Quasar 570 CE Phosphoramidite | CAS 1032678-27-5

Quasar 570 CE Phosphoramidite is a fluorescent dye phosphoramidite designed for incorporation into nucleic acids via standard solid-phase DNA or RNA synthesis workflows. As a click-chemistry-adjacent labeling reagent used in probe and oligonucleotide construction, it enables post-synthetic conjugation strategies when combined with complementary functional handles and typical bioconjugation pipelines. The dye’s hydrophobic, high-brightness fluorophore core and phosphoramidite compatibility make it a practical choice for building imaging-capable oligonucleotide reagents used in molecular diagnostics research, assay development, and molecular imaging toolkits.

1. Fluorescent Oligonucleotide Probes

Quasar 570 CE Phosphoramidite is commonly used to generate sequence-defined fluorescent oligonucleotide probes for hybridization-based detection formats, including nucleic acid sensing and target-binding assays in research laboratories. Incorporating the dye directly during oligonucleotide synthesis provides a robust way to control labeling position and stoichiometry, which is important for optimizing signal-to-background in fluorescence readouts. Researchers also use Quasar 570 CE Phosphoramidite when designing probe sets for multiplex workflows where spectral separation and consistent labeling density across constructs are critical for downstream imaging and quantification.

2. Molecular Imaging and Tracking

Quasar 570 CE Phosphoramidite supports the construction of fluorescent nucleic acid imaging reagents used for tracking biomolecular interactions and studying nucleic-acid dynamics in chemical biology and biomaterials research. By embedding the fluorophore into oligonucleotide scaffolds, investigators can create probes that report on binding events, localization, and temporal changes in experimental systems that rely on fluorescence microscopy or plate-based fluorescence. Quasar 570 CE Phosphoramidite is frequently selected for imaging tool development because it integrates seamlessly into automated synthesis and yields labeled oligos suitable for coupling with additional functional moieties in multi-component experimental designs.

3. Bioconjugation Handle Integration

Quasar 570 CE Phosphoramidite is often incorporated into oligonucleotides intended for further bioconjugation, including workflows where the fluorescent label must be positioned alongside an orthogonal reactive handle for subsequent attachment to biomolecules or surfaces. In these downstream development pipelines, the phosphoramidite enables precise dye placement while leaving other sites available for conjugation strategies that support assembly of labeled constructs such as affinity reagents, immobilized detection elements, or multi-ligand probes. Quasar 570 CE Phosphoramidite therefore fits well into modular reagent design, where fluorescence reporting and conjugation functionality are engineered into the same oligonucleotide platform.

4. Diagnostic Assay Development

Quasar 570 CE Phosphoramidite is used in the development of fluorescence-based diagnostic research reagents, particularly where nucleic acid probes, primers, or hybridization components require stable, sequence-controlled dye labeling. Assay developers value the ability to synthesize labeled oligonucleotides with defined dye incorporation patterns, enabling reproducible assay performance across screening batches and enabling systematic optimization of probe length and labeling position. Quasar 570 CE Phosphoramidite also supports the creation of standardized reagent panels for assay prototyping, method comparison, and signal optimization in laboratory settings that use fluorescence instrumentation for readout.