Live Imaging of the Extracellular Matrix With a Glycan-Binding Fluorophore

June 9th, 2026 10:00 AM EDT

Email:

Home
Products
Services
Solutions
Resources
Company

✖

We use cookies to understand how you use our site and to improve the overall user experience. This includes personalizing content and advertising. Read our Privacy Policy

Home
Products
Fluorescent Probes
Cell and Organelle Stains
Cell membrane Fluorescent Probes
Bis-(1,3-diethylthiobarbituric acid)trimethineoxonol

What We Offer

Bis-(1,3-diethylthiobarbituric acid)trimethineoxonol

Bis-(1,3-diethylthiobarbituric acid)trimethineoxonol | CAS 47623-98-3

Catalog Number	A16-0161
Category	Cell membrane Fluorescent Probes
Molecular Formula	C19H24N4O4S2
Molecular Weight	436.54

Inquiry

* Please be kindly noted products are not for therapeutic use. We do not sell to patients.

Worldwide Delivery
Quality Assurance
24/7 Customer Service

Product Introduction

Bis-(1,3-diethylthiobarbituric acid)trimethineoxonol is a fluorescent dye characterized by its conjugated trimethine bridge and thiobarbituric acid moieties, which contribute to its distinctive chromophore class. This compound exhibits absorption and emission properties that are sensitive to environmental changes, making it suitable for use in membrane potential studies and cellular imaging applications. It is commonly utilized in fluorescence microscopy and flow cytometry as a membrane probe due to its ability to integrate into lipid bilayers and report on dynamic cellular processes.

Chemical Information
Product Specification
Application

Chemical Information

Synonyms	DiSBAC2(3)
SMILES	CCN1C(=O)C(C(=O)N(C1=S)CC)C=CC=C2C(=O)N(C(=S)N(C2=O)CC)CC
InChI	InChI=1S/C19H24N4O4S2/c1-5-20-14(24)12(15(25)21(6-2)18(20)28)10-9-11-13-16(26)22(7-3)19(29)23(8-4)17(13)27/h9-12H,5-8H2,1-4H3/b10-9+
InChIKey	VJYNRXFXHKIGLT-MDZDMXLPSA-N

Product Specification

Excitation	554
Emission	580

Application

Bis-(1,3-diethylthiobarbituric acid) trimethineoxonol, commonly known as DiSBAC2(3), is a membrane potential-sensitive dye with diverse applications in bioscience. Here are four key applications of Bis-(1,3-diethylthiobarbituric acid) trimethineoxonol:

Membrane Potential Studies: DiSBAC2(3) serves as a versatile tool for investigating changes in membrane potential across various cell types. By integrating into the lipid bilayer, the dye undergoes fluorescence alterations corresponding to membrane depolarization and hyperpolarization. This unique property enables researchers to track the electrical dynamics of cells in real-time, offering invaluable insights into neuronal activity and cardiac physiology.

Drug Screening: Employed in high-throughput screening assays, DiSBAC2(3) aids in the evaluation of compound effects on cellular excitability. Through the detection of shifts in membrane potential, researchers can pinpoint potential drug candidates that modulate ion channels or other membrane-associated proteins. This particular application holds significant promise in the realm of pharmaceutical development for neurological and cardiovascular conditions.

Toxicology Studies: DiSBAC2(3) emerges as a critical asset in assessing the impact of environmental toxins and pharmaceutical agents on cellular well-being. Changes in membrane potential serve as key indicators of cell viability and functionality under toxic stressors. This makes the dye a pivotal instrument in exploring cellular responses to harmful substances and ensuring the safety profile of novel chemical entities.

Microbial Physiology: Within the field of microbiology, DiSBAC2(3) finds utility in probing membrane potential dynamics within bacteria and other microorganisms. Through the application of this dye, researchers can investigate how various environmental factors or antimicrobial agents influence bacterial membrane potential. This knowledge is fundamental for deciphering microbial behaviors, understanding pathogenicity, and crafting innovative antimicrobial strategies to combat infectious diseases.