![{[Name]}](https://resource.bocsci.com/structure/76823-03-5.gif?v=2025011501)
![{[Name]}](https://resource.bocsci.com/structure/76-54-0.gif?v=2025011501)
![{[Name]}](https://resource.bocsci.com/structure/51649-83-3.gif?v=2025011501)
![{[Name]}](https://resource.bocsci.com/structure/147566-42-5.gif?v=2025011501)
![{[Name]}](https://resource.bocsci.com/structure/192374-17-7.gif?v=2025011501)
![{[Name]}](https://resource.bocsci.com/structure/92557-80-7.gif?v=2025011501)
Inquiry Basket
5-Carboxyfluorescein diacetate | CAS 79955-27-4
| Catalog Number | F04-0034 |
| Category | Fluorescein FAM |
| Molecular Formula | C25H16O9 |
| Molecular Weight | 460.394 |
| Catalog Number | Size | Price | Quantity |
|---|---|---|---|
| F04-0034 | 500 mg | $228 |
* Please be kindly noted products are not for therapeutic use. We do not sell to patients.
Product Introduction
5-Carboxyfluorescein diacetate is a non-fluorescent, cell-permeant hydrophobic probe that is used in the synthesis of rhodamines.
Chemical Information
Product Specification
Application
Computed Properties
Literatures
Patents
| Synonyms | 5-CFDA |
| Purity | >93.0%(T)(HPLC) |
| IUPAC Name | 3',6'-diacetyloxy-3-oxospiro[2-benzofuran-1,9'-xanthene]-5-carboxylic acid |
| Canonical SMILES | CC(=O)OC1=CC2=C(C=C1)C3(C4=C(C=C(C=C4)C(=O)O)C(=O)O3)C5=C(O2)C=C(C=C5)OC(=O)C |
| InChI | InChI=1S/C25H16O9/c1-12(26)31-15-4-7-19-21(10-15)33-22-11-16(32-13(2)27)5-8-20(22)25(19)18-6-3-14(23(28)29)9-17(18)24(30)34-25/h3-11H,1-2H3,(H,28,29) |
| InChIKey | WPUZGNPQMIWOHE-UHFFFAOYSA-N |
| Appearance | Solid |
| MDL Number | MFCD00036872 |
| Condition To Avoid | Heat Sensitive |
| Storage | <0 °C |
| GHSHazardStatements | Not Classified Reported as not meeting GHS hazard criteria by 38 of 39 companies (only ~ 2.6% companies provided GHS information). For more detailed information, please visit ECHA C&L website. |
5-Carboxyfluorescein diacetate (CFDA) stands out as a fluorescent dye with widespread use in diverse biological applications, thanks to its unique capability to penetrate cells and emit fluorescence upon enzymatic conversion. Here are four key applications of 5-Carboxyfluorescein diacetate:
Cell Viability Assays: CFDA plays a pivotal role in cell viability assays by assessing the viability of cells within a population. Once inside the cell, esterases transform CFDA into carboxyfluorescein, a highly fluorescent compound that accumulates in viable cells. This method enables researchers to efficiently differentiate between live and dead cells, aiding in toxicology studies and drug screening by providing crucial insights into cellular health and responses.
Flow Cytometry: Widely employed in flow cytometry, CFDA facilitates the study of cell populations and monitoring of cell proliferation dynamics. Cells labeled with CFDA emit fluorescence that can be quantitatively measured using flow cytometers, offering detailed information on cell cycle status and division rates. This application is particularly valuable in immunology research and cancer cell analysis, shedding light on cellular behaviors and interactions with precision and depth.
Tracking Cell Migration: CFDA emerges as a valuable tool for labeling and tracing cell migration patterns in various biological studies. Once labeled, cells retain their fluorescence, allowing for real-time visualization and tracking of their movements over time using fluorescence microscopy. This technique is pivotal for unraveling complex processes such as tissue regeneration, immune cell trafficking, and cancer metastasis, providing insights into the dynamics and mechanisms governing cell movements in diverse biological contexts.
