Solid Chemical phosphorylation reagent II | CAS 202284-84-2

Catalog Number	R10-0003
Category	Phosphoramidites
Molecular Formula	C37H49N4O7P
Molecular Weight	692.79

Catalog Number	Size	Price	Quantity
R10-0003	250 mg	$299

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Product Introduction

Solid Chemical phosphorylation reagent II is a synthetic building block used for preparation and functionalization of more complex molecular structures. Its value lies in the functional groups and scaffold present in the molecule, which can be used for stepwise coupling, protection/deprotection, or derivatization according to the target synthesis. The product should be selected according to the reaction planned for its available functional groups and the compatibility of the remaining scaffold with downstream chemistry. Reaction conditions, protecting-group strategy, solubility, and purification requirements should be optimized for the specific synthetic application.

Chemical Information
Product Specification
Application
QC Data

Chemical Information

Synonyms	Chemical phosphorylation amidite; [3-(4,4'-Dimethoxytrityloxy)-2,2-dicarboxymethylamido]propyl-(2-cyanoethyl)-(N,N-diisopropyl)-phosphoramidite; Phosphoramidous acid, bis(1-methylethyl)-, 2-[[bis(4-methoxyphenyl)phenylmethoxy]methyl]-3-(methylamino)-2-[(methylamino)carbonyl]-3-oxopropyl 2-cyanoethyl ester; Solid Chemical Phosphorylation Reagent 2; 2-((bis(4-methoxyphenyl)(phenyl)methoxy)methyl)-3-(methylamino)-2-(methylcarbamoyl)-3-oxopropyl(2-cyanoethyl)(N,N-diisopropyl)phosphoramidite
Purity	≥95%
IUPAC Name	2-[[bis(4-methoxyphenyl)-phenylmethoxy]methyl]-2-[[2-cyanoethoxy-[di(propan-2-yl)amino]phosphanyl]oxymethyl]-N,N'-dimethylpropanediamide
SMILES	CC(C)N(C(C)C)P(OCCC#N)OCC(COC(C1=CC=CC=C1)(C2=CC=C(C=C2)OC)C3=CC=C(C=C3)OC)(C(=O)NC)C(=O)NC
InChI	InChI=1S/C37H49N4O7P/c1-27(2)41(28(3)4)49(47-24-12-23-38)48-26-36(34(42)39-5,35(43)40-6)25-46-37(29-13-10-9-11-14-29,30-15-19-32(44-7)20-16-30)31-17-21-33(45-8)22-18-31/h9-11,13-22,27-28H,12,24-26H2,1-8H3,(H,39,42)(H,40,43)
InChIKey	HAUCJKGKHJPPNN-UHFFFAOYSA-N
Appearance	Off-white Solid

Product Specification

Storage

Store at -20°C

Application

Solid Chemical phosphorylation reagent II is a solid-supported phosphorylation reagent designed for efficient transfer of phosphate groups to target molecules under click-chemistry-adjacent, workflow-friendly conditions used in chemical biology and biomaterials research. As a reagent platform that streamlines phosphorylation handling and minimizes solution-phase manipulation, it is commonly selected when researchers need reproducible phosphorylation derivatization for nucleotides, peptides, and other functional substrates. Its solid format and reagent identity make it particularly relevant for downstream conjugation and labeling workflows where controlled installation of phosphate handles improves compatibility with analytical readouts and affinity-based assays.

1. Phosphopeptide Enrichment

Solid Chemical phosphorylation reagent II is used to generate phosphorylated peptide standards and phosphopeptide libraries for mass spectrometry and phosphoproteomics method development. Researchers in chemical biology and proteomics frequently rely on consistent phosphorylation installation to benchmark enrichment strategies, optimize digestion and cleanup steps, and validate phosphosite-specific workflows. The solid-supported format supports repeatable batch processing, which is valuable when preparing multiple substrate sets for comparative MS method evaluation and reagent screening.

2. Biomolecule Conjugation Handles

Solid Chemical phosphorylation reagent II supports the creation of phosphate-functional biomolecule derivatives used as modular handles for subsequent chemical coupling and surface attachment. In molecular imaging and diagnostic reagent development, phosphate groups are often incorporated to tune binding interactions, improve stability of labeling intermediates, or enable orthogonal attachment schemes in complex assay formats. Solid Chemical phosphorylation reagent II is therefore commonly selected in research pipelines that require phosphorylated building blocks for constructing labeled probes, reagent controls, and multi-component conjugates.

3. Surface Functionalization Chemistry

Solid Chemical phosphorylation reagent II is applied in biomaterials science to introduce phosphate functionality onto polymeric or material surfaces that will be used for affinity capture, controlled biomolecular immobilization, or structured surface chemistry. Materials teams often prefer solid-supported phosphorylation reagents when they need practical surface-to-solution workflows with manageable handling and consistent surface derivatization across multiple substrates. Phosphate installation can also be used to create standardized surface chemistry for studying protein adsorption behavior, immobilized receptor interactions, and assay platform reproducibility.

4. Nucleotide and Co-factor Labeling

Solid Chemical phosphorylation reagent II is used to prepare phosphorylated nucleotide analogs and phosphate-bearing cofactors used in biochemical assay development and chemical biology toolkits. Researchers preparing labeled or modified nucleotide reagents often require reliable phosphorylation incorporation to support downstream detection chemistries, binding assays, and substrate compatibility testing. The solid reagent format helps streamline preparation of phosphorylated nucleotide intermediates that are then carried into labeling, immobilization, or analytical method validation workflows.