 0 |
| Covalently-Bonded Unit Count | 1 |
| Compound Is Canonicalized | Yes |
Literatures
| PMID | Publication Date | Title | Journal |
|---|---|---|---|
| 22477061 | 2012-07-01 | Reduced fluoresceinamine for peroxynitrite quantification in the presence of nitric oxide | Journal of fluorescence |
| 22293239 | 2012-03-02 | Intracellular protein delivery by hollow mesoporous silica capsules with a large surface hole | Nanotechnology |
| 22057128 | 2012-02-18 | Ratiometric fluorescence imaging for distinguishing chloride concentration between normal and ischemic ventricular myocytes | Chemical communications (Cambridge, England) |
| 22014935 | 2012-02-01 | Surface modification of cell culture carriers: routes to anhydride functionalization of polystyrene | Colloids and surfaces. B, Biointerfaces |
| 21936055 | 2011-11-01 | Preparation and characterization of affinity sorbents based on isoalloxazine-like ligands for separation of flavoenzymes | Journal of separation science |
Patents
| Publication Number | Title | Priority Date |
|---|---|---|
| CN-113880821-A | Design and synthesis method of fluorescent probe for imaging epileptic intracerebral hypochlorous acid characteristics by two-photon fluorescent probe | 2021-10-29 |
| CN-113984749-A | Electrostatic spray spinning nanofiber test paper and preparation method and application thereof | 2021-10-26 |
| CN-113984749-B | A kind of electrostatic spray spinning nanofiber test paper and its preparation method and application | 2021-10-26 |
| CN-113667755-A | Biomarkers associated with oxaliplatin resistance | 2021-09-29 |
| CN-113684278-A | Biomarkers for predicting colorectal cancer sensitivity to oxaliplatin therapy and their applications | 2021-09-29 |
Applications of Fluorescent Probes & Dyes
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