Ethidium homodimer-1 | 61926-22-5

Ethidium homodimer-1, a potent fluorescent nucleic acid stain widely utilized in biological research for its strong DNA binding affinity, boasts a plethora of applications. Here are four key applications of Ethidium homodimer-1:

Live/Dead Cell Assays: A staple in live/dead cell viability assays, Ethidium homodimer-1 serves as a discriminating agent between viable and deceased cells. It selectively infiltrates the compromised membranes of dead cells, binding to DNA and emitting a vibrant red fluorescence. This capability empowers researchers to evaluate cell viability and cytotoxicity across diverse experimental settings with unparalleled precision.

Apoptosis Detection: In the realm of apoptosis studies, this compound plays a pivotal role in identifying late-stage apoptotic cells marked by compromised membranes that facilitate Ethidium homodimer-1 entry. When combined with complementary staining techniques, it delineates necrotic, apoptotic, and viable cells with exceptional clarity. This renders it an indispensable asset in unraveling cell death mechanisms and gauging the efficacy of pro-apoptotic interventions.

DNA Visualization: Within the realm of gel electrophoresis, Ethidium homodimer-1 emerges as a go-to option for DNA visualization, offering a compelling alternative to the prevalent ethidium bromide. Through binding to DNA fragments and fluorescing under ultraviolet light, it enables the visualization of DNA bands within agarose gels. Researchers leverage this characteristic to affirm the presence and size of nucleic acids in molecular cloning and PCR experiments, forging new pathways in genetic research.

Cytogenetics: In the domain of cytogenetic studies, Ethidium homodimer-1 assumes a critical role in chromosomal staining for subsequent fluorescent microscopy analysis. By binding to DNA and accentuating chromosomal regions, it aids in the identification of chromosomal structures and anomalies, crucial for karyotyping and diagnosing genetic disorders stemming from chromosomal aberrations. This application stands as a cornerstone in advancing our understanding of genetic intricacies and aberrations within the chromosomal landscape.