BOC Sciences provides high-quality phosphoramidites to manufacturers of oligonucleotides (oroligoribonucleotides).
The phosphoramidite method is one of the strategies of nucleic acid chemical synthesis. Each cycle of the synthesis of nucleic acid chains is through high-efficiency and chemically active nucleotide 3'-phosphoramidite intermediates, which become stable pentavalent phosphate triesters after oxidation. The phosphoramidite method is currently the most commonly used DNA automated solid-phase synthesis method.
The phosphoramidite unit synthesized by DNA is mainly composed of bases, deoxyribose, 5'-DMT (4',4'-dimethoxytrityl) and 3'end 2-cyanoethyl-N, N, N ', N'-Tetraisopropyl phosphorodiamidite composition. In the phosphoramidite unit of RNA synthesis, the hydroxyl group at position 2 needs to be protected to prevent cross-reaction between 2'-hydroxyl and 3'-hydroxyl (or 5'-hydroxyl) during solid-phase synthesis. The protecting group is generally tert-butyldimethylsilyl (TBDMS). In addition, if there are primary amino groups outside the ring on adenine, cytosine, guanine and some other bases, the primary amino groups need to be protected with protecting groups.
The phosphoramidite method completes DNA synthesis on a solid-phase carrier, and the direction of synthesis is synthesis from the 3'end to the 5'end of the primer to be synthesized.