By-3 Pro Phosphoramidite

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By-3 Pro Phosphoramidite

By-3 Pro Phosphoramidite

Catalog Number R10-0031
Category Phosphoramidites
Molecular Formula C65H81BF4N5O6P
Molecular Weight 1146.17
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Product Introduction

By-3 Pro Phosphoramidite is a fluorescent labeling reagent used in oligonucleotide synthesis. It contains a By-3 fluorophore attached to a hydroxyprolinol-based phosphoramidite moiety. The hydroxyprolinol linker provides a rigid, non-nucleosidic scaffold for efficient incorporation of the By-3 label at the 5' end of DNA or RNA sequences during automated solid-phase synthesis. It is commonly used in multiplex PCR, DNA sequencing, and fragment analysis applications.

  • Chemical Information

Chemical Information

Synonyms Cyanine3 Pro Phosphoramidite tetrafluoroborate; Cyanine3 Phosphoramidite (hydroxyprolinol) tetrafluoroborate; Cy3 Pro Phosphoramidite tetrafluoroborate; 2-((E)-3-((E)-1-(6-((2S,4R)-2-((Bis(4-methoxyphenyl)(phenyl)methoxy)methyl)-4-(((2-cyanoethoxy)(diisopropylamino)phosphaneyl)oxy)pyrrolidin-1-yl)-6-oxohexyl)-3,3-dimethylindolin-2-ylidene)prop-1-en-1-yl)-1,3,3-trimethyl-3H-indol-1-ium tetrafluoroborate
SMILES COC1=CC=C(C(C2=CC=CC=C2)(OC[C@@H]3C[C@H](CN3C(CCCCCN4C5=C(C(C)(/C4=C\C=C\C6=[N+](C7=C(C(C)6C)C=CC=C7)C)C)C=CC=C5)=O)OP(OCCC#N)N(C(C)C)C(C)C)C(C=C8)=CC=C8OC)C=C1
InChI InChI=1S/C65H81N5O6P/c1-47(2)70(48(3)4)77(75-43-23-41-66)76-55-44-52(46-74-65(49-24-14-12-15-25-49,50-33-37-53(72-10)38-34-50)51-35-39-54(73-11)40-36-51)69(45-55)62(71)32-16-13-21-42-68-59-29-20-18-27-57(59)64(7,8)61(68)31-22-30-60-63(5,6)56-26-17-19-28-58(56)67(60)9/h12,14-15,17-20,22,24-31,33-40,47-48,52,55H,13,16,21,23,32,42-46H2,1-11H3/q+1/t52-,55+,77?/m0/s1
InChIKey DUSPVBGYZWCGOL-LHVZTRALSA-N

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