FAM amine, 5-isomer | 138589-19-2

Product Introduction

FAM amine, 5-isomer is an exceptional fluorescent probe deployed within the realm of biomedicine. It finds applicability in unraveling intricate cellular functionalities and protein-protein interplays. Its adeptness in labeling peptides, proteins, and nucleic acids is well-established. By imparting exceptional sensitivity and negating background fluorescence, the FAM amine, 5-isomer stands as an optimal choice for cutting-edge methodologies encompassing immunohistochemistry, flow cytometry, and in situ hybridization.

Chemical Information
Related CAS	2183440-41-5 (hydrochloride)
Synonyms	6-isomer FAM amine; Fluorescein (FAM) amine
Purity	NMR 1H, HPLC-MS (95%)
IUPAC Name	6-[(3',6'-dihydroxy-3-oxospiro[2-benzofuran-1,9'-xanthene]-5-carbonyl)amino]hexylazanium;chloride
Canonical SMILES	C1=CC2=C(C=C1C(=O)NCCCCCC[NH3+])C(=O)OC23C4=C(C=C(C=C4)O)OC5=C3C=CC(=C5)O.[Cl-]
InChI	InChI=1S/C27H26N2O6.ClH/c28-11-3-1-2-4-12-29-25(32)16-5-8-20-19(13-16)26(33)35-27(20)21-9-6-17(30)14-23(21)34-24-15-18(31)7-10-22(24)27;/h5-10,13-15,30-31H,1-4,11-12,28H2,(H,29,32);1H
InChI Key	YMNCSJRUUHHNGI-UHFFFAOYSA-N
Solubility	good in methanol, DMSO, DMF
Appearance	yellow solid

Product Specification
ε, L⋅mol-1⋅cm-1	80000
Fluorescence Quantum Yield	0.93
Excitation	490
Emission	513
Storage	24 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. Desiccate.

Computed Properties
Hydrogen Bond Donor Count	4
Hydrogen Bond Acceptor Count	7
Rotatable Bond Count	7
Exact Mass	510.1557643 g/mol
Monoisotopic Mass	510.1557643 g/mol
Topological Polar Surface Area	133Ų
Heavy Atom Count	36
Formal Charge	0
Complexity	751
Isotope Atom Count	0
Defined Atom Stereocenter Count	0
Undefined Atom Stereocenter Count	0
Defined Bond Stereocenter Count	0
Undefined Bond Stereocenter Count	0
Covalently-Bonded Unit Count	2
Compound Is Canonicalized	Yes