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Product Introduction
FAM amine, 5-isomer is an exceptional fluorescent probe deployed within the realm of biomedicine. It finds applicability in unraveling intricate cellular functionalities and protein-protein interplays. Its adeptness in labeling peptides, proteins, and nucleic acids is well-established. By imparting exceptional sensitivity and negating background fluorescence, the FAM amine, 5-isomer stands as an optimal choice for cutting-edge methodologies encompassing immunohistochemistry, flow cytometry, and in situ hybridization.
Chemical Information
Product Specification
Computed Properties
Related CAS |
2183440-41-5 (hydrochloride)
|
Synonyms |
6-isomer FAM amine; Fluorescein (FAM) amine |
Purity |
NMR 1H, HPLC-MS (95%) |
IUPAC Name |
6-[(3',6'-dihydroxy-3-oxospiro[2-benzofuran-1,9'-xanthene]-5-carbonyl)amino]hexylazanium;chloride |
Canonical SMILES |
C1=CC2=C(C=C1C(=O)NCCCCCC[NH3+])C(=O)OC23C4=C(C=C(C=C4)O)OC5=C3C=CC(=C5)O.[Cl-] |
InChI |
InChI=1S/C27H26N2O6.ClH/c28-11-3-1-2-4-12-29-25(32)16-5-8-20-19(13-16)26(33)35-27(20)21-9-6-17(30)14-23(21)34-24-15-18(31)7-10-22(24)27;/h5-10,13-15,30-31H,1-4,11-12,28H2,(H,29,32);1H |
InChIKey |
YMNCSJRUUHHNGI-UHFFFAOYSA-N |
Solubility |
good in methanol, DMSO, DMF |
Appearance |
yellow solid |
ε, L⋅mol-1⋅cm-1 |
80000 |
Fluorescence Quantum Yield |
0.93 |
Excitation |
490 |
Emission |
513 |
Storage |
24 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. Desiccate. |
Hydrogen Bond Donor Count |
4 |
Hydrogen Bond Acceptor Count |
7 |
Rotatable Bond Count |
7 |
Exact Mass |
510.1557643 g/mol |
Monoisotopic Mass |
510.1557643 g/mol |
Topological Polar Surface Area |
133Ų |
Heavy Atom Count |
36 |
Formal Charge |
0 |
Complexity |
751 |
Isotope Atom Count |
0 |
Defined Atom Stereocenter Count |
0 |
Undefined Atom Stereocenter Count |
0 |
Defined Bond Stereocenter Count |
0 |
Undefined Bond Stereocenter Count |
0 |
Covalently-Bonded Unit Count |
2 |
Compound Is Canonicalized |
Yes |