5-Carboxyfluorescein diacetate | CAS 79955-27-4

5-Carboxyfluorescein diacetate (CFDA) stands out as a fluorescent dye with widespread use in diverse biological applications, thanks to its unique capability to penetrate cells and emit fluorescence upon enzymatic conversion. Here are four key applications of 5-Carboxyfluorescein diacetate:

Cell Viability Assays: CFDA plays a pivotal role in cell viability assays by assessing the viability of cells within a population. Once inside the cell, esterases transform CFDA into carboxyfluorescein, a highly fluorescent compound that accumulates in viable cells. This method enables researchers to efficiently differentiate between live and dead cells, aiding in toxicology studies and drug screening by providing crucial insights into cellular health and responses.

Flow Cytometry: Widely employed in flow cytometry, CFDA facilitates the study of cell populations and monitoring of cell proliferation dynamics. Cells labeled with CFDA emit fluorescence that can be quantitatively measured using flow cytometers, offering detailed information on cell cycle status and division rates. This application is particularly valuable in immunology research and cancer cell analysis, shedding light on cellular behaviors and interactions with precision and depth.

Tracking Cell Migration: CFDA emerges as a valuable tool for labeling and tracing cell migration patterns in various biological studies. Once labeled, cells retain their fluorescence, allowing for real-time visualization and tracking of their movements over time using fluorescence microscopy. This technique is pivotal for unraveling complex processes such as tissue regeneration, immune cell trafficking, and cancer metastasis, providing insights into the dynamics and mechanisms governing cell movements in diverse biological contexts.

Intracellular pH Measurement: Leveraging its pH-sensitive fluorescence properties, CFDA serves as a reliable option for measuring intracellular pH in living cells. Changes in fluorescence intensity of CFDA-loaded cells correspond to fluctuations in intracellular pH, offering valuable insights into cellular metabolic states and responses to environmental cues. This application is critical for advancing research in cell physiology and pathophysiology, enabling scientists to unravel the intricacies of cellular processes and adaptations to varying conditions with precision and depth.