Aminoallyl-UTP-ATTO-680

Aminoallyl-UTP-ATTO-680 is prominently used in fluorescent labeling of RNA for in vitro transcription assays. By incorporating this modified nucleotide during RNA synthesis, researchers can generate fluorescently labeled RNA molecules. This facilitates the tracking and quantification of RNA in various experimental systems, including gel electrophoresis and fluorescence microscopy. The ATTO-680 fluorophore is particularly advantageous due to its superior photostability and brightness, making it suitable for demanding applications that require prolonged observation and high-resolution imaging.

Another critical application of Aminoallyl-UTP-ATTO-680 is in the labeling of DNA during PCR amplification. By incorporating this nucleotide analogue into the DNA sequence, researchers can produce fluorescently labeled DNA fragments. This is highly beneficial for a variety of downstream applications, including real-time PCR, microarray analysis, and next-generation sequencing. The fluorescent label allows for the precise detection and quantification of DNA, enhancing the sensitivity and specificity of these techniques.

Aminoallyl-UTP-ATTO-680 is also valuable in the study of RNA-protein interactions. By labeling RNA molecules with this fluorescent probe, researchers can employ techniques such as fluorescence anisotropy, fluorescence resonance energy transfer (FRET), and co-immunoprecipitation to investigate the binding dynamics and affinity of RNA-binding proteins. The high photostability and brightness of ATTO-680 ensure that the fluorescent signal remains robust, even in complex biological environments, facilitating more accurate and reliable measurements.

Finally, Aminoallyl-UTP-ATTO-680 is utilized in single-molecule fluorescence studies. The incorporation of this fluorophore into RNA or DNA allows researchers to observe molecular interactions and dynamics at the single-molecule level using techniques such as single-molecule FRET (smFRET) and total internal reflection fluorescence (TIRF) microscopy. These studies provide unprecedented insights into the conformational changes, folding, and interactions of nucleic acids, contributing significantly to our understanding of molecular biology. The excellent optical properties of ATTO-680 make it an ideal choice for these highly sensitive and precise applications.