5'-O-DMT-2'-deoxyuridine-3'-CE Phosphoramidite | CAS 109389-30-2

Catalog Number	R10-0013
Category	Phosphoramidites
Molecular Formula	C39H47N4O8P
Molecular Weight	730.80

Catalog Number	Size	Price	Quantity
R10-0013	10 g	$439

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Product Introduction

5'-O-DMT-2'-deoxyuridine-3'-CE Phosphoramidite stands as an indispensable entity within the realm of biomedicine, wherein its applications for the synthesis of nucleic acids in investigative capacities hold paramount significance. DNA and RNA oligonucleotides rely on this product as their cornerstone, as it seamlessly integrates 2'-deoxyuridine into nucleic acid sequences with exacting efficiency. Aiding the progression of pharmaceutical innovation, gene therapy, and genetic exploration, it emerges as an invaluable instrument that propels advancements in the remediation of diverse medical afflictions.

Chemical Information
Product Specification
Application

Chemical Information

Synonyms	5'-O-(4,4'-Dimethoxytrityl)-2'-deoxyuridine-3'-O-[O-(2-cyanoethyl)-N,N'-diisopropylphosphoramidite]; 2'-Deoxy-5'-O-DMT-uridine 3'-CE Phosphoramidite; DMT-dU Phosphoramidite; 5-O-(4,4'-dimethoxytrityl)-3-O-(diisopropylamino-2-cyanoethoxyphosphanyl)-1,2-dideoxy-1-uracil-1-yl-beta-D-erythro-pentofuranose; 3'-O-[(Diisopropylamino)(2-cyanoethoxy)phosphino]-5'-O-(4,4'-dimethoxytrityl)-2'-deoxyuridine
Purity	≥95%
IUPAC Name	3-[[(2R,3S,5R)-2-[[bis(4-methoxyphenyl)-phenylmethoxy]methyl]-5-(2,4-dioxopyrimidin-1-yl)oxolan-3-yl]oxy-[di(propan-2-yl)amino]phosphanyl]oxypropanenitrile
SMILES	CC(C)N(C(C)C)P(OCCC#N)OC1CC(OC1COC(C2=CC=CC=C2)(C3=CC=C(C=C3)OC)C4=CC=C(C=C4)OC)N5C=CC(=O)NC5=O
InChI	InChI=1S/C39H47N4O8P/c1-27(2)43(28(3)4)52(49-24-10-22-40)51-34-25-37(42-23-21-36(44)41-38(42)45)50-35(34)26-48-39(29-11-8-7-9-12-29,30-13-17-32(46-5)18-14-30)31-15-19-33(47-6)20-16-31/h7-9,11-21,23,27-28,34-35,37H,10,24-26H2,1-6H3,(H,41,44,45)/t34-,35+,37+,52?/m0/s1
InChIKey	ZGJUDTLSWZPIDW-MVFNBKNKSA-N
Appearance	White to off-white powder

Product Specification

Storage

Store at 2-8°C

Application

5'-O-DMT-2'-deoxyuridine-3'-CE Phosphoramidite is a DNA/RNA building-block phosphoramidite designed for automated solid-phase oligonucleotide synthesis, where the 2'-deoxyuridine base is protected as a DMT ether at the 5'-position and the 3'-position is functionalized with a CE handle for subsequent click-type conjugation workflows. As a click-compatible oligonucleotide reagent, it is commonly selected when researchers need to introduce a defined reactive moiety into nucleic acid constructs for downstream labeling, imaging, or surface immobilization. The phosphoramidite format enables precise incorporation into synthetic strands, supporting reproducible placement of the reactive handle for bioconjugation and molecular imaging tool development.

1. Click-Ready Oligonucleotide Labeling

5'-O-DMT-2'-deoxyuridine-3'-CE Phosphoramidite is used to generate click-functional nucleic acid probes that carry a defined conjugation site at a specific position within an oligonucleotide sequence. Researchers incorporate this building block during standard automated synthesis to obtain strands suitable for subsequent click-based attachment of fluorophores, affinity tags, or reporter groups. This approach is especially valuable when probe performance depends on consistent labeling density and positional control, such as for hybridization-based assays, binding studies, and platform development for nucleic-acid detection reagents.

2. Molecular Imaging and Probe Development

5'-O-DMT-2'-deoxyuridine-3'-CE Phosphoramidite supports the construction of imaging-oriented oligonucleotide reagents by enabling post-synthetic installation of imaging labels through click-compatible conjugation chemistry. Molecular imaging tool developers use sequence-defined probes to improve signal uniformity and to tune the distance between the nucleic-acid recognition element and the imaging reporter. The phosphoramidite’s integration into oligonucleotides helps maintain structural integrity while providing a handle for attaching dyes, luminescent reporters, or other visualization components used in research imaging workflows.

3. Surface Immobilized DNA Materials

5'-O-DMT-2'-deoxyuridine-3'-CE Phosphoramidite is applied in the preparation of DNA-functionalized surfaces and materials where spatially controlled attachment of oligonucleotides is required. By incorporating the click handle into the strand during synthesis, materials scientists can later conjugate the oligonucleotide to surfaces, linkers, or material coatings using click-based coupling strategies compatible with surface chemistry constraints. This enables fabrication of DNA-based capture layers, patterned probe arrays, and hybridization-ready interfaces for analytical and research instrumentation.

4. Targeted Bioconjugation Linkers

5'-O-DMT-2'-deoxyuridine-3'-CE Phosphoramidite is frequently selected for building nucleic-acid conjugates that require a reliable attachment point to biomolecules or macromolecular carriers. In chemical biology and biomaterials workflows, oligonucleotides containing the CE handle are used as modular scaffolds that can be clicked onto proteins, polymers, or other functional components after synthesis. Positional control afforded by phosphoramidite incorporation helps researchers design conjugates with predictable architecture, supporting reproducible assembly of nucleic-acid-based constructs used in mechanistic studies and reagent development.