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Calcein Blue AM | CAS 168482-84-6
| Catalog Number | A16-0035 |
| Category | Cell membrane Fluorescent Probes |
| Molecular Formula | C21H23NO11 |
| Molecular Weight | 465.4 |
* Please be kindly noted products are not for therapeutic use. We do not sell to patients.
Product Introduction
Calcein Blue AM is an acetoxymethyl ester of Calcein Blue, a fluorogenic dye used to assess cell viability. Calcein Blue AM is hydrolyzed by intracellular esterases to release the fluorescent probe Calcein Blue. Calcein Blue is retained by living cells and displays excitation/emission maxima of 360/445 nm, respectively.
Chemical Information
Product Specification
Application
Computed Properties
| Synonyms | Calcein Blue Acetoxymethyl ester; N-[2-[(acetyloxy)methoxy]-2-oxoethyl]-N-[(7-hydroxy-4-methyl-2-oxo-2H-1-benzopyran-8-yl)methyl]-glycine, (acetyloxy)methyl ester |
| Purity | ≥90% |
| IUPAC Name | acetyloxymethyl 2-[[2-(acetyloxymethoxy)-2-oxoethyl]-[(7-hydroxy-4-methyl-2-oxochromen-8-yl)methyl]amino]acetate |
| Canonical SMILES | CC1=CC(=O)OC2=C1C=CC(=C2CN(CC(=O)OCOC(=O)C)CC(=O)OCOC(=O)C)O |
| InChI | InChI=1S/C21H23NO11/c1-12-6-18(26)33-21-15(12)4-5-17(25)16(21)7-22(8-19(27)31-10-29-13(2)23)9-20(28)32-11-30-14(3)24/h4-6,25H,7-11H2,1-3H3 |
| InChIKey | GSFJGCPBBHLSPH-UHFFFAOYSA-N |
| Appearance | Solid Powder |
| Excitation | 360 nm |
| Emission | 445 nm |
| Storage | Store at -20°C |
Calcein Blue AM, a fluorescent dye widely utilized in molecular biology and biochemistry, boasts a myriad of applications. Here are four key applications of Calcein Blue AM:
Cell Viability Assays: In the dynamic realm of cell viability assessments, Calcein Blue AM emerges as a vital component for evaluating the well-being and viability of cellular populations. Through enzymatic hydrolysis within live cells, this dye undergoes a remarkable metamorphosis into a luminous fluorescent compound, emitting a vibrant blue fluorescence. This fluorescence empowers researchers to quantitatively assess live cell populations in a rapid and efficient manner—an indispensable tool for drug screening endeavors and investigations into cytotoxicity.
Intracellular Calcium Imaging: Embarking on the intriguing journey of intracellular calcium imaging, Calcein Blue AM assumes the role of a valuable ally in visualizing fluctuations in calcium levels within live cells. Following enzymatic cleavage, the dye showcases its prowess by chelating calcium ions and emitting fluorescence in response to changes in calcium concentration. This imaging technique provides a unique perspective into the intricate pathways of calcium signaling, offering insights into fundamental cellular processes such as muscle contraction, neurotransmitter release, and programmed cell death.
Cell Migration and Invasion Studies: Within the captivating realms of wound healing and cancer exploration, Calcein Blue AM stands as a fundamental tool for labeling live cells and facilitating in-depth investigations into cell migration and invasion dynamics. The dye’s stable and radiant blue fluorescence serves as a beacon, allowing for real-time tracking of cell movements under the watchful eye of a fluorescence microscope. This application is pivotal in unraveling the complex cellular responses to various treatments or environmental cues, shedding light on the dynamic behaviors exhibited by cells in different scenarios.
Flow Cytometry: In the realm of flow cytometry, Calcein Blue AM emerges as a versatile ally for labeling and scrutinizing live cells to unveil their distinct characteristics. Leveraging the dye’s unique fluorescence properties, researchers can discriminate between live and deceased cells based on esterase activity levels. This application is of paramount importance across diverse fields such as immunology, oncology, and cell biology, enabling meticulous studies of cell populations and their inherent attributes with precision and depth.
| XLogP3 | 1.1 |
| Hydrogen Bond Donor Count | 1 |
| Hydrogen Bond Acceptor Count | 12 |
| Rotatable Bond Count | 14 |
| Exact Mass | 465.12711055 g/mol |
| Monoisotopic Mass | 465.12711055 g/mol |
| Topological Polar Surface Area | 155Ų |
| Heavy Atom Count | 33 |
| Formal Charge | 0 |
| Complexity | 759 |
| Isotope Atom Count | 0 |
| Defined Atom Stereocenter Count | 0 |
| Undefined Atom Stereocenter Count | 0 |
| Defined Bond Stereocenter Count | 0 |
| Undefined Bond Stereocenter Count | 0 |
| Covalently-Bonded Unit Count | 1 |
| Compound Is Canonicalized | Yes |
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