Aminoallyl-dUTP-XX-Cy3 (ethyl)

Aminoallyl-dUTP-XX-Cy3 (ethyl), a versatile fluorescent nucleotide analog, finds applications in various molecular biology contexts. Here are four key applications:

Fluorescent Labeling: Widely utilized in DNA and RNA fluorescent labeling, Aminoallyl-dUTP-XX-Cy3 (ethyl) plays a crucial role in molecular biology. By integrating this modified nucleotide into nucleic acids during PCR or in vitro transcription, researchers can generate fluorescently labeled probes. These probes are indispensable for techniques like fluorescence in situ hybridization (FISH) and microarray analysis, facilitating the visualization and quantification of specific nucleic acid sequences.

Chromatin Immunoprecipitation (ChIP) Assays: In ChIP assays, Aminoallyl-dUTP-XX-Cy3 (ethyl) serves as a valuable tool for labeling DNA fragments bound to specific proteins. By integrating the fluorescent nucleotide into DNA during crosslinking and fragmentation stages, researchers can track and analyze protein-DNA interactions. This methodology aids in unraveling gene regulation mechanisms and identifying transcription factor binding sites, offering profound insights into cellular processes.

Genetic Mapping: Incorporating Aminoallyl-dUTP-XX-Cy3 (ethyl) into DNA enables detailed genetic mapping through fluorescent microscopy, a fundamental aspect of molecular biology research. Scientists can leverage labeled DNA to construct high-resolution physical maps and visualize chromosomal arrangements and abnormalities. This technique is particularly beneficial in cytogenetic studies and the detection of genetic disorders, enhancing our understanding of genetic variations.

Quantitative PCR (qPCR): In quantitative PCR assays, Aminoallyl-dUTP-XX-Cy3 (ethyl) plays a pivotal role in facilitating fluorescence-based detection and quantification of PCR products. By integrating the fluorescent nucleotide into amplified DNA, real-time monitoring of the PCR process becomes achievable. This enhances the accuracy and sensitivity of qPCR, enabling precise measurement of gene expression levels and genetic variations, contributing significantly to molecular biology research.