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Fluorescent Dyes
Fluorescein FAM
FAM Amine

What We Offer

FAM Amine

Name: FAM Amine
Brand: BOC Sciences
Rating: 5 (1 reviews)

Catalog Number	F04-0042
Category	Fluorescein FAM
Molecular Weight	420

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Product Introduction

FAM Amine is inactive form of bright green fluorescent dye used to generate a stable fluorescence signal in bioimaging. The maxima of Ex/Em values are at 494/520 nm, similar to that of Alexa 488 and Cy2. FAM might be excited using 488 nm laser line and displays good optical property. The primary amine is attached to the fluorophore through an ethylamino linkage. FAM amine can be coupled with carboxylic acids at small molecules or on biomolecules by standard amide bond coupling conditions or can be utilized as a reference standard for dye-conjugates.

Chemical Information
Product Specification
Application

Solubility	DMF, DMSO
Appearance	Yellow solid

CF280	0.2
Excitation	494
Emission	520
Storage	4 °C, protect from light

FAM Amine, a fluorescent dye prevalent in molecular biology and biochemistry, finds diverse applications across various scientific realms. Here are four key applications of FAM Amine:

Fluorescent Labeling: Widely embraced for its vibrant green fluorescence, FAM Amine serves as a versatile tool for tagging nucleic acids, proteins, and other biomolecules in an array of fluorescence-based assays. This dye’s luminous properties make it particularly well-suited for applications like fluorescence in situ hybridization (FISH) and flow cytometry. Moreover, its exceptional photostability and seamless integration with automated detection systems render FAM Amine a preferred choice for both quantitative and qualitative studies, illuminating the microscopic world with precision.

Real-time PCR: In the realm of quantitative PCR (qPCR), FAM Amine emerges as a pivotal player, serving as a beacon in TaqMan probes. The dye’s fluorescence enables real-time monitoring of DNA amplification, facilitating accurate quantification of target sequences. This technique holds immense significance in clinical diagnostics, pathogen detection, and gene expression analysis.

Protein Interaction Studies: Delving into the intricate realm of protein-protein interactions and cellular dynamics, FAM Amine emerges as a valuable asset for tagging proteins in fluorescence microscopy studies. By attaching FAM Amine to specific proteins, researchers gain insights into their whereabouts and interaction dynamics within live cells.

High-Throughput Screening: Empowering high-throughput screening (HTS) assays with its fluorescent prowess, FAM Amine-labeled compounds catalyze the identification of potential drug candidates with remarkable speed and sensitivity. The fluorescent signal emanating from FAM Amine facilitates swift and precise detection of biochemical and cellular activities within vast compound libraries, propelling the drug discovery process forward and unearthing novel therapeutic avenues.