Texas Red-X | CAS 199745-67-0

Texas Red-X is a red-emitting, xanthene-based fluorescent dye building block commonly used for fluorescent labeling workflows where a reactive handle is needed to attach the fluorophore to biomolecules or materials. In typical research use, Texas Red-X is incorporated into conjugates for fluorescence microscopy and fluorescence-based assays, leveraging its strong visible emission and convenient dye chemistry for downstream probe and reagent construction.

1. Biomolecule Fluorescent Labeling

Texas Red-X is frequently used by chemical biology and protein chemistry groups to generate fluorescently labeled biomolecules for visualization and quantification. Researchers conjugate Texas Red-X to peptides, proteins, antibodies, and other labeling targets to track binding, localization, and transport in assay formats that read out red fluorescence. The dye's bright, red spectral profile is especially useful when multiplexing with green or far-red channels in microscopy and plate-based fluorescence measurements.

2. Fluorescence Microscopy Staining

Texas Red-X supports fluorescence microscopy staining and imaging reagent development for fixed-sample workflows where red fluorescence provides clear contrast. Imaging teams use Texas Red-X conjugates to label extracellular components, biomolecular targets, or engineered constructs on cells and biomaterial surfaces, enabling spatial mapping of where labeled species accumulate. Its xanthene emission is also commonly leveraged for multi-color imaging strategies that require separation from common green fluorophores.

3. Flow Cytometry Fluorescent Conjugates

Texas Red-X is used to prepare red fluorescent conjugates for flow cytometry experiments that require robust signal readout in the visible range. Cytometry users generate labeled antibodies and affinity reagents with Texas Red-X to quantify binding to cell-surface markers or to monitor labeling intensity in population-based studies. This application is particularly common in assay development and method optimization where consistent red-channel fluorescence supports gating and comparative analysis across experimental conditions.

4. Fluorescent Bioassay Reporter Construction

Texas Red-X is incorporated into fluorescent reporter reagents for fluorescence-based bioassays and binding studies that quantify signal changes through red emission. Assay developers use Texas Red-X labeling to create conjugate-based detection reagents for plate reader measurements, including formats built around fluorescent tagging of assay components. The dye's compatibility with standard fluorescence instrumentation and its strong visible emission make it a practical choice for constructing assay tools where red fluorescence provides a reliable readout.