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Rhodamine-12-dCTP
| Catalog Number | A07-0041 |
| Category | RNA/DNA Labeling |
| Molecular Formula | C39H42N7O18P3(free acid) |
| Molecular Weight | 989.71 (free acid) |
| Catalog Number | Size | Price | Quantity |
|---|---|---|---|
| A07-0041 | -- | $-- |
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Product Introduction
Rhodamine-12-dCTP, the fluorescent nucleotide analog frequently employed in molecular biology research for the purpose of labeling nucleic acids, renders itself a prominent tool. Often operating in combination with other fluorescent dyes, this molecule facilitates the study of DNA-protein interactions as well as gene expression. A bright red emission compliments the molecule, serving visualization purposes during PCR and real-time PCR.
Chemical Information |
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| Synonyms | Rhodamine-X-5-propargylamino-dCTP; Rho-green-X-dCTP, RhG-dCTP, R110-dCTP; 5/6-Rhodamine-X-5-propargylamino-2'-deoxycytidine-5'-triphosphate, Triethylammonmium salt |
| Purity | ≥ 95 % by HPLC |
| pH | 7.5 ±0.5 |
| Appearance | orange solution in water |
- Product Specification
- Application
| Spectroscopic Properties | λexc 505 nm, λem 530 nm, ε 85.0 L mmol-1 cm-1 (Tris-HCl pH 7.5) |
| Storage | store at-20 °CShort term exposure (up to 1 week cumulative) to ambient temperature possible. |
Rhodamine-12-dCTP, a fluorescently labeled nucleotide widely utilized in molecular biology, exhibits a plethora of applications in the field. Here are four key applications:
DNA Sequencing: Serving as a cornerstone of genetic analysis, Rhodamine-12-dCTP plays a pivotal role in DNA sequencing methodologies such as Sanger sequencing. By attaching this fluorescent label to DNA fragments, researchers can precisely identify nucleotide sequences as they migrate through a capillary electrophoresis system. The unmatched sensitivity and specificity of this label assist in accurately determining DNA sequences, proving invaluable for genetic research and diagnostic purposes.
Quantitative PCR (qPCR): Venturing into the realm of quantitative analysis, Rhodamine-12-dCTP finds its niche in qPCR assays devised to monitor the amplification of specific DNA targets. The generation of a fluorescent signal during PCR cycles offers real-time insights into the quantity of the target DNA, essential for studies on gene expression, pathogen detection, and genetic quantification pursuits.
Fluorescence In Situ Hybridization (FISH): A technique of profound significance in genetic mapping, Rhodamine-12-dCTP is utilized to label DNA probes employed in FISH to hybridize with precise chromosomal regions. The brightly colored probes enable researchers to visually map genes or other DNA sequences within chromosomes, playing a pivotal role in genetic mapping, detection of chromosomal anomalies, and advancements in cancer research.
Flow Cytometry: Injecting innovation into cell analysis, Rhodamine-12-dCTP integrates into DNA for usage in flow cytometry assays geared towards analyzing cell cycle stages and DNA content. The unique fluorescent properties of Rhodamine-12-dCTP aid in distinguishing cells at various cell cycle stages based on their DNA content. This application holds critical significance in studies of cell biology, cancer research, and proliferation assays, shedding light on the intricate dynamics of cellular processes.
Applications of Fluorescent Probes & Dyes
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