3,3'-Dioctadecyloxacarbocyanine perchlorate | 34215-57-1

3,3’-Dioctadecyloxacarbocyanine perchlorate, commonly known as DiO, is a fluorescent dye used extensively in various fields of biological research. Here are some key applications of DiO:

Cell Membrane Labeling: DiO is often used to stain and label cell membranes due to its lipophilic nature. By incorporating into the lipid bilayer, it enables the visualization and tracking of cell membranes under fluorescence microscopy. This application is crucial in studying cell morphology, dynamics, and membrane-related processes.

Neuronal Tracing: DiO is utilized as a neuronal tracer in neurobiology to map neural pathways and connections. When applied to neurons, it diffuses through the lipid membranes along the neuronal processes, allowing researchers to study the architecture and connectivity of complex neural networks. This is vital for understanding neurodevelopmental processes and neural circuit functions.

Cell Fusion Studies: DiO can be used in cell fusion experiments to distinguish between different cell populations. By pre-labeling cells with DiO and then inducing fusion, researchers can track the mixing and integration of cellular components. This technique is beneficial in studying hybrid cells and understanding the mechanisms of cell fusion.

Live Cell Imaging: DiO is suitable for live-cell imaging due to its non-toxic and stable fluorescent properties. It allows for real-time monitoring of cellular processes such as vesicle trafficking, endocytosis, and exocytosis. This application provides valuable insights into dynamic cellular events and function in a live cellular context.