By-3.5 Pro Phosphoramidite

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By-3.5 Pro Phosphoramidite

By-3.5 Pro Phosphoramidite

Catalog Number R10-0033
Category Phosphoramidites
Molecular Formula C76H91BF4N5O6P
Molecular Weight 1288.37
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Product Introduction

By-3.5 Pro Phosphoramidite is a fluorescent labeling reagent used in oligonucleotide synthesis. It contains a By-3.5 fluorophore attached to a hydroxyprolinol-based phosphoramidite moiety. The hydroxyprolinol linker provides a rigid, non-nucleosidic scaffold for efficient incorporation of the By-3.5 label at the 5' end of DNA or RNA sequences during automated solid-phase synthesis. It is commonly used in multiplex PCR, DNA sequencing, and fragment analysis applications.

  • Chemical Information

Chemical Information

Synonyms Cyanine3.5 Pro Phosphoramidite tetrafluoroborate; Cyanine3.5 Phosphoramidite (hydroxyprolinol) tetrafluoroborate; Cy3.5 Pro Phosphoramidite tetrafluoroborate; 2-((1E,3E)-3-(3-(6-((2S,4R)-2-((Bis(4-methoxyphenyl)(phenyl)methoxy)methyl)-4-(((2-cyanoethoxy)(diisopropylamino)phosphaneyl)oxy)pyrrolidin-1-yl)-6-oxohexyl)-1,1-dimethyl-1,3-dihydro-2H-benzo[e]indol-2-ylidene)prop-1-en-1-yl)-3-butyl-1,1-dimethyl-1H-benzo[e]indol-3-ium tetrafluoroborate
SMILES FB(F)F.COC1=CC=C(C(C2=CC=CC=C2)(OC[C@@H]3C[C@H](CN3C(CCCCCN4C5=C(C(C)(/C4=C\C=C\C6=[N+](C7=C(C(C)6C)C8=CC=CC=C8C=C7)CCCC)C)C9=CC=CC=C9C=C5)=O)OP(OCCC#N)N(C(C)C)C(C)C)C(C=C%10)=CC=C%10OC)C=C1.[F-]
InChI InChI=1S/C76H91N5O6P.BF3.FH/c1-12-13-48-78-67-45-35-56-26-19-21-30-65(56)72(67)74(6,7)69(78)32-24-33-70-75(8,9)73-66-31-22-20-27-57(66)36-46-68(73)79(70)49-23-15-18-34-71(82)80-52-64(87-88(86-50-25-47-77)81(54(2)3)55(4)5)51-61(80)53-85-76(58-28-16-14-17-29-58,59-37-41-62(83-10)42-38-59)60-39-43-63(84-11)44-40-60;2-1(3)4;/h14,16-17,19-22,24,26-33,35-46,54-55,61,64H,12-13,15,18,23,25,34,48-53H2,1-11H3;;1H/q+1;;/p-1/t61-,64+,88?;;/m0../s1
InChIKey MFTGQLFAOMHUQN-FTOOFCBGSA-M

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