Biotin-11-UTP lithium salt

Biotin-11-UTP lithium salt is a biotinylated uridine triphosphate analog designed for enzymatic incorporation of biotin into RNA during in vitro transcription. The extended biotin linker provides efficient tag accessibility for downstream streptavidin-based capture and fluorescence labeling, enabling sensitive visualization and affinity enrichment of RNA populations in molecular biology workflows.

1. RNA In Vitro Transcription

Biotin-11-UTP lithium salt is used by molecular biology and chemical biology labs to generate biotin-labeled RNA transcripts for downstream pull-down workflows. Researchers incorporate the reagent into RNA during in vitro transcription to produce RNA probes, RNA standards, and affinity-tagged RNA materials that can be enriched using streptavidin-coated beads or membranes, supporting workflows such as RNA capture, purification, and sample normalization for downstream assays.

2. Streptavidin-Based RNA Detection

Biotin-11-UTP lithium salt supports fluorescence-based and chemiluminescent detection of RNA by enabling streptavidin conjugate binding to the biotin handles. Teams developing RNA detection assays use biotinylated transcripts as hybridization probes or as labeled RNA controls, then visualize or quantify signal after incubating with streptavidin-fluorophore conjugates, facilitating readouts in gel-based analyses, blotting workflows, and plate-based imaging platforms where biotin-streptavidin chemistry provides robust signal anchoring.

3. RNA Hybridization Probe Construction

Biotin-11-UTP lithium salt is frequently incorporated into RNA probe design for nucleic acid analysis workflows that rely on affinity-tagged hybridization products. Researchers generate biotin-containing antisense or RNA-like probes and use them in hybridization experiments where captured probe-target complexes are recovered using streptavidin surfaces, improving workflow practicality for separating bound from unbound fractions prior to detection by fluorescence or other streptavidin-dependent readouts.

4. RNA Pull-Down and Enrichment

Biotin-11-UTP lithium salt is applied in RNA interactome and RNA-processing studies where affinity enrichment of biotinylated RNA is required. By producing biotin-tagged RNA substrates or labeled RNAs, researchers can isolate RNA species from complex mixtures using streptavidin beads, enabling downstream analyses such as co-purified partner characterization, fractionation of RNA populations, and preparation of enriched RNA for downstream molecular assays.