6-Hexachloro-Fluorescein Phosphoramidite | CAS 1360547-55-2

6-Hexachloro-Fluorescein Phosphoramidite, a fluorescent labeling reagent extensively used in molecular biology and biochemistry, finds diverse applications in various scientific domains. Here are four key applications of this compound:

DNA Sequencing: Serving as a pivotal component in automated DNA sequencing methodologies, 6-Hexachloro-Fluorescein Phosphoramidite acts as a fluorescent dye, enabling the labeling of nucleotides for pinpoint detection and differentiation during sequencing procedures. Its exceptional sensitivity and precise emission wavelength render it an optimal choice for sequencing tasks, ensuring the meticulous interpretation of DNA sequences with unparalleled accuracy.

Fluorescence In Situ Hybridization (FISH): Employed in the technique of FISH, this compound plays a critical role in labeling DNA or RNA probes with fluorescent tags. Through this process, researchers can visualize the spatial distribution of specific genetic sequences within chromosomes or tissues, a fundamental practice for diagnosing genetic irregularities and investigating the intricate arrangement of chromosomal elements.

Oligonucleotide Synthesis: Integral to the synthesis of fluorescently-labeled oligonucleotides for diverse applications like molecular diagnostics, PCR, and real-time PCR, 6-Hexachloro-Fluorescein Phosphoramidite introduces a fluorescent marker that allows for the monitoring and quantification of oligonucleotides during amplification and detection phases. This integration of fluorescent labels enhances the sensitivity and specificity of molecular techniques, facilitating advanced analytical capabilities.

Protein-DNA Interaction Studies: Through the incorporation of 6-Hexachloro-Fluorescein Phosphoramidite into DNA sequences, researchers delve into the realm of protein-DNA interactions via fluorescence-based assays. These interactions play a crucial role in unraveling the complexities of gene regulation and transcriptional control mechanisms. The fluorescent tagging aids in observing binding events and determining binding affinities, shedding light on the intricate interplay between proteins and DNA molecules in biological processes.