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4-Methylumbelliferyl-β-D-Glucopyranoside | CAS 18997-57-4
| Catalog Number | A18-0103 |
| Category | Fluorescent Enzyme Substrates |
| Molecular Formula | C16H18O8 |
| Molecular Weight | 338.31 |
* Please be kindly noted products are not for therapeutic use. We do not sell to patients.
Product Introduction
4-Methylumbelliferyl-β-D-Glucopyranoside (4-MUG) is a fluorogenic substrate for β-glucosidase and β-glucocerebrosidase. 4-MUG is utilized to detect β-glucosidases as an indicator of Enterococci, and is also used in evaluating β- glucocerebrosidase activity related to Gaucher disease.
Chemical Information
Product Specification
Application
| Synonyms | 4-MU-GLU; 4-MU-β-Gluc; 4-MUG; MU-GLU; 7-(β-D-glucopyranosyloxy)-4-methyl-2H-1-benzopyran-2-one |
| Purity | ≥98% |
| IUPAC Name | 4-methyl-7-[(2S,3R,4S,5S,6R)-3,4,5-trihydroxy-6-(hydroxymethyl)oxan-2-yl]oxychromen-2-one |
| Canonical SMILES | CC1=CC(=O)OC2=C1C=CC(=C2)OC3C(C(C(C(O3)CO)O)O)O |
| InChI | InChI=1S/C16H18O8/c1-7-4-12(18)23-10-5-8(2-3-9(7)10)22-16-15(21)14(20)13(19)11(6-17)24-16/h2-5,11,13-17,19-21H,6H2,1H3/t11-,13-,14+,15-,16-/m1/s1 |
| InChIKey | YUDPTGPSBJVHCN-YMILTQATSA-N |
| Appearance | White to Pale Yellow Solid |
| Excitation | 330 nm (pH 4.6), 370 nm (pH 7.4), 385 nm (pH 10.4) |
| Emission | 445-454 nm |
| Storage | Store at -20°C |
4-Methylumbelliferyl-β-D-Glucopyranoside, a fluorogenic substrate, is utilized in diverse biochemical assays and research endeavors.
Enzyme Activity Assays: A cornerstone in the realm of enzymology, 4-Methylumbelliferyl-β-D-Glucopyranoside acts as a potent tool for assessing the activity of β-glucosidase enzymes. Upon enzymatic cleavage, this substrate liberates 4-methylumbelliferone, a fluorescent byproduct that facilitates the quantification of enzyme activity through fluorescence detection. This method finds widespread utility in both research settings and diagnostic laboratories, enabling in-depth investigations of enzyme kinetics and the screening for potential inhibitors.
Microbial Identification: In the intricate world of microbiology, 4-Methylumbelliferyl-β-D-Glucopyranoside emerges as a valuable resource for the identification and differentiation of microbial species harboring β-glucosidase activity. By supplementing microbial cultures with this substrate, scientists can discern fluorescence, a telltale sign of β-glucosidase-positive microbes. This technique proves instrumental in clinical diagnostics and environmental surveillance, offering swift and precise identification of pathogenic and industrially significant microorganisms.
Biomarker Discovery: Within the domain of biomedical exploration, 4-Methylumbelliferyl-β-D-Glucopyranoside serves as a linchpin for uncovering and validating biomarkers associated with metabolic irregularities and diseases. Utilizing this substrate, researchers scrutinize β-glucosidase activity in biological specimens, pinpointing aberrant enzyme functioning as a plausible biomarker. This application plays a pivotal role in elucidating disease mechanisms and tailoring diagnostic instruments for enhanced medical understanding.
Cellular Imaging: Harnessing the luminous attributes of 4-Methylumbelliferyl-β-D-Glucopyranoside, researchers embark on transformative live-cell imaging investigations. Employing this substrate, they peer into the realm of β-glucosidase activity within cells, unveiling nuances of enzyme distribution, activity patterns, and cellular metabolic workings. This visionary application empowers cell biology research, facilitating a deeper exploration of cellular functionality and disease intricacies.
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