Tetramethylrhodamine-5-Iodoacetamide Dihydroiodide | CAS 114458-99-0

Catalog Number	A19-0008
Category	DNA Stains
Molecular Formula	C26H26I3N3O4
Molecular Weight	825.22

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Product Introduction

Tetramethylrhodamine-5-Iodoacetamide Dihydroiodide is an orange-fluorescent, thiol-reactive probe used for labeling DNA fragments.

Chemical Information
Product Specification
Application

Synonyms	Rho-IA; 5-TMRIA
Appearance	Solid Powder

Excitation	543 nm
Emission	567 nm

Tetramethylrhodamine-5-Iodoacetamide Dihydroiodide (TMR-IA), a fluorescent dye commonly utilized in biological research for labeling and detecting proteins and biomolecules, offers a myriad of applications:

Protein Labeling: Widely adopted for labeling cysteine residues in proteins, TMR-IA enables researchers to visualize and track protein dynamics with precision. This fluorescent probe covalently binds to thiol groups, serving as a potent tool for investigating protein folding, transport, and interactions. Through the use of TMR-IA, scientists can monitor real-time alterations in protein conformation and subcellular localization, unraveling intricate details of cellular processes.

Live-Cell Imaging: In live-cell imaging experiments, Tetramethylrhodamine-5-Iodoacetamide Dihydroiodide emerges as a popular choice for exploring intracellular dynamics. Its bright and enduring fluorescence characteristics make it ideal for prolonged imaging sessions, facilitating the observation of cellular phenomena such as endocytosis, exocytosis, and signal transduction. The versatility of TMR-IA across various microscopy techniques enhances its applicability in advancing cell biology research.

Fluorescence Resonance Energy Transfer (FRET) Studies: In the realm of FRET studies, TMR-IA plays a pivotal role as a FRET acceptor, shedding light on molecular interactions and distances between biomolecules. Coupled with a suitable FRET donor, this dye enables researchers to quantify changes in energy transfer efficiency, offering valuable insights into protein-protein interactions and conformational dynamics. This technique stands as a cornerstone for elucidating the structural and functional intricacies within cellular assemblies.

Flow Cytometry: Employed in flow cytometry for the analysis of protein expression and distribution in individual cells, TMR-IA’s vibrant fluorescence aids in distinguishing cells based on protein labeling, facilitating detailed population profiling. This application proves especially valuable in immunophenotyping and dissecting heterogeneous cell populations in both research and clinical settings, underscoring the versatility and utility of TMR-IA in cutting-edge biological studies.