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Sulfo-Cyanine3 dUTP
| Catalog Number | R11-0003 |
| Category | DNA Stains |
| Molecular Formula | C47H62N6Li4O22P3S2 |
| Molecular Weight | 1247.84 |
* Please be kindly noted products are not for therapeutic use. We do not sell to patients.
Product Introduction
Labeled deoxynucleoside triphosphates (dNTPs) provide an efficient way for the enzymatic labeling of DNA. DNA microarrays, PCR amplicons, and reverse transcripts can be labeled using various dNTPs.Sulfo-Cyanine3 is a dye emitting in yellow range. This fluorophore is a standard dye used in DNA microarray experiments. Sulfo-Cyanine3 dUTP is a labeled triphosphate providing efficient labeling in enzymatic reactions with various enzymes, including simple Taq polymerase. Quality of each batch is assured by HPLC-MS, and testing in enzymatic reaction.
Chemical Information
Product Specification
Application
Computed Properties
| Purity | HPLC-MS (95%), testing in enzymatic reaction |
| IUPAC Name | tetralithium;1-[6-[[6-[3-[1-[(2R,4S,5R)-4-hydroxy-5-[[[[hydroxy(oxido)phosphoryl]oxy-oxidophosphoryl]oxy-oxidophosphoryl]oxymethyl]oxolan-2-yl]-2,4-dioxopyrimidin-5-yl]prop-2-ynylamino]-6-oxohexyl]amino]-6-oxohexyl]-3,3-dimethyl-2-[3-(1,3,3-trimethyl-5-sulfonatoindol-1-ium-2-yl)prop-2-enylidene]indole-5-sulfonate |
| Canonical SMILES | [Li+].[Li+].[Li+].[Li+].CC1(C2=C(C=CC(=C2)S(=O)(=O)[O-])[N+](=C1C=CC=C3C(C4=C(N3CCCCCC(=O)NCCCCCC(=O)NCC#CC5=CN(C(=O)NC5=O)C6CC(C(O6)COP(=O)([O-])OP(=O)([O-])OP(=O)(O)[O-])O)C=CC(=C4)S(=O)(=O)[O-])(C)C)C)C |
| InChI | InChI=1S/C48H63N6O22P3S2.4Li/c1-47(2)34-26-32(80(67,68)69)19-21-36(34)52(5)40(47)15-12-16-41-48(3,4)35-27-33(81(70,71)72)20-22-37(35)53(41)25-11-7-9-18-42(56)49-23-10-6-8-17-43(57)50-24-13-14-31-29-54(46(59)51-45(31)58)44-28-38(55)39(74-44)30-73-78(63,64)76-79(65,66)75-77(60,61)62;;;;/h12,15-16,19-22,26-27,29,38-39,44,55H,6-11,17-18,23-25,28,30H2,1-5H3,(H8-,49,50,51,56,57,58,59,60,61,62,63,64,65,66,67,68,69,70,71,72);;;;/q;4*+1/p-4/t38-,39+,44+;;;;/m0..../s1 |
| InChIKey | RCMVJDZVTUKRMS-HBRXSBNGSA-J |
| Appearance | red solid / solution |
| ε, L⋅mol-1⋅cm-1 | 162000 |
| Fluorescence Quantum Yield | 0.1 |
| Excitation | 548 |
| Emission | 563 |
| Storage | 12 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. Desiccate. |
Sulfo-Cyanine3 dUTP is a fluorescent dye-labeled nucleotide commonly used in DNA staining applications due to its strong fluorescence and excellent photostability. One of its key applications is in fluorescence in situ hybridization (FISH) techniques. In FISH, Sulfo-Cyanine3 dUTP is incorporated into DNA probes, allowing the visualization of specific DNA sequences within chromosomes or tissues. This method is widely used in genetic research and diagnostics, enabling the detection of genetic abnormalities, gene mapping, and chromosomal rearrangements. The high brightness and stability of Sulfo-Cyanine3 ensure clear and accurate signal detection in these applications.
Another critical application of Sulfo-Cyanine3 dUTP is in quantitative real-time PCR (qPCR). During qPCR, the incorporation of Sulfo-Cyanine3 dUTP into DNA molecules allows for the precise quantification of target DNA sequences. The fluorescence intensity of Sulfo-Cyanine3 increases proportionally with the amount of amplified DNA, making it an ideal choice for monitoring the progress of PCR reactions in real-time. This application is crucial for gene expression analysis, pathogen detection, and genotyping studies, where accurate and sensitive detection of nucleic acids is required.
Sulfo-Cyanine3 dUTP is also used in single-cell sequencing and analysis. In this application, Sulfo-Cyanine3-labeled nucleotides are incorporated into the DNA of individual cells, enabling the tracking and analysis of specific genetic sequences within heterogeneous cell populations. This approach is vital for understanding cellular diversity, gene regulation, and the development of complex biological systems. The use of Sulfo-Cyanine3 dUTP in single-cell sequencing provides researchers with the ability to visualize and quantify gene expression patterns at the single-cell level, offering deeper insights into cellular functions and interactions.
Lastly, Sulfo-Cyanine3 dUTP is employed in DNA microarray technologies. DNA microarrays are powerful tools for analyzing gene expression profiles and genetic variations across large sets of genes. By incorporating Sulfo-Cyanine3 dUTP into DNA probes, researchers can generate highly fluorescent signals corresponding to specific genetic sequences on the microarray. This allows for high-throughput screening of genetic information, facilitating studies in genomics, pharmacogenomics, and personalized medicine. The use of Sulfo-Cyanine3 dUTP enhances the sensitivity and reliability of DNA microarray assays, making it an indispensable reagent in modern molecular biology research.
| Hydrogen Bond Donor Count | 5 |
| Hydrogen Bond Acceptor Count | 23 |
| Rotatable Bond Count | 24 |
| Exact Mass | 1256.2976846 g/mol |
| Monoisotopic Mass | 1256.2976846 g/mol |
| Topological Polar Surface Area | 443Ų |
| Heavy Atom Count | 85 |
| Formal Charge | 0 |
| Complexity | 2930 |
| Isotope Atom Count | 0 |
| Defined Atom Stereocenter Count | 3 |
| Undefined Atom Stereocenter Count | 0 |
| Defined Bond Stereocenter Count | 0 |
| Undefined Bond Stereocenter Count | 2 |
| Covalently-Bonded Unit Count | 5 |
| Compound Is Canonicalized | Yes |
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