Biotin-11-dUTP

Biotin-11-dUTP, a modified nucleotide widely utilized in molecular biology and biochemical assays, serves as a versatile tool in various applications. Here are four key applications of Biotin-11-dUTP:

DNA Labeling: Biotin-11-dUTP plays a pivotal role in labeling DNA molecules by integrating during DNA synthesis. This labeled DNA can be effectively identified and quantified using diverse biotin-binding detection systems, such as streptavidin-conjugated enzymes or fluorophores. This application proves invaluable in techniques like in situ hybridization, Southern blotting, and DNA sequencing, offering a nuanced approach to analyzing genetic material.

PCR Amplification: Within polymerase chain reaction (PCR) assays, Biotin-11-dUTP can be seamlessly incorporated into the amplified DNA fragments. This modification facilitates the straightforward and precise capture of PCR products utilizing streptavidin-coated surfaces, streamlining downstream analyses. Particularly beneficial in applications like DNA microarray analysis and diagnostic testing, this method underscores the adaptability of Biotin-11-dUTP in diverse scientific endeavors.

Protein-DNA Interactions: Leveraging Biotin-11-dUTP, researchers can craft biotin-labeled probes to investigate protein-DNA interactions comprehensively. These probes form intricate bonds with specific DNA sequences, and through streptavidin-mediated detection, researchers can dissect the binding characteristics and affinities of DNA-binding proteins. This approach stands as a fundamental tool in unraveling transcriptional regulation and delving into the dynamic world of chromatin, shedding light on the complexities of genetic expression.

Cell Imaging: By incorporating Biotin-11-dUTP into cells, researchers gain a visual gateway into DNA synthesis during cellular proliferation. Through the use of fluorescently labeled streptavidin, biotinylated DNA becomes readily detectable and can be imaged, offering insights into cell cycle dynamics and replication patterns.