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DAPI dihydrochloride | 28718-90-3
| Catalog Number | A16-0201 |
| Category | Nuclear Fluorescent Probes |
| Molecular Formula | C16H15N5·2HCl |
| Molecular Weight | 350.3 |
| Catalog Number | Size | Price | Quantity |
|---|---|---|---|
| A16-0201 | 250 mg | $439 |
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Product Introduction
DAPI is a fluorescent DNA stain that binds to the minor groove of double-stranded DNA with preference for adenine and thymine (AT) rich DNA. DAPI is commonly used in microscopy and flow cytometry. It can be used on either fixed or live cells, although it crosses membranes in live cells less efficiently and demands higher concentrations to be used.
Chemical Information |
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|---|---|
| Related CAS | 47165-04-8 (free base) |
| Synonyms | DAPI;2-[4-(aminoiminomethyl)phenyl]-1H-indole-6-carboximidamide dihydrochloride |
| Purity | ≥90% |
| Canonical SMILES | C1=CC(=CC=C1C2=CC3=C(N2)C=C(C=C3)C(=N)N)C(=N)N.Cl.Cl |
| InChI | InChI=1S/C16H15N5.2ClH/c17-15(18)10-3-1-9(2-4-10)13-7-11-5-6-12(16(19)20)8-14(11)21-13;;/h1-8,21H,(H3,17,18)(H3,19,20);2*1H |
| InChI Key | FPNZBYLXNYPRLR-UHFFFAOYSA-N |
| Solubility | H2O |
| Density | 0.921 g/mL at 25 °C (lit.) |
| Appearance | Yellow Solid |
| Refractive Index | n20/D 1.498 (lit.) |
| Melting Point | >300°C (dec.) |
| MDL Number | MFCD00042988 |
| NACRES | NA.52 |
- Product Specification
- Application
| Excitation | 358 nm |
| Emission | 461 nm |
| Properties Quality Level | 300 |
| Storage | Store at -20°C |
DAPI dihydrochloride, a fluorescent stain, binds strongly to DNA, making it an essential tool in various bioscience applications. Here are some key applications of DAPI dihydrochloride:
Cellular Imaging: DAPI dihydrochloride is widely used to stain cell nuclei in fluorescence microscopy. By binding to the minor groove of A-T rich regions of DNA, it helps researchers visualize and quantify nuclear DNA. This technique is crucial for studying cell cycle dynamics, apoptosis, and chromosomal arrangements.
Flow Cytometry: In flow cytometry, DAPI dihydrochloride is used to assess cell viability and DNA content. By staining cells, it enables differentiation between live and dead cells based on membrane permeability. Additionally, it is used to measure DNA content for cell cycle analysis, providing insights into cellular proliferation and apoptosis.
Chromosome Staining: DAPI dihydrochloride is employed for the staining of chromosomes in karyotyping and fluorescence in situ hybridization (FISH) techniques. It helps in identifying chromosomal abnormalities and structural variations. This application is significant in clinical diagnostics, genetic research, and understanding genetic disorders.
Microbial Studies: DAPI dihydrochloride is used to enumerate and visualize bacteria and other microorganisms. By staining microbial DNA, it allows researchers to differentiate between bacterial species and assess microbial viability. This is essential for ecological studies, water quality testing, and microbiome analyses.
Applications of Fluorescent Probes & Dyes
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