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Aminoallyl-dUTP-XX-AF555
| Catalog Number | A07-0027 |
| Category | RNA/DNA Labeling |
| Molecular Formula | C24H41N5O16P3-AF555(freeacid) |
| Molecular Weight | 1577.5 |
* Please be kindly noted products are not for therapeutic use. We do not sell to patients.
Product Introduction
Recommended Aminoallyl-dUTP-XX-AF555/dTTP ratio for PCR and Nick Translation: 30-50% Aminoallyl-dUTP-XX-AF555/ 70-50% dTTP
Chemical Information
Product Specification
Application
| Purity | ≥95% (HPLC) |
| pH | 7.5 ±0.5 |
| Appearance | sterile pink solution in 10 mM Tris-HCl |
| Spectroscopic Properties | Applications:Incorporation into DNA/cDNA by -PCR with Taq polymerase in-house data -Nick Translation with DNAse I/ DNA Polymerase I in-house data |
| Storage | store at-20 °CShort term exposure (up to 1 week cumulative) to ambient temperature possible. |
Aminoallyl-dUTP-XX-AF555, a fluorescent nucleotide analog utilized in molecular biology research, offers a myriad of applications. Here are four key uses creatively:
Fluorescent Labeling in PCR: Incorporating Aminoallyl-dUTP-XX-AF555 into PCR products facilitates the generation of fluorescently labeled DNA strands. This groundbreaking technique allows for the real-time visualization and quantification of amplified DNA fragments during PCR assays. Its exceptional sensitivity and specificity empower a wide range of applications, including the nuanced analysis of gene expression dynamics and precise diagnostic testing.
Microarray Analysis: Stepping into the realm of microarray experiments, Aminoallyl-dUTP-XX-AF555 emerges as a pivotal player in labeling cDNA or RNA samples. This elemental step enables researchers to uncover and compare gene expression profiles across diverse samples or experimental conditions – a critical aspect of molecular biology research. With the bright fluorescence emitted by Aminoallyl-dUTP-XX-AF555, researchers are empowered to conduct high-throughput screening efficiently, identifying differentially expressed genes with unprecedented accuracy.
In Situ Hybridization: Delving deeper into the intricacies of in situ hybridization techniques, Aminoallyl-dUTP-XX-AF555 showcases its prowess in visualizing the precise localization of specific nucleic acid sequences within tissue samples or cell sections. By hybridizing labeled probes to target genes, researchers unveil intricate spatial details of gene expression patterns, shedding light on fundamental aspects of developmental biology and pathology. This application stands as a cornerstone in unraveling the complexities of gene regulation and expression dynamics.
Flow Cytometry: Embracing the avant-garde applications of flow cytometry, Aminoallyl-dUTP-XX-AF555 emerges as a vital tool for labeling DNA within cells for in-depth analysis. This innovative approach enables researchers to explore cell cycle progression, apoptosis rates, and DNA content variations within heterogeneous cell populations. Thanks to the vivid fluorescence emitted by AF555, the precision and efficiency of sorting and analyzing cells are significantly enhanced, opening new avenues for groundbreaking discoveries in diverse research domains.
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