Intracellular pH Measurement: Leveraging its pH-sensitive fluorescence properties, CFDA serves as a reliable option for measuring intracellular pH in living cells. Changes in fluorescence intensity of CFDA-loaded cells correspond to fluctuations in intracellular pH, offering valuable insights into cellular metabolic states and responses to environmental cues. This application is critical for advancing research in cell physiology and pathophysiology, enabling scientists to unravel the intricacies of cellular processes and adaptations to varying conditions with precision and depth.
| XLogP3 | 3.2 |
| Hydrogen Bond Donor Count | 1 |
| Hydrogen Bond Acceptor Count | 9 |
| Rotatable Bond Count | 5 |
| Exact Mass | 460.07943208 g/mol |
| Monoisotopic Mass | 460.07943208 g/mol |
| Topological Polar Surface Area | 125Ų |
| Heavy Atom Count | 34 |
| Formal Charge | 0 |
| Complexity | 828 |
| Isotope Atom Count | 0 |
| Defined Atom Stereocenter Count | 0 |
| Undefined Atom Stereocenter Count | 0 |
| Defined Bond Stereocenter Count | 0 |
| Undefined Bond Stereocenter Count | 0 |
| Covalently-Bonded Unit Count | 1 |
| Compound Is Canonicalized | Yes |
| PMID | Publication Date | Title | Journal |
|---|---|---|---|
| 22541068 | 2012-05-24 | Classification of inhibitors of hepatic organic anion transporting polypeptides (OATPs): influence of protein expression on drug-drug interactions | Journal of medicinal chemistry |
| 22558349 | 2012-01-01 | Regulation of epithelial cell morphology and functions approaching to more in vivo-like by modifying polyethylene glycol on polysulfone membranes | PloS one |
| 22027652 | 2011-12-01 | Functional defect caused by the 4544G>A SNP in ABCC2: potential impact for drug cellular disposition | Pharmacogenetics and genomics |
| 21609321 | 2011-08-01 | Function of MRP1/ABCC1 is not dependent on cholesterol or cholesterol-stabilized lipid rafts | The Biochemical journal |
| 20880209 | 2011-01-01 | Development of a nondestructive fluorescence-based enzymatic staining of microcolonies for enumerating bacterial contamination in filterable products | Journal of applied microbiology |
| Publication Number | Title | Priority Date |
|---|---|---|
| KR-20220158291-A | A pharmaceutical composition containing cell-transducing Inorganic Pyrophosphatase 1 fusion protein for preventing or treating motor neuronal disorder | 2021-05-20 |
| KR-20220147167-A | Pharmaceutical composition for treating cerebral ischemia containing cell-transducing PGAM5 fusion protein | 2021-04-26 |
| KR-20220142557-A | Pharmaceutical composition for treating cerebral ischemia containing cell-transducing CRYM fusion protein | 2021-04-14 |
| JP-2022037654-A | Compounds localized in the Golgi apparatus and their use | 2020-08-25 |
| JP-2021138671-A | PPM1D inhibitor | 2020-03-09 |
Recommended Services
Recommended Articles
- Hoechst Dyes: Definition, Structure, Mechanism and Applications
- Mastering the Spectrum: A Comprehensive Guide to Cy3 and Cy5 Dyes
- Fluorescent Probes: Definition, Structure, Types and Application
- Fluorescent Dyes: Definition, Mechanism, Types and Application
- Coumarin Dyes: Definition, Structure, Benefits, Synthesis and Uses
- BODIPY Dyes: Definition, Structure, Synthesis and Uses
- Cyanine Dyes: Definition, Structure, Types and Uses
- Fluorescein Dyes: Definition, Structure, Synthesis and Uses
- Rhodamine Dyes: Definition, Structure, Uses, Excitation and Emission
- Unlocking the Power of Fluorescence Imaging: A Comprehensive Guide
- Cell Imaging: Definitions, Systems, Protocols, Dyes, and Applications
- Lipid Staining: Definition, Principles, Methods, Dyes, and Uses
- Flow Cytometry: Definition, Principles, Protocols, Dyes, and Uses
- Nucleic Acid Staining: Definition, Principles, Dyes, Procedures, and Uses
- DNA Staining: Definition, Procedures, Benefits, Dyes and Uses
- Cell Staining: Definition, Principles, Protocols, Dyes, and Uses
- Ion Imaging: Definition, Principles, Benefits, Dyes, and Uses
- Fluorescent Labeling: Definition, Principles, Types and Applications
Recommended Products
Online Inquiry
