5-Propargylamino-dCTP-Texas Red

5-Propargylamino-dCTP-Texas Red is a Texas Red-labeled nucleotide analog designed for click-compatible DNA/RNA labeling workflows. Incorporating a propargylamino functionality enables copper-catalyzed azide-alkyne cycloaddition (CuAAC) after nucleic acid incorporation, supporting fluorescent tagging of nucleic acid strands and downstream hybridization or imaging reagents. The Texas Red fluorophore provides a red-emitting signal that is widely used in fluorescence microscopy and nucleic-acid-based assays, including workflows where additional clickable handles are needed for multicolor or modular probe construction.

1. Clickable Nucleic Acid Labeling

5-Propargylamino-dCTP-Texas Red is used by molecular biology and chemical biology groups to introduce a click-reactive, Texas Red-bearing cytidine analog into DNA or RNA during nucleic acid synthesis. Researchers then use the incorporated alkyne functionality to attach azide-functional reporters, affinity tags, or secondary fluorophores via CuAAC, enabling modular fluorescent labeling beyond what is achievable with a single dye incorporation step. This approach is commonly applied when labeled nucleic acids must be further functionalized for imaging, pull-down workflows, or construction of multicomponent nucleic acid probes.

2. Fluorescence In Situ Hybridization Probes

5-Propargylamino-dCTP-Texas Red supports the development of fluorescent in situ hybridization (FISH) probes by providing a red-emitting Texas Red label within nucleic acid probe strands. After probe synthesis, the click handle can be leveraged to append additional azide-bearing components, such as solubilizing groups or secondary labeling elements, to tailor probe performance for specific hybridization formats and imaging pipelines. Laboratories using microscopy-based nucleic acid localization workflows benefit from the modularity of adding clickable functionality to the probe design while maintaining a robust red fluorescence readout.

3. Flow Cytometry DNA/RNA Assays

5-Propargylamino-dCTP-Texas Red can be incorporated into nucleic acid targets or assay constructs for fluorescence-based analysis by flow cytometry, where red emission from Texas Red supports multiplexing with other fluorophores. In nucleic-acid-centric workflows, the click-reactive handle enables post-synthesis conjugation to azide-functional components that improve assay organization, such as affinity capture moieties for enrichment or standardized labeling for consistent staining. This makes the reagent useful for assay development teams building nucleic acid staining strategies that require both a fluorescent label and a programmable conjugation step.

4. Modular Fluorescent Probe Construction

5-Propargylamino-dCTP-Texas Red is frequently used in fluorescent probe development when researchers need a nucleic-acid-derived fluorescent scaffold that can be diversified after synthesis. By first generating Texas Red-labeled nucleic acids containing the propargylamino functionality, teams can subsequently click on azide-functional imaging reagents, targeting ligands, or assay components to create customized probe architectures for specific experimental designs. This modular strategy is especially valuable for laboratories iterating probe formats for microscopy, hybridization assays, and nucleic acid-based molecular imaging workflows.