Calcein AM | 148504-34-1

Calcein AM, a prominent fluorescent dye, finds extensive utility in evaluating cell viability and function. Here are four key applications of Calcein AM:

Cell Viability Assays: A standard practice in cell viability testing, Calcein AM serves to differentiate between live and deceased cells. Upon intracellular presence in live cells, Calcein AM undergoes hydrolysis by esterases, yielding a fluorescent compound – Calcein, emitting a vibrant green signal. This method, characterized by remarkable sensitivity, offers both visual and quantitative insights into cellular well-being.

Cell Tracking: Calcein AM emerges as a pivotal tool for tracking cells in research studies, allowing for the observation of cell migration and behavior, both in controlled environments and in vivo settings. The dye’s robust and enduring fluorescence properties permit prolonged tracking durations sans substantial signal loss. This application proves vital in investigations concerning cellular movement, tissue regeneration, and intercellular dynamics.

High-Throughput Screening: Distinguished for its adaptability with assorted plate readers and automated systems, Calcein AM stands out as the go-to choice for high-throughput screening analyses. This dye accelerates the evaluation of cell viability in extensive drug screening endeavors and assessments of compound efficacy. Consequently, it expedites the identification and advancement of novel therapeutic agents, propelling drug discovery efforts.

Calcium Signaling Studies: Delving into calcium signaling pathways within cells, Calcein AM serves as a compelling tool for researchers. Post-cellular penetration, the dye can sequester calcium ions, facilitating the measurement of intracellular calcium levels through modalities like fluorescence microscopy or flow cytometry. This application plays a pivotal role in unraveling cellular signaling cascades and physiological responses, shedding light on intricate molecular mechanisms within cells.