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Rhodamine B | CAS 81-88-9
| Catalog Number | A01-0005 |
| Category | Fluorescent Proteins & Nucleotides |
| Molecular Formula | C28H31ClN2O3 |
| Molecular Weight | 479.01 |
* Please be kindly noted products are not for therapeutic use. We do not sell to patients.
Product Introduction
Rhodamine B is a tracer dye within water to determine the rate and direction of flow and transport. Rhodamine dyes fluoresce and thus can be detected easily with instruments called fluorometers. Rhodamine dyes are used extensively in biotechnology applications such as fluorescence microscopy, flow cytometry, fluorescence correlation spectroscopy and ELISA.
Chemical Information
Product Specification
Application
Computed Properties
Patents
| Related CAS | 64381-98-2 (free base) 1118426-88-2 (Alternate CAS) |
| Synonyms | Xanthylium, 9-(2-carboxyphenyl)-3,6-bis(diethylamino)-, chloride (1:1); [9-(o-Carboxyphenyl)-6-(diethylamino)-3H-xanthen-3-ylidene]diethylammonium chloride; Ammonium, [9-(o-carboxyphenyl)-6-(diethylamino)-3H-xanthen-3-ylidene]diethyl-, chloride; 11411 Red; ADC Rhodamine B; Aizen Rhodamine B; Aizen Rhodamine BH; Aizen Rhodamine BHC; Akiriku Rhodamine B; Amezin Rhodamine B Liquid; Basazol Red 71L; Basazol Red 71P; Basic Rose Extract; Basic Rose Red; Basic Violet 10; Basonyl Red 540; Basonyl Red 545; Basonyl Red 545FL; Brilliant Pink B; C.I. 45170; C.I. Basic Violet 10; C.I. Food Red 15; Calcozine Red BX; Calcozine Rhodamine BXP; Cerise Toner X 1127; Chromatint Rhodamine B; D and C Red No. 19; D&C Red 19; D&C Red No. 19; Diabasic Rhodamine B; Edicol Supra Rose B; Edicol Supra Rose BS; Eriosin Rhodamine B; FD And C Red No. 19; Flexo Red 540; GRM 980; Hexacol Rhodamine B Extra; Ikada Rhodamine B; Japan Red 213; Japan Red No. 213; LC 6100; Mitsui Rhodamine BX; OP 312; Red No. 213; RHB; Rheonine B; Rhodamine 610 chloride; Rhodamine B 500; Rhodamine B 500 hydrochloride |
| Purity | ≥98% |
| IUPAC Name | [9-(2-carboxyphenyl)-6-(diethylamino)xanthen-3-ylidene]-diethylazanium;chloride |
| Canonical SMILES | CCN(CC)C1=CC2=C(C=C1)C(=C3C=CC(=[N+](CC)CC)C=C3O2)C4=CC=CC=C4C(=O)O.[Cl-] |
| InChI | InChI=1S/C28H30N2O3.ClH/c1-5-29(6-2)19-13-15-23-25(17-19)33-26-18-20(30(7-3)8-4)14-16-24(26)27(23)21-11-9-10-12-22(21)28(31)32;/h9-18H,5-8H2,1-4H3;1H |
| InChIKey | PYWVYCXTNDRMGF-UHFFFAOYSA-N |
| Solubility | Soluble in DMSO (Slightly), Methanol (Slightly) |
| Appearance | Very Dark Green to Black Solid |
| MDL Number | MFCD00011931 |
| LogP | 2.56500 |
| Maximum Absorption Wavelength | 554(H2O) nm |
| Storage | Store at -20°C under inert atmosphere |
| Signal | Danger |
| GHSHazardStatements | H302 (11.84%): Harmful if swallowed [Warning Acute toxicity, oral] H318 (99.09%): Causes serious eye damage [Danger Serious eye damage/eye irritation] H412 (94.72%): Harmful to aquatic life with long lasting effects [Hazardous to the aquatic environment, long-term hazard] |
| Precautionary Statement Codes | P264, P264+P265, P270, P273, P280, P301+P317, P305+P354+P338, P317, P330, and P501 (The corresponding statement to each P-code can be found at the GHS Classification page.) |
Rhodamine B, a versatile fluorescent dye widely utilized in diverse scientific disciplines, boasts an array of applications. Here are four key applications of Rhodamine B:
Biological Staining: A cornerstone in histology and cell biology, Rhodamine B stands as a beacon for staining and illuminating biological tissues and cellular entities. Its vibrant fluorescence illuminates cellular landscapes under microscopic scrutiny, offering researchers a clear view of intricate structures. Particularly adept at labeling mitochondria, cytoskeleton components, and various intracellular organelles, Rhodamine B facilitates in-depth imaging studies.
Fluorescence Microscopy: Powering the realm of fluorescence microscopy, Rhodamine B emerges as a paramount fluorophore for tracing biomolecules and capturing dynamic cellular processes in action. Its robust emission characteristics render it a prime candidate for live-cell imaging, unveiling real-time cellular dynamics. Researchers harness its prowess to monitor protein localization and motility, as well as to track cellular signaling cascades, shedding light on the intricacies of cellular behavior.
Flow Cytometry: Harnessing the brilliance of Rhodamine B, flow cytometry leaps toward new frontiers in the analysis and sorting of cells based on their fluorescent signatures. By tagging distinct cell populations with Rhodamine B-conjugated antibodies or staining techniques, scientists discern between cell types and quantify cellular attributes. This technique proves paramount for endeavors such as immunophenotyping, apoptosis studies, and cell cycle analysis, empowering researchers to delve deeper into cellular functionalities.
Chemical Sensing: Spearheading the domain of chemical sensing, Rhodamine B plays a pivotal role in crafting sensors and probes for detecting a myriad of analytes. Its fluorescence undergoes metamorphosis in response to environmental cues, rendering it a sensitive indicator for pH levels, ions, and assorted substances. These sensors find applications across domains like environmental surveillance, biomedical diagnostics, and industrial processes, showcasing the versatility and precision of Rhodamine B in the realm of chemical detection.
| Hydrogen Bond Donor Count | 1 |
| Hydrogen Bond Acceptor Count | 5 |
| Rotatable Bond Count | 7 |
| Exact Mass | 478.2023205 g/mol |
| Monoisotopic Mass | 478.2023205 g/mol |
| Topological Polar Surface Area | 52.8Ų |
| Heavy Atom Count | 34 |
| Formal Charge | 0 |
| Complexity | 811 |
| Isotope Atom Count | 0 |
| Defined Atom Stereocenter Count | 0 |
| Undefined Atom Stereocenter Count | 0 |
| Defined Bond Stereocenter Count | 0 |
| Undefined Bond Stereocenter Count | 0 |
| Covalently-Bonded Unit Count | 2 |
| Compound Is Canonicalized | Yes |
| Publication Number | Title | Priority Date |
|---|---|---|
| US-11529315-B1 | Modified guanidine-containing polymers for biologic delivery | 2022-01-19 |
| WO-2022078524-A2 | Specific conjugation of an antibody | 2021-11-03 |
| WO-2022078524-A4 | Specific conjugation of an antibody | 2021-11-03 |
| US-11535900-B1 | Methods and systems for analysis of samples containing particles used for gene delivery | 2021-09-03 |
| US-2022411856-A1 | Compact optical high-speed system for nucleic acid amplification and detection | 2021-06-29 |
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