Hoechst 33258 | CAS 23491-45-4

Catalog Number	A19-0041
Category	DNA Stains
Molecular Formula	C25H24N6O·3HCl
Molecular Weight	533.9

Inquiry

* Please be kindly noted products are not for therapeutic use. We do not sell to patients.

Worldwide Delivery
Quality Assurance
24/7 Customer Service

Product Introduction

Hoechst 33258 is a cell-permeant blue fluorescent dye for staining DNA in live or fixed cells. It’s widely used for nuclear visualization and cell cycle analysis.

Chemical Information
Product Specification
Computed Properties
Patents

Chemical Information

Synonyms	Pibenzimol Hydrochloride; HOE 33258; 4-[6-(4-methyl-1-piperazinyl)[2,6'-bi-1H-benzimidazol]-2'-yl]-phenol trihydrochloride
Purity	≥95%
IUPAC Name	4-[6-[6-(4-methylpiperazin-1-yl)-1H-benzimidazol-2-yl]-1H-benzimidazol-2-yl]phenol;trihydrochloride
SMILES	CN1CCN(CC1)C2=CC3=C(C=C2)N=C(N3)C4=CC5=C(C=C4)NC(=C6C=CC(=O)C=C6)N5.Cl.Cl.Cl
InChI	InChI=1S/C25H24N6O.3ClH/c1-30-10-12-31(13-11-30)18-5-9-21-23(15-18)29-25(27-21)17-4-8-20-22(14-17)28-24(26-20)16-2-6-19(32)7-3-16;;;/h2-9,14-15,32H,10-13H2,1H3,(H,26,28)(H,27,29);3*1H
InChIKey	SMNPLAKEGAEPJD-UHFFFAOYSA-N
Solubility	Soluble to 20 mM in DMSO and to 100 mM in water.
Appearance	Yellow Solid

Product Specification

Excitation	352 nm
Emission	461 nm
Storage	Store at -20°C
Signal	Warning
GHSHazardStatements	H302 (85.11%): Harmful if swallowed [Warning Acute toxicity, oral] H315 (100%): Causes skin irritation [Warning Skin corrosion/irritation] H319 (100%): Causes serious eye irritation [Warning Serious eye damage/eye irritation]
Precautionary Statement Codes	P264, P264+P265, P270, P280, P301+P317, P302+P352, P305+P351+P338, P321, P330, P332+P317, P337+P317, P362+P364, and P501 (The corresponding statement to each P-code can be found at the GHS Classification page.)

Computed Properties

Hydrogen Bond Donor Count	6
Hydrogen Bond Acceptor Count	5
Rotatable Bond Count	3
Exact Mass	532.131193 g/mol
Monoisotopic Mass	532.131193 g/mol
Topological Polar Surface Area	84.1Å²
Heavy Atom Count	35
Formal Charge	0
Complexity	634
Isotope Atom Count	0
Defined Atom Stereocenter Count	0
Undefined Atom Stereocenter Count	0
Defined Bond Stereocenter Count	0
Undefined Bond Stereocenter Count	0
Covalently-Bonded Unit Count	4
Compound Is Canonicalized	Yes

Patents

Publication Number	Title	Priority Date
WO-2022221852-A1	Multi-layer amniotic tissue grafts and uses thereof	2021-04-13
WO-2022159737-A1	Anti-kit antibodies and uses thereof	2021-01-22
WO-2022147463-A2	Binder molecules with high affinity and/ or specificity and methods of making and use thereof	2020-12-31
US-2021032355-A1	Methods of Treating Cytokine Storm Infections, Including COVID-19, By Inhibiting CCR5/CCL5 (RANTES) Interaction, and Compositions for Practicing the Same	2020-04-27
US-11180491-B2	Method of treating COVID-19 by administering the CCR5/CCL5 (RANTES) interaction inhibitor maraviroc	2020-04-27