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4-Methylumbelliferyl-β-D-Glucopyranoside | CAS 18997-57-4
| Catalog Number | A18-0103 |
| Category | Fluorescent Enzyme Substrates |
| Molecular Formula | C16H18O8 |
| Molecular Weight | 338.31 |
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Product Introduction
A widely used fluorogenic substrate for β-glucosidase enzyme activity. Ideal for fluorescence-based kinetic assays. 4-Methylumbelliferyl-β-D-Glucopyranoside is a synthetic glucoside substrate widely utilized in enzymatic assays for the detection of β-glucosidase activity. Featuring a stable, crystalline powder form, it releases a fluorescent 4-methylumbelliferone moiety upon enzymatic hydrolysis, enabling sensitive quantification. Its high specificity makes it valuable in glycosidase research, carbohydrate metabolism studies, and environmental monitoring. Researchers favor this compound for its robust performance in fluorescence-based analytical techniques.
Chemical Information
Product Specification
Application
| Synonyms | 4-MU-GLU; 4-MU-β-Gluc; 4-MUG; MU-GLU; 7-(β-D-glucopyranosyloxy)-4-methyl-2H-1-benzopyran-2-one |
| Purity | ≥98% |
| IUPAC Name | 4-methyl-7-[(2S,3R,4S,5S,6R)-3,4,5-trihydroxy-6-(hydroxymethyl)oxan-2-yl]oxychromen-2-one |
| Canonical SMILES | CC1=CC(=O)OC2=C1C=CC(=C2)OC3C(C(C(C(O3)CO)O)O)O |
| InChI | InChI=1S/C16H18O8/c1-7-4-12(18)23-10-5-8(2-3-9(7)10)22-16-15(21)14(20)13(19)11(6-17)24-16/h2-5,11,13-17,19-21H,6H2,1H3/t11-,13-,14+,15-,16-/m1/s1 |
| InChIKey | YUDPTGPSBJVHCN-YMILTQATSA-N |
| Appearance | White to Pale Yellow Solid |
| Melting Point | >195°C (dec.) |
| Excitation | 330 nm (pH 4.6), 370 nm (pH 7.4), 385 nm (pH 10.4) |
| Emission | 445-454 nm |
| Storage | Store at -20°C |
4-Methylumbelliferyl-β-D-Glucopyranoside, a fluorogenic substrate, is utilized in diverse biochemical assays and research endeavors.
Enzyme Activity Assays: A cornerstone in the realm of enzymology, 4-Methylumbelliferyl-β-D-Glucopyranoside acts as a potent tool for assessing the activity of β-glucosidase enzymes. Upon enzymatic cleavage, this substrate liberates 4-methylumbelliferone, a fluorescent byproduct that facilitates the quantification of enzyme activity through fluorescence detection. This method finds widespread utility in both research settings and diagnostic laboratories, enabling in-depth investigations of enzyme kinetics and the screening for potential inhibitors.
Microbial Identification: In the intricate world of microbiology, 4-Methylumbelliferyl-β-D-Glucopyranoside emerges as a valuable resource for the identification and differentiation of microbial species harboring β-glucosidase activity. By supplementing microbial cultures with this substrate, scientists can discern fluorescence, a telltale sign of β-glucosidase-positive microbes. This technique proves instrumental in clinical diagnostics and environmental surveillance, offering swift and precise identification of pathogenic and industrially significant microorganisms.
Biomarker Discovery: Within the domain of biomedical exploration, 4-Methylumbelliferyl-β-D-Glucopyranoside serves as a linchpin for uncovering and validating biomarkers associated with metabolic irregularities and diseases. Utilizing this substrate, researchers scrutinize β-glucosidase activity in biological specimens, pinpointing aberrant enzyme functioning as a plausible biomarker. This application plays a pivotal role in elucidating disease mechanisms and tailoring diagnostic instruments for enhanced medical understanding.
Cellular Imaging: Harnessing the luminous attributes of 4-Methylumbelliferyl-β-D-Glucopyranoside, researchers embark on transformative live-cell imaging investigations. Employing this substrate, they peer into the realm of β-glucosidase activity within cells, unveiling nuances of enzyme distribution, activity patterns, and cellular metabolic workings. This visionary application empowers cell biology research, facilitating a deeper exploration of cellular functionality and disease intricacies.
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