TET azide, 6-isomer | CAS 1450752-90-5

TET azide, 6-isomer is a tetramethylrhodamine (TET)-based azide click chemistry reagent designed for efficient covalent labeling via azide-alkyne cycloaddition. As a fluorescing TET dye precursor, it enables incorporation of a bright, visible-wavelength fluorophore into proteins, peptides, oligonucleotides, and other biomolecule conjugates using established click labeling workflows. The reactive azide handle supports downstream probe construction for fluorescence microscopy, flow cytometry, and fluorescence-based assay development where robust dye conjugation is required.

1. Biomolecule Fluorescent Labeling

TET azide, 6-isomer is used by chemical biology and proteomics groups to generate fluorescently labeled biomolecules through click conjugation to alkyne-functional partners. Researchers commonly incorporate this reagent into antibody/ligand labeling workflows, peptide conjugates, and protein labeling strategies to create imaging reagents that can be tracked by fluorescence readout in microscopy and plate-based assays. The azide functionality provides a convenient handle for attaching the TET fluorophore to biomolecular scaffolds while maintaining compatibility with typical aqueous bioconjugation conditions used in labeling pipelines.

2. Fluorescence Microscopy Probes

TET azide, 6-isomer supports fluorescence microscopy reagent development where a TET dye reporter is needed for cellular or biomaterial imaging. Lab teams use it to prepare fluorescent conjugates for staining of biomolecule targets on surfaces, labeling of extracellular components, or visualization of tagged biomolecular constructs in fixed-sample imaging workflows. By installing the fluorophore through click chemistry, researchers can build microscopy probes with defined dye placement on alkyne-bearing targeting moieties, enabling consistent labeling for multi-step imaging experiments.

3. Flow Cytometry Dye Conjugates

TET azide, 6-isomer is frequently employed in flow cytometry workflows to produce fluorescent conjugates for labeling cells with alkyne-functional antibodies, ligands, or other binding reagents. Cytometry users rely on the azide click handle to attach the TET fluorophore to their chosen alkyne-bearing targeting component, generating stable fluorescent probes for population analysis and fluorescence-based readouts. This approach is particularly useful when researchers need a defined dye conjugation step to integrate into established staining panels and instrument-specific fluorescence detection schemes.

4. Oligonucleotide and Nucleic Acid Labeling

TET azide, 6-isomer is used in nucleic acid analysis and nucleic-acid probe construction to introduce a TET fluorescent reporter onto alkyne-modified oligonucleotides. Molecular biology groups incorporate the dye into hybridization probes and labeled primers for fluorescence imaging or fluorescence readout formats that require a covalent, well-defined fluorophore attachment. The azide functionality enables straightforward conjugation to alkyne-bearing oligos, supporting probe preparation for downstream fluorescence-based nucleic acid workflows.