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(Z-Ala-Ala-Ala-Ala)2Rh110 | 149695-85-2
| Catalog Number | A18-0017 |
| Category | Fluorescent Enzyme Substrates |
| Molecular Formula | C60H66N10O15 |
| Molecular Weight | 1167.2 |
| Catalog Number | Size | Price | Quantity |
|---|---|---|---|
| A18-0017 | -- | $-- |
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Product Introduction
(Z-Ala-Ala-Ala-Ala)2Rh110 is a fluorogenic substrate for elastases. The colorless and nonfluorescent (Z-Ala-Ala-Ala-Ala)2Rh110 is cleaved by elastase to yield the highly fluorescent moiety rhodamine 110.
Chemical Information |
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| Synonyms | (Cbz-ala4)2-rhodamine; Bzt-ala-R; (Z-Ala-Ala-Ala-Ala)2Rhodamine110; bis-(CBZ-L-alanyl-L-arginine amide) Rhodamine 110; N-[(phenylmethoxy)carbonyl]-L-alanyl-L-alanyl-L-alanyl-N-[3-oxo-6'-[[N-[N-[N-[N-[(phenylmethoxy)carbonyl]-L-alanyl]-L-alanyl]-L-alanyl]-L-alanyl]amino]spiro[isobenzofuran-1(3H),9'-[9H]xanthen]-3'-yl]-L-alaninamide |
| Purity | ≥95% |
| Canonical SMILES | CC(C(=O)NC(=O)C(C)NC(=O)C(C)NC(=O)C(C)NC(=O)OCC1=CC=CC=C1)NC2=CC3=C(C=C2)C4(C5=C(O3)C=C(C=C5)NC(=O)C(C)NC(=O)C(C)NC(=O)C(C)NC(=O)C(C)NC(=O)OCC6=CC=CC=C6)C7=CC=CC=C7C(=O)O4 |
| InChI | InChI=1S/C60H66N10O15/c1-31(61-49(71)33(3)63-53(75)37(7)67-58(80)82-29-39-17-11-9-12-18-39)51(73)65-35(5)55(77)69-41-23-25-45-47(27-41)84-48-28-42(24-26-46(48)60(45)44-22-16-15-21-43(44)57(79)85-60)70-56(78)36(6)66-52(74)32(2)62-50(72)34(4)64-54(76)38(8)68-59(81)83-30-40-19-13-10-14-20-40/h9-28,31-38H,29-30H2,1-8H3,(H,61,71)(H,62,72)(H,63,75)(H,64,76)(H,65,73)(H,66,74)(H,67,80)(H,68,81)(H,69,77)(H,70,78)/t31-,32-,33-,34-,35-,36-,37-,38-/m0/s1 |
| InChI Key | JGBISHWUFSPAPR-QSVFAHTRSA-N |
| Appearance | Solid Powder |
- Product Specification
- Application
| Excitation | 485 nm |
| Emission | 525 nm |
| Storage | Store at -20°C |
(Z-Ala-Ala-Ala-Ala)2Rh110 is a specialized fluorogenic substrate commonly utilized in biochemical assays to measure elastase activity. Elastases are enzymes that break down proteins, and studying their function is critical in understanding various biological processes and diseases. (Z-Ala-Ala-Ala-Ala)2Rh110 itself is a nonfluorescent compound. However, when elastases cleave it, they release a highly fluorescent product, rhodamine 110. This transformation from a nonfluorescent to a fluorescent state makes (Z-Ala-Ala-Ala-Ala)2Rh110 an invaluable tool for observing and measuring enzyme activity in real time. Its high specificity for elastase and clear signal output enhance the precision of these measurements compared to other methods.
One of the primary applications of (Z-Ala-Ala-Ala-Ala)2Rh110 is in the evaluation of elastase inhibitors. In pharmaceutical research, identifying potent inhibitors is essential for developing new treatments for diseases such as emphysema and cystic fibrosis, where excessive enzyme activity is a problem. By mixing potential inhibitor compounds with (Z-Ala-Ala-Ala-Ala)2Rh110 and elastases, researchers can observe changes in fluorescence. A decrease in fluorescence indicates successful inhibition of elastase activity by the candidate drug, thus showcasing its potential therapeutic value.
Another crucial application is the use of (Z-Ala-Ala-Ala-Ala)2Rh110 in diagnostic assays for various inflammatory diseases. Elastase levels are often elevated in inflammatory conditions, and precise measurement is necessary for accurate diagnosis and monitoring. Its use in assays allows for rapid, sensitive detection of elastase activity in biological samples, providing clinicians with valuable information about the presence and progression of inflammation, thereby aiding in timely treatment planning and disease management.
Additionally, (Z-Ala-Ala-Ala-Ala)2Rh110 serves as a significant research tool in studying the pathophysiology of pulmonary diseases. By quantifying elastase activity in lung tissues, researchers can better understand the role these enzymes play in disease mechanisms, potentially leading to novel therapeutic strategies. Furthermore, this substrate is vital in environmental and industrial biotechnology, where elastase activity assays are used in processes like waste degradation and food processing. Its capacity to provide clear and immediate results makes it an indispensable resource across multiple research and industrial landscapes.
Applications of Fluorescent Probes & Dyes
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