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Cell membrane fluorescent probes are a class of lipophilic fluorescent dyes. They are used to label cell membranes and hydrophobic tissues. They are stored in -20°C in the dark and dry and dissolved in DMF, DMSO, and methanol. They have high quenching coefficient, polarization dependence and very short excitation lifetime.
Characteristics of cell membrane fluorescent probes
- Cell membrane fluorescent probes, as environment-sensitive fluorescent dyes, their fluorescence intensity is significantly enhanced when they are bound to membranes or to lipophilic biomolecules (such as proteins, although their fluorescence intensity is weak in water). Once they enter inside the cell, they gradually diffuse through the intracellular plasma membrane and, at the right concentration, stain the entire cell uniformly. The stock solution of cell membrane fluorescent probes can be prepared with ethanol and they do not affect cell viability. Paraformaldehyde (other reagents such as methanol cannot be used) can be fixed after cell membrane fluorescent probes staining, but permeabilization after staining is not recommended.
Application of cell membrane fluorescent probes
- Cell membrane fluorescent probes are to label cell membranes and are tracers or long-term tracers for cells or tissues such as nerves, either forward or reverse, live or fixed. Other hydrophobic structures, such as exosomes, can also be labeled.
- Cell membrane fluorescent probes are very useful in in vivo imaging or tracking, because the infrared light they emit can efficiently pass cells and tissues. And in the infrared light range, their background fluorescence level is very low.
- Cell membrane fluorescent probes can be used to detect cell fusion and adhesion, detect cell migration during development or transplantation, detect lipid diffusion on cell membranes by FRAP (Fluorescence Recovery After Photobleaching), detect cytotoxicity and label lipoproteins, etc